MHC Class I Monomorphic antibody | 73-2
Mouse anti Rabbit MHC class I monomorphic, clone 73-2 (MCA810GA) used to assess MHC Class-1 expression in native and vacuum‐assisted decellularized trachea (VADT) tracheal grafts in rabbits by immunofluorescence.
Image caption:
Immunological evaluation of native and VADT grafts. (b) Immunofluorescence staining of DAPI/Col‐II (G, H), DAPI/MHC‐I (I, J), and DAPI/MHC‐II (K, L) expressed in the native trachea and VADT scaffolds.
From: Sun F, Shen Z, Zhang B, Lu Y, Shan Y, Wu Q, Yuan L, Zhu J, Pan S, Wang Z, Wu C, Zhang G, Yang W, Xu X, Shi H.
Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step.
Bioeng Transl Med. 2023 May 3;8(4):e10534.
doi: 10.1002/btm2.10534..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit MHC Class I Monomorphic
- Product Type
- Monoclonal Antibody
- Clone
- 73-2
- Isotype
- IgG1
- Specificity
- MHC Class I Monomorphic
Antibody Quality and Performance Guarantee
| Mouse anti Rabbit MHC class I monomorphic, clone 73-2, recognizes the lapine homologue of the human major histocompatibility complex (MHC) class Ib, a protein expressed at varying levels on the cell surface of the majority of nucleated rabbit cells. MHC genes are found in all vertebrate species. The nomenclature varies for different species, often collectively named as leukocyte antigens and prefixed by the species or species group, e.g. human leukocyte antigen (HLA), bovine leukocyte antigen (BoLA) etc. Rabbit major histocompatibility complex molecules are generally referred to as MHC, however some sources have referenced them as Rabbit leukocyte antigens (RLA). MHC molecules comprise a vital component of adaptive immunity, MHC class I molecules are glycoproteins that function in the presentation of antigen fragments from antigen processing cells to cytotoxic T cells. Mouse anti Rabbit MHC class I monomorphic, clone 73-2 is specific for the rabbit MHC class Ib and provides a useful flow cytometry marker of rabbit nucleated cells. |
- Target Species
- Rabbit
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Rabbit T cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1:50 | 1:200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) | STAR117D405GA | F IF | 0.1 mg |
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| Description | Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) | ||||||
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Rabbit CD8:FITC | MCA1576F | F | 0.1 mg |
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| Description | Mouse anti Rabbit CD8:FITC | ||||||
| Mouse anti Rabbit CD4:FITC | MCA799F | F | 100 Tests |
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| Description | Mouse anti Rabbit CD4:FITC | ||||||
| Mouse anti Rabbit CD45 | MCA808GA | C F IF | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Rabbit CD45 | ||||||
| Mouse anti Rabbit MHC Class II DQ | MCA811GA | C F IP | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Rabbit MHC Class II DQ | ||||||
| Mouse anti Rabbit IgM (B Cell Marker):FITC | MCA812F | F | 0.1 mg |
|
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| List Price | Your Price | ||||||
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| Description | Mouse anti Rabbit IgM (B Cell Marker):FITC | ||||||
| Mouse anti Rabbit IgM (B Cell Marker):RPE | MCA812PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Rabbit IgM (B Cell Marker):RPE | ||||||
References for MHC Class I Monomorphic antibody
-
Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
Biotechnol Prog. 26 (6): 1749-58. -
Sun, F. et al. (2023) Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step.
Bioeng Transl Med. 8 (4): e10534.
Further Reading
-
Sakai, A. et al. (1997) P-selectin and vascular cell adhesion molecule-1 are focally expressed in aortas of hypercholesterolemic rabbits before intimal accumulation of macrophages and T lymphocytes.
Arterioscler Thromb Vasc Biol. 17: 310-6.
MCA810GA
0715
168617
169421
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