Trastuzumab antibody | AbD18018

Human anti Trastuzumab

Product Type
Monoclonal Antibody
Clone
AbD18018
Isotype
HuCAL Fab monovalent
Specificity
Trastuzumab

Product Code Applications Pack Size List Price Your Price Qty
HCA168
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SDS Safety Datasheet SDS
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HCA168G
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Human Anti-Trastuzumab Antibody, clone AbD18018, is a paratope specific, high affinity, anti-idiotypic antibody that recognizes the monoclonal antibody drug trastuzumab and inhibits it binding to its target. The antibody can be used to measure the levels of trastuzumab and biosimilar products in bioanalytical assays. Human Anti-Trastuzumab Antibody, clone AbD18018 is a fully human recombinant monoclonal antibody in monovalent Fab format and is suitable as a capture or detection antibody paired with antibody clone AbD16712 (HCA166) in a pharmacokinetic (PK) bridging ELISA, to measure free drug in preclinical research and clinical trials using patient sera.

Human Anti-Trastuzumab Antibody, clone AbD18018 is also available in full immunoglobulin format as IgG1 isotype (HCA177) or IgG4 isotype (HCA270) in order to offer assay developers increased flexibility in assay design.

Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

View a summary of all Anti-Trastuzumab Antibodies.

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E.coli.. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Trastuzumab
Affinity
Human anti Trastuzumab antibody, clone AbD18018 is an ultra-high affinity idiotype antibody that recognizes the Herceptin humanised monoclonal antibody. The monovalent intrinsic affinity of Human anti Trastuzumab antibody, clone AbD18018 is ~0.02nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Herceptin.
Approx. Protein Concentrations
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml.
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Herceptin is a registered trademark of Genentech, Inc.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product may be used in a direct ELISA or as a capture reagent in a sandwich ELISA together with HCA166 as the detection reagent, when conjugated to HRP.
Protocol: PK bridging ELISA to measure free drug.

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Synonyms
Herceptin
RRID
AB_11152944
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro. research testing services. Any re-sale or any other form of commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"