IL-6 antibody | 4B6
Mouse anti Ovine Interleukin-6 antibody, clone 4B6 (MCA1659) used as a capture antibody in a sandwich ELISA for the evaluation of IL-6 expression in response to LPS treatment using Rabbit anti Ovine IL-6 antibody (AHP424) as a detection reagent.
Image caption:
Fetal inflammatory response to lipopolysaccharide.
Blue, control group (n = 5); red, LPS group (n = 10); Mean ± SD. *, P = 0.001 versus control.
From: Herry CL, Cortes M, Wu H-T, Durosier LD, Cao M, Burns P, et al.. (2016)
Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.
PLoS ONE 11(4): e0153515.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep interleukin 6 antibody, clone 4B6 (MCA1659) used as a capture reagent for the measurement of interleukin 6 in an ELISA with Rabbit anti Sheep polyclonal antibody (AHP424) as a detection reagent using lamb sera samples. Interleukin 1β was detected with Mouse anti Sheep IL-1β antibody (MCA1658) as a capture reagent and Rabbit anti IL-1β polyclonal antibody (AHP423) as a detection reagent. Visualisation was achieved with a Goat anti Rabbit IgG antibody conjugated to HRP in both cases.
Image caption:
Systemic and diaphragm cytokine responses. Plasma IL-1β (A) and IL-6 (B) protein content. Values are mean ± SEM. Diaphragm IL-1β (C) and IL-6 (D) mRNA expression. Values are median (with 10th and 90th centiles). * p<0.05 compared to Sal/Sal; # p<0.05 compared to Sal/LPS.
From: Karisnan K, Bakker AJ, Song Y, Noble PB, Pillow JJ, et al. (2015)
Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
PLoS ONE 10(4): e0124390.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep Interleukin-6 antibody, clone 4B6 (MCA1659) used as a capture antibody for the measurement of IL-6 levels in sheep plasma by ELISA with Rabbit anti Sheep IL-6 polyclonal antibody (AHP424) used as a detection reagent.
Image caption:
Comparison of plasma concentrations of (A) IL-10 and (B) IL-6 measured over time in arthritic sheep treated and untreated with mesenchymal precursor cells (MPC). Each point represents the mean ± SEM of 7–8 sheep.**** Indicates a statistically significant difference between groups at that time point, p ≤ 0.0001.
From: Dooley LM, Abdalmula A, Washington EA, Kaufman C, Tudor EM, Ghosh P, et al. (2015)
Effect of Mesenchymal Precursor Cells on the Systemic Inflammatory Response and Endothelial Dysfunction in an Ovine Model of Collagen-Induced Arthritis.
PLoS ONE 10(5): e0124144.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep IL-6 antibody, clone 4B6 (MCA1659) used to assess IL-6 levels in plasma samples by ELISA.
Image caption:
Effect of IA LPS and IV hAECs on systemic cytokine expression. Fetal plasma IL-6 (A) and IL-10 (B) concentrations were not significantly different between groups. Data are expressed as mean ± SEM.
From: Westover A, Melville JM, McDonald C, Lim R, Jenkin G, Wallace EM, Moss TJ.
Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.
Front Physiol. 2017 Nov 6;8:871.
doi: 10.3389/fphys.2017.00871.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep Interleukin-6 antibody, clone 4B6 (MCA1659) used as a capture antibody for the evaluation if IL-6 levels in sheep serum by sandwich ELISA
Image caption:
Effects of the red orange and lemon extract (RLE on interleuchin-6 (IL-6) (a) and interleuchin-1β (IL-1β) (b) levels, expressed as pg/mL, in control group and RLE group after 40days of treatment. Data are shown as mean ± standard deviation (DS) and were compared by ANOVA (p<.05 versus control).
From: Sara Damiano, Consiglia Longobardi, Angela Salzano, Livia D’Angelo, Margherita Amenta, Aristide Maggiolino, Pasquale De Palo, Salvatore Claps, Domenico Rufrano, Francesco Iannaccone, Roberta Matera & Roberto Ciarcia (2022).
Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambs.
Italian Journal of Animal Science, 21:1, 934-942.
doi: 10.1080/1828051X.2022.2056527
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep Interleukin-6 antibody, clone 4B6 (MCA1659) used as a coating reagent to detect interleukin-6 in fetal sheep by ELISA
Image caption:
Fetal systemic inflammatory response to intravenous lipopolysaccharide (LPS) injection after the baseline and at 24 h: the impact of vagus nerve manipulation. Vx, bilateral cervical vagotomy during surgical instrumentation; LPS400 and LPS800 indicate the respective intravenous dose of LPS in ng/fetus/day given after the baseline and 24 h later; the efferent VNS group received Vx and LPS400 as the Vx + LPS400 group was followed by VNS treatment around the LPS administration at days 1 and 2.
From: Cao M, Kuthiala S, Jean KJ, Liu HL, Courchesne M, Nygard K, Burns P, Desrochers A, Fecteau G, Faure C, Frasch MG.
The Vagus Nerve Regulates Immunometabolic Homeostasis in the Ovine Fetus near Term: The Impact on Terminal Ileum.
Biology (Basel). 2024 Jan 9;13(1):38.
doi: 10.3390/biology13010038.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep interleukin 6 antibody, clone 4B6 (MCA1659) used as a capture reagent in an ELISA to measure levels of IL-6 in lamb serum samples.
Image caption:
Plasma IL-1β (A) and IL-10 (B) secretion in lambs fed with a control maize–barley-based concentrated diet (CON) and with 15% of hazelnut skin (HS) as a maize substitute for the concentrate diet at 15, 35, and 56 days. a,b indicate significant differences among feeding treatments and time of sampling (p < 0.10 IL-1β, and for p < 0.05 for IL-10, respectively).
From: Ciliberti MG, Santillo A, Caroprese M, Della Malva A, Natalello A, Bertino A, Albenzio M, Sevi A.
Role of hazelnut skin supplementation on plasma antioxidant status and cytokine profile in growing lambs.
Front Vet Sci. 2024 Feb 1;11:1340141.
doi: 10.3389/fvets.2024.1340141.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep Interleukin-6 antibody, clone 4B6 (MCA1659) used to evaluate IL-6 expression in ovine brain by flow cytometry.
Image caption:
Evaluation of IL-1β expression in different brain regions evaluated after sham or HI (and vehicle or WIN treatment) in fetal lambs. Samples from both hemispheres were analyzed in triplicate by flow cytometry (n = 6) for each animal and condition. Values given as the mean ± SD of the percentage of IL-1β positive cells. (*) ANOVA p <0.05 vs. sham and HI+WIN; (**) ANOVA p <0.01 vs. sham and HI+ WIN; (#) ANOVA p <0.05 vs. HI+VEH. Sham, n = 6; HI + VEH, n=6; HI+WIN, n = 6. (C: cortex; BN: basal nuclei; HT: hypothalamus; T: thalamus; HC: hippocampus; CB: cerebellum).
From:Alonso-Alconada D, Álvarez FJ, Goñi-de-Cerio F, Hilario E, Álvarez A.
Cannabinoid-mediated Modulation of Oxidative Stress and Early Inflammatory Response after Hypoxia-Ischemia.
Int J Mol Sci. 2020 Feb 14;21(4):1283.
doi: 10.3390/ijms21041283.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep Interleukin-6 antibody, clone 4B6 (MCA1659) used to evaluate IL-6 expression in ovine brain by flow cytometry.
Image caption:
Representative fluorograms obtained after flow cytometry analysis showing ROS, TNF-α, IL-1β, and IL-6 fluorescence. Samples of different brain regions from both hemispheres were analyzed in triplicate by flow cytometry (n = 6) for each animal and condition. Black lines correspond to auto-fluorescence, whereas grey areas correspond to positive cells. Sham, n = 6; HI+VEH, n = 6; HI+WIN, n = 6.
From:Alonso-Alconada D, Álvarez FJ, Goñi-de-Cerio F, Hilario E, Álvarez A.
Cannabinoid-mediated Modulation of Oxidative Stress and Early Inflammatory Response after Hypoxia-Ischemia.
Int J Mol Sci. 2020 Feb 14;21(4):1283.
doi: 10.3390/ijms21041283.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep Interleukin-6 antibody, clone 4B6 (MCA1659) used to evaluate IL-6 expression in ovine brain by immunogluorescence.
Image caption:
Representative microphotographs 3 h after HI TNF-α (a,b,c), IL-1β (d,e,f), and IL-6 (g,h,i) positive cells after immunofluorescence labeling. Cortex brain sections were obtained after sham operation (a,d,g), HI+vehicle (b,e,h) or HI+WIN treatment (c,f,i). The histological evaluation reveals the presence of these cytokines (arrows) in asphyctic animals. Original magnification: ×200.
From:Alonso-Alconada D, Álvarez FJ, Goñi-de-Cerio F, Hilario E, Álvarez A.
Cannabinoid-mediated Modulation of Oxidative Stress and Early Inflammatory Response after Hypoxia-Ischemia.
Int J Mol Sci. 2020 Feb 14;21(4):1283.
doi: 10.3390/ijms21041283.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep Interleukin-6
- Product Type
- Monoclonal Antibody
- Clone
- 4B6
- Isotype
- IgG1
- Specificity
- IL-6
Antibody Quality and Performance Guarantee
| Mouse anti Sheep Interleukin-6 antibody, clone 4B6 recognizes ovine interleukin-6 (IL-6) and has also been reported to recognise recombinant human IL-6 and bovine IL-6 transfected cells. Mouse anti Sheep Interleukin-6 antibody, clone 4B6 does not cross react with ovine IL-1 beta, IL-8, MCP-1 or TNF alpha. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- Recombinant ovine IL-6.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml (as a coating antibody) | |
| Flow Cytometry 1 | |
||
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
- ELISA
- Mouse anti Sheep interleukin-6 antibody, clone 4B6 may be used in combination with AHP424 in a sandwich ELISA assays for ovine IL-6.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
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| Description | Mouse IgG1 Negative Control | ||||||
References for IL-6 antibody
-
McWaters, P. et al. (2000) Characterisation of monoclonal antibodies to ovine interleukin-6 and the development of a sensitive capture ELISA.
Vet Immunol Immunopathol. 73 (2): 155-65. -
Shashikant, B.N. et al. (2005) Dose response to rhCC10-augmented surfactant therapy in a lamb model of infant respiratory distress syndrome: physiological, inflammatory, and kinetic profiles.
J Appl Physiol. 99: 2204-11. -
Kabaroff, L. et al. (2006) Changes in ovine maternal temperature, and serum cortisol and interleukin-6 concentrations after challenge with Escherichia coli lipopolysaccharide during pregnancy and early lactation.
J Anim Sci. 84: 2083-8. -
Su, F. et al (2007) Beneficial effects of ethyl pyruvate in septic shock from peritonitis.
Arch Surg.142: 166-71. -
Wang, Z. et al. (2008) Acute hypercapnia improves indices of tissue oxygenation more than dobutamine in septic shock.
Am J Respir Crit Care Med. 177: 178-83. -
Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48. -
Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
Reprod Sci. 22 (11): 1409-20. -
Karisnan, K .et al. (2015) Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
PLoS One. 10 (4): e0124390.
View The Latest Product References
-
Dooley, L.M. et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
PLoS One. 10 (5): e0124144. -
Herry, C.L. et al. (2016) Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.
PLoS One. 11 (4): e0153515. -
Ciliberti, M.G. et al. (2017) Peripheral blood mononuclear cell proliferation and cytokine production in sheep as affected by cortisol level and duration of stress.
J Dairy Sci. 100 (1): 750-756. -
Westover, A. et al. (2017) Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.
Front Physiol. 8: 871. -
Castel, A. et al. (2021) Recording and manipulation of vagus nerve electrical activity in chronically instrumented unanesthetized near term fetal sheep.
J Neurosci Methods. 360: 109257. -
Damiano, S. et al. (2022) Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambs
It J anim Sci 21 (1): 934-42. -
Ciliberti, M.G. et al. (2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model.
J Dairy Sci. 105 (5): 4335-53. -
Ferreras-Colino, E. et al. (2023) Oral immunization with heat-inactivated Mycobacterium bovis reduces local parasite dissemination and hepatic granuloma development in mice infected with Leishmania amazonensis.
Res Vet Sci. 162: 104963. -
Cao, M. et al. (2024) The Vagus Nerve Regulates Immunometabolic Homeostasis in the Ovine Fetus near Term: The Impact on Terminal Ileum.
Biology (Basel). 13 (1): 38. -
Ciliberti, M.G. et al. (2024) Role of hazelnut skin supplementation on plasma antioxidant status and cytokine profile in growing lambs.
Front Vet Sci. 11: 1340141. -
Alonso-Alconada, D. et al. (2020) Cannabinoid-mediated Modulation of Oxidative Stress and Early Inflammatory Response after Hypoxia-Ischemia.
Int J Mol Sci. 21 (4): 1283.
- RRID
- AB_322149
- UniProt
- P29455
- Entrez Gene
- IL6
- GO Terms
- GO:0006953 acute-phase response
- GO:0005125 cytokine activity
- GO:0005138 interleukin-6 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
MCA1659
148516
155912
160211
160994
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