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IL-8 antibody | 8M6

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Anti Sheep Interleukin-8 Antibody, clone 8M6 thumbnail image 1 Anti Sheep Interleukin-8 Antibody, clone 8M6 thumbnail image 2 Anti Sheep Interleukin-8 Antibody, clone 8M6 thumbnail image 3
Anti Sheep Interleukin-8 Antibody, clone 8M6 gallery image 1
Published customer image:
Mouse anti Sheep interleukin-8 antibody, clone 8M6 (MCA1660) used, in conjunction with Rabbit anti Sheep interleuin-8 antibody (AHP425 ) for the measurement of ovine CXCL8 in embryonal bovine lungs by ELISA.
Image caption:
Analysis of bovine TLR5 activity in bovine epithelial cells.
The bovine EBL cell line was stably transfected with a bTLR5 clone and CXCL8 secretion assayed following challenge with flagellin. (A) CXCL8 levels from EBLs with and without transfection of bTLR5. H7 flagellin at a range of concentrations was added to the cells and CXCL8 levels were measured by ELISA following overnight incubation. Transfection with bTLR5 resulted in significantly higher levels of CXCL8 being produced by the bTLR5+ cells with addition of 50-50,000 ng/mL of H7 flagellin (p < 0.001). The data shown are the means and 95% confidence intervals. (B) Analysis of CXCL8 production from EBL-TLR5 in response to addition of native H7 or an H7 flagellin preparation from which the majority of LPS has been removed. Medians and interquartile ranges are shown. (C) Secreted CXCL8 following addition of WT and mutated H7 flagellins to EBLs with transfected bTLR5. 50 ng/mL of WT-H7 and mutated flagellins were added to the EBLs transfected with bTLR5 and incubated overnight. CXCL8 was measured by ELISA. Addition of the R90T, R90A and L500A/I504A variants led to significantly lower levels of cytokine release (p <0.001) relative to WT-H7 stimulation (asterisks). R90T showed a significantly lower induction than R90A (p <0.001). Medians and interquartile ranges are shown. (D) Secreted CXCL8 following addition of WT H7 and the R90T flagellin mutant to EBL cells. A range of flagellin concentrations was incubated overnight with the cells and CXCL8 measured by ELISA. R90T flagellin demonstrated significantly reduced levels of CXCL8 activation at 50 ng/mL and 500 ng/mL (p <0.001), marked by asterisks. The data shown are the means and 95% confidence intervals. All CXCL8 data shown is from a minimum of three biological replicates.

From: Tahoun A, et al.
Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 2015 Feb 19;46:9.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Sheep Interleukin-8 Antibody, clone 8M6 gallery image 2
Published customer image:
Mouse anti Sheep interleukin-8 antibody, clone 8M6 (MCA1660) used for the identification of IL-8 expressing porcine vaginal epithelial cells in a chlamydial infection model by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
IHC staining of cox-2 and IL-8 in vaginal tissue from day 3 PI, and control tissues. a Vagina, day 3 PI. Positive staining in numerous superficial epithelial cells for cox-2 (arrows); b Vagina from control tissue, showing positive staining for cox-2 in single superficial epithelial cells (arrow); c Vagina, day 3 PI. Positive (red) staining in numerous superficial epithelial cells for IL-8 (arrows); d Vagina from control tissue showing positive staining for IL-8 in a single epithelial cell (arrow).

From: Erneholm K, Lorenzen E, B7oslash;je S, Olsen AW, Andersen P, Cassidy JP, Follmann F, Jensen HE, Agerholm JS.
Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection with Chlamydia trachomatis serovar D.
BMC Vet Res. 2016 Sep 10;12(1):200.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Sheep Interleukin-8 Antibody, clone 8M6 gallery image 3
Published customer image:
Mouse anti Ovine Interleukin-8 antibody, clone 8M6 (MCA1660) used as a coating reagent in a sandwich ELISA to evaluate interleukin-8 levels in sheep neutrophil supernatants.
Image caption:
IL-8 production in PMN upon E. bovis exposure. Supernatants of PMN (1 × 106 per sample; n = 3) treated with TLR2 and TLR4 antibodies and exposed to live E. bovis sporozoites (1:1 ratio; 2 h) were assessed for the presence of IL-8 by ELISA analysis. TLR2-treated PMN exposed to sporozoites showed a significant increase in IL-8 production (* p <0.05) when compared to PMN in media. Likewise, a significant increase in IL-8 production (** p <0.01) was observed in the same experimental condition when compared to the respective control without exposure to E. bovis. Data are represented as the mean of 3 replicates ± SD and were analyzed using an unpaired Student’s t-test using GraphPad Prism V.8.4.3 (GraphPad Software Inc., San Diego, CA, USA). p-value notation; * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.

From: Muñoz-Caro T, Gibson AJ, Conejeros I, Werling D, Taubert A, Hermosilla C.
The Role of TLR2 and TLR4 in Recognition and Uptake of the Apicomplexan Parasite Eimeria bovis and Their Effects on NET Formation.
Pathogens. 2021 Jan 24;10(2):118.
doi: 10.3390/pathogens10020118.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Sheep Interleukin-8

Product Type
Monoclonal Antibody
Clone
8M6
Isotype
IgG2a
Specificity
IL-8

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Product Code Applications Pack Size List Price Your Price Qty
MCA1660
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * FN * WB 0.25 mg loader
List Price Your Price
loader

Mouse anti Ovine Interleukin-8 antibody, clone 8M6 recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 79 amino acid ~9-11 kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti ovine Interleukin-8 antibody, clone 8M6 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.

Responses to infectious stimuli may vary among ovine species, the response to Mannheimia haemolytica, a causative agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exaggerated in Bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection (Herndon et al. 2010).

Mouse anti ovine interleukin-8 antibody, clone 8M6 has been utilized to identify cells and cell types expressing IL-8 in inflamed porcine tissue (Laursen et al. 2014) showing here also that neutrophils are the predominant cell type expressing IL-8 whilst epithelial and endothelial cells in the vicinity of inflammatory lesions also express the cytokine. Clone 8M6 neutralizes the bioactivity of ovine IL-8.
Mouse anti Ovine Interleukin-8 antibody, clone 8M6 has been used in conjunction with Rabbit anti Sheep Interleukin-8 antibody (AHP425) for the development of a sensitive ELISA to measure IL-8 concentrations in bovine samples (Cronin et al. 2015).

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Rabbit
Bovine
Pig
Mustelid
Ferret
Mink
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Recombinant ovine IL-8.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml
Flow Cytometry 1 1/10
Functional Assays 2
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2 Removal of the preservative is recommended prior to use in functional assays.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
ELISA
Mouse anti Sheep interleukin-8 antibody, clone 8M6 may be used in combination with AHP425 in sandwich ELISA assays for ovine IL-8.

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References for IL-8 antibody

  1. Caswell, J.L. et al. (1998) Expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis.
    Vet Pathol. 35: 124-31.
  2. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
    Vet Immunol Immunopathol. 88 (3-4): 111-22.
  3. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.
    Clin Diagn Lab Immunol. 9 (6): 1229-34.
  4. Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infection.
    Infect Immun. 78: 3578-84.
  5. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  6. Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
    Vet Immunol Immunopathol. 124: 367-78.
  7. Bonnefont, C.M. et al. (2012) Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant.
    Physiol Genomics. 44: 403-16.
  8. Jensen, P.V. et al. (2003) Cytokine profiles in adult mink infected with Aleutian mink disease parvovirus.
    J Virol. 77: 7444-51.
    9. View The Latest Product References
  9. Singh, B. et al. (2004) Depletion of pulmonary intravascular macrophages inhibits acute lung inflammation.
    Am J Physiol Lung Cell Mol Physiol. 286: L363-72.
  10. Redondo, E. et al. (2013) Induction of Interleukin-8 and Interleukin-12 in Neonatal Ovine Lung Following Experimental Inoculation of Bovine Respiratory Syncytial Virus.
    J Comp Pathol. 150: 434-48.
  11. Laursen, H. et al. (2014) Immunohistochemical detection of interleukin-8 in inflamed porcine tissues.
    Vet Immunol Immunopathol. 159: 97-102.
  12. Boulanger, D. et al. (2003) Increased nuclear factor kappaB activity in milk cells of mastitis-affected cows.
    J Dairy Sci. 86: 1259-67.
  13. Cronin, J.G. et al. (2015) Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.
    Am J Reprod Immunol. 73 (4): 372-82.
  14. Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
    Vet Immunol Immunopathol. 168 (1-2): 97-102.
  15. Rainard, P. et al. (2008) The chemokine CXCL3 is responsible for the constitutive chemotactic activity of bovine milk for neutrophils.
    Mol Immunol. 45 (15): 4020-7.
  16. Stinson LF et al. (2014) Effects of cytokine-suppressive anti-inflammatory drugs on inflammatory activation in ex vivo human and ovine fetal membranes.
    Reproduction. 147 (3): 313-20.
  17. Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
    Vet Res. 46: 9.
  18. Erneholm, K. et al. (2016) Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection with Chlamydia trachomatis serovar D.
    BMC Vet Res. 12 (1): 200.
  19. Melville, J.M. et al. (2017) Human amnion epithelial cells modulate the inflammatory response to ventilation in preterm lambs.
    PLoS One. 12 (3): e0173572.
  20. Magiri, R. et al. (2019) Innate immune response profiles in pigs injected with vaccine adjuvants polydi(sodium carboxylatoethylphenoxy)phosphazene (PCEP) and Emulsigen.
    Vet Immunol Immunopathol. 209: 7-16.
  21. Muñoz-Caro, T. et al. (2021) The Role of TLR2 and TLR4 in Recognition and Uptake of the Apicomplexan Parasite Eimeria bovis and Their Effects on NET Formation.
    Pathogens. 10 (2): 118.
  22. Mittal, P. et al. (2019) Outer kinetochore protein Dam1 promotes centromere clustering in parallel with Slk19 in budding yeast.
    Chromosoma. 128 (2): 133-48.

ELISA

Flow Cytometry

Immunoprecipitation

Synonyms
CXCL8
RRID
AB_322152
UniProt
P36925
Entrez Gene
IL8
GO Terms
GO:0005153 interleukin-8 receptor binding
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0008009 chemokine activity
GO:0030155 regulation of cell adhesion
GO:0030593 neutrophil chemotaxis
GO:0045091 regulation of retroviral genome replication
GO:0050930 induction of positive chemotaxis

MCA1660

149224 151836

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