CD134 Antibody | OX-86

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CD134 Antibody | OX-86 gallery image 1

Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134 (MCA1420)

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CD134 Antibody | OX-86 gallery image 2

Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134:Alexa Fluor® 647 (MCA1420A647)

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CD134 Antibody | OX-86 gallery image 3

Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134:RPE (MCA1420PE)

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CD134 Antibody | OX-86 gallery image 4

Staining of stimulated rat spleen cells with Rat anti Mouse CD134:Azide Free (MCA1420XZ)

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  • CD134 Antibody | OX-86 thumbnail image 1
  • CD134 Antibody | OX-86 thumbnail image 2
  • CD134 Antibody | OX-86 thumbnail image 3
  • CD134 Antibody | OX-86 thumbnail image 4
  • Rat anti Mouse CD134:FITC
  • Rat anti Mouse CD134:FITC
  • Rat anti Mouse CD134:Alexa Fluor® 647
  • Rat anti Mouse CD134:FITC
  • Rat anti Mouse CD134
  • Rat anti Mouse CD134:FITC
  • Rat anti Mouse CD134:Alexa Fluor® 647
  • Rat anti Mouse CD134:RPE
  • Rat anti Mouse CD134:RPE
  • Rat anti Mouse CD134
  • Rat anti Mouse CD134
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-86
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1420FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1420F
    MCA1420GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA1420GA
    MCA1420C, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA1420
    MCA1420FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA1420FB
    MCA1420PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1420PE
    MCA1420A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1420A647
    MCA1420FTFdatasheet pdfdatasheet pdf25 µg
    MCA1420FT
    MCA1420TC, Fdatasheet pdfdatasheet pdf25 µg
    MCA1420T
    MCA1420PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1420PET
    MCA1420A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1420A647T
    MCA1420FAFdatasheet pdfdatasheet pdf50 µg
    MCA1420FA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD134 antibody, clone OX-86 recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.

      Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      CHO cells transfected with murine OX40
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 50 µg, 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 50 µg, 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 50 µg, 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 1 mg/ml
      Pack Size: 50 µg, 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 1 mg/ml
      Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised AO rats were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Pack Size: 50 µg, 25 µg, 0.1 mgStore at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µg, 25 µg, 0.1 mgStore at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µg, 25 µg, 0.1 mgStore at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mg, 25 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µg, 25 µg, 0.1 mgStore at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mg, 25 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mg, 25 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      regulation of protein kinase activity
      integral to membrane
      regulation of apoptosis
      negative regulation of transcription factor activity
      external side of plasma membrane
      cellular defense response
      negative regulation of cytokine secretion
      positive regulation of immunoglobulin secretion
      negative regulation of gene-specific transcription
      tumor necrosis factor receptor activity
      inflammatory response
      positive regulation of B cell proliferation
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD134 Antibody Formats

    Formats Clone Applications Sizes available
    CD134 Antibody : RPE OX-86 F 100 Tests | 25 Tests
    CD134 Antibody : Alexa Fluor® 647 OX-86 F 100 Tests/1ml | 25 Tests/0.25ml
    CD134 Antibody : Purified OX-86 C, F 25 µg | 0.25 mg | 0.1 mg
    CD134 Antibody : FITC OX-86 F 25 µg | 0.5 mg | 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

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      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
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          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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