MHC Class I Monomorphic antibody | 73-2

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Mouse anti Rabbit MHC Class I Monomorphic

Product Type
Monoclonal Antibody
Clone
73-2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA810GA F 0.1 mg
Mouse anti Rabbit MHC class I monomorphic, clone 73-2, recognizes the lapine homologue of the human major histocompatibility complex (MHC) class Ib, a protein expressed at varying levels on the cell surface of the majority of nucleated rabbit cells.

MHC genes are found in all vertebrate species. The nomenclature varies for different species, often collectively named as leukocyte antigens and prefixed by the species or species group, e.g. human leukocyte antigen (HLA), bovine leukocyte antigen (BoLA) etc. Rabbit major histocompatibility complex molecules are generally referred to as MHC, however some sources have referenced them as Rabbit leukocyte antigens (RLA). MHC molecules comprise a vital component of adaptive immunity, MHC class I molecules are glycoproteins that function in the presentation of antigen fragments from antigen processing cells to cytotoxic T cells.

Mouse anti Rabbit MHC class I monomorphic, clone 73-2 is specific for the rabbit MHC class Ib and provides a useful flow cytometry marker of rabbit nucleated cells.

Product Details

Target Species
Rabbit
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Rabbit T cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of MHC Class I Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1:50 1:200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) STAR117D405GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Rabbit CD8:FITC MCA1576F 0.1 mg F
Mouse anti Rabbit CD4:FITC MCA799F 100 Tests F
Mouse anti Rabbit CD45 MCA808GA 0.1 mg C F IF
Mouse anti Rabbit MHC Class II DQ MCA811GA 0.1 mg C F IP
Mouse anti Rabbit IgM (B Cell Marker):FITC MCA812F 0.1 mg F
Mouse anti Rabbit IgM (B Cell Marker):RPE MCA812PE 100 Tests F

Product Specific References

References for MHC Class I Monomorphic antibody

  1. Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
    Biotechnol Prog. 26 (6): 1749-58.

Further Reading

  1. Sakai, A. et al. (1997) P-selectin and vascular cell adhesion molecule-1 are focally expressed in aortas of hypercholesterolemic rabbits before intimal accumulation of macrophages and T lymphocytes.
    Arterioscler Thromb Vasc Biol. 17: 310-6.