IL-6 antibody | 4B6

Mouse anti Sheep Interleukin-6

Product Type
Monoclonal Antibody
Clone
4B6
Isotype
IgG1
Specificity
IL-6

Product Code Applications Pack Size List Price Your Price Qty
MCA1659
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SDS Safety Datasheet SDS
E F * WB 0.25 mg loader
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Mouse anti Sheep Interleukin-6 antibody, clone 4B6 recognizes ovine interleukin-6 (IL-6) and has also been reported to recognise recombinant human IL-6 and bovine IL-6 transfected cells. Mouse anti Sheep Interleukin-6 antibody, clone 4B6 does not cross react with ovine IL-1 beta, IL-8, MCP-1 or TNF alpha.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant ovine IL-6.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml (as a coating antibody)
Flow Cytometry 1
Western Blotting
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
ELISA
MCA1659 may also be used in combination with AHP424 in a sandwich ELISA assays for ovine IL-6.

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References for IL-6 antibody

  1. McWaters, P. et al. (2000) Characterisation of monoclonal antibodies to ovine interleukin-6 and the development of a sensitive capture ELISA.
    Vet Immunol Immunopathol. 73 (2): 155-65.
  2. Wang, Z. et al. (2008) Acute hypercapnia improves indices of tissue oxygenation more than dobutamine in septic shock.
    Am J Respir Crit Care Med. 177: 178-83.
  3. Su, F. et al (2007) Beneficial effects of ethyl pyruvate in septic shock from peritonitis.
    Arch Surg.142: 166-71.
  4. Kabaroff, L. et al. (2006) Changes in ovine maternal temperature, and serum cortisol and interleukin-6 concentrations after challenge with Escherichia coli lipopolysaccharide during pregnancy and early lactation.
    J Anim Sci. 84: 2083-8.
  5. Shashikant, B.N. et al. (2005) Dose response to rhCC10-augmented surfactant therapy in a lamb model of infant respiratory distress syndrome: physiological, inflammatory, and kinetic profiles.
    J Appl Physiol. 99: 2204-11.
  6. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  7. Karisnan, K .et al. (2015) Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
    PLoS One. 10 (4): e0124390.
  8. Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
    Reprod Sci. 22 (11): 1409-20.
  9. View The Latest Product References
  10. Dooley, L.M. et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
    PLoS One. 10 (5): e0124144.
  11. Herry, C.L. et al. (2016) Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.
    PLoS One. 11 (4): e0153515.
  12. Ciliberti, M.G. et al. (2017) Peripheral blood mononuclear cell proliferation and cytokine production in sheep as affected by cortisol level and duration of stress.
    J Dairy Sci. 100 (1): 750-756.
  13. Westover, A. et al. (2017) Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.
    Front Physiol. 8: 871.
  14. Castel, A. et al. (2021) Recording and manipulation of vagus nerve electrical activity in chronically instrumented unanesthetized near term fetal sheep.
    J Neurosci Methods. 360: 109257.
  15. Ciliberti, M.G. et al. (2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro. sheep model.
    J Dairy Sci. Mar 17 [Epub ahead of print].
  16. Cao, M. et al. (2022) The vagus nerve regulates immunometabolic homeostasis in the ovine fetus near term: impact on terminal ileum
    asXiv 2203.14967

RRID
AB_322149
UniProt
P29455
Entrez Gene
IL6
GO Terms
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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