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IL-8 Antibody

IL-8 Antibody gallery image 1

Published customer image:
Rabbit anti Sheep interleukin-8 antibody (AHP425) used in conjunction with Mouse antiSheep interleukin-8 antibody, clone 8M6 (MCA1660 ) for the measurement of ovine CXCL8 in embryonal bovine lungs by ELISA.
Image caption:
Analysis of bovine TLR5 activity in bovine epithelial cells.
The bovine EBL cell line was stably transfected with a bTLR5 clone and CXCL8 secretion assayed following challenge with flagellin. (A) CXCL8 levels from EBLs with and without transfection of bTLR5. H7 flagellin at a range of concentrations was added to the cells and CXCL8 levels were measured by ELISA following overnight incubation. Transfection with bTLR5 resulted in significantly higher levels of CXCL8 being produced by the bTLR5+ cells with addition of 50-50,000 ng/mL of H7 flagellin (p?<?0.001). The data shown are the means and 95% confidence intervals. (B) Analysis of CXCL8 production from EBL-TLR5 in response to addition of native H7 or an H7 flagellin preparation from which the majority of LPS has been removed. Medians and interquartile ranges are shown. (C) Secreted CXCL8 following addition of WT and mutated H7 flagellins to EBLs with transfected bTLR5. 50 ng/mL of WT-H7 and mutated flagellins were added to the EBLs transfected with bTLR5 and incubated overnight. CXCL8 was measured by ELISA. Addition of the R90T, R90A and L500A/I504A variants led to significantly lower levels of cytokine release (p?<0.001) relative to WT-H7 stimulation (asterisks). R90T showed a significantly lower induction than R90A (p?<0.001). Medians and interquartile ranges are shown. (D) Secreted CXCL8 following addition of WT H7 and the R90T flagellin mutant to EBL cells. A range of flagellin concentrations was incubated overnight with the cells and CXCL8 measured by ELISA. R90T flagellin demonstrated significantly reduced levels of CXCL8 activation at 50 ng/mL and 500 ng/mL (p?<0.001), marked by asterisks. The data shown are the means and 95% confidence intervals. All CXCL8 data shown is from a minimum of three biological replicates.

From: Tahoun A, et al.
Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 2015 Feb 19;46:9.

  • IL-8 Antibody thumbnail image 1
  • Rabbit anti Sheep Interleukin-8
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP425Edatasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Sheep Interleukin-8 antibody recognises ovine interleukin-8, also known asIL-8, C-X-C motif chemokine 8 or Chemokine (C-X-C motif) ligand 8. Interleukin-8 is a 79 amino acid ~11kDa secreted cytokine chemotactic for neutrophils, basophils and T-cells but not monocytes.

      No cross-reactivity is seen with ovine IL-1 beta, IL-6, MCP or TNF alpha.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BovineExpected from Sequence
      GoatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to ovine IL-8 were raised by repeated immunisation of rabbits with highly purified antigen.
    • Preservative Stabilisers
      0.1%Sodium Azide
    • Immunogen
      Recombinant ovine IL-8.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      extracellular space
      neutrophil chemotaxis
      immune response
      inflammatory response
      regulation of retroviral genome replication
      induction of positive chemotaxis
      chemokine activity
      cell cycle arrest
      regulation of cell adhesion
      interleukin-8 receptor binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      The antibody may be used in a sandwich ELISA as a detection reagent for ovine IL-8, in combination with MCA1660 as capture antibody.

      N.B. It is recommended that AHP425 and any detection antibody are diluted in 10% normal mouse serum prior to use in ELISA.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-8 Antibody Formats

    Formats Applications Sizes available
    IL-8 Antibody : Serum E 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
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      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • IL-8 Antibody thumbnail image 1
              • Application NameReference Images


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