Mouse anti Rabbit MHC class I monomorphic, clone 73-2, recognizes the lapine homologue of the human major histocompatibility complex (MHC) class Ib, a protein expressed at varying levels on the cell surface of the majority of nucleated rabbit cells.
MHC genes are found in all vertebrate species. The nomenclature varies for different species, often collectively named as leukocyte antigens and prefixed by the species or species group, e.g. human leukocyte antigen (HLA), bovine leukocyte antigen (BoLA) etc. Rabbit major histocompatibility complex molecules are generally referred to as MHC, however some sources have referenced them as Rabbit leukocyte antigens (RLA). MHC molecules comprise a vital component of adaptive immunity, MHC class I molecules are glycoproteins that function in the presentation of antigen fragments from antigen processing cells to cytotoxic T cells.
Mouse anti Rabbit MHC class I monomorphic, clone 73-2 is specific for the rabbit MHC class Ib and provides a useful flow cytometry marker of rabbit nucleated cells.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Rabbit T cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MHC Class I Monomorphic antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for MHC Class I Monomorphic antibody
Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
Biotechnol Prog. 26 (6): 1749-58.
Sakai, A. et al. (1997) P-selectin and vascular cell adhesion molecule-1 are focally expressed in aortas of hypercholesterolemic rabbits before intimal accumulation of macrophages and T lymphocytes.
Arterioscler Thromb Vasc Biol. 17: 310-6.
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