CD8 antibody | 12.C7

Mouse anti Rabbit CD8:FITC

Product Type
Monoclonal Antibody
Clone
12.C7
Isotype
IgG1
Specificity
CD8

Product Code Applications Pack Size List Price Your Price Qty
MCA1576F
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F 0.1 mg loader
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Mouse anti Rabbit CD8 antibody, clone 12.C7 recognizes the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity.

Target Species
Rabbit
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

References for CD8 antibody

  1. Civin, C.I. et al. (1984) Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.
    J Immunol. 133 (1): 157-65.
  2. De Smet, W. et al. (1983) Rabbit leukocyte surface antigens defined by monoclonal antibodies.
    Eur J Immunol. 13: 919-28.
  3. Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
    PLos ONE 3: e1627.
  4. Zhao, L. et al. (2011) Evaluation of immunocompatibility of tissue-engineered periosteum.
    Biomed Mater.6:015005.
  5. Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
    Immunology. 77: 106-8.
  6. Marques, R.M. et al. (2012) Early inflammatory response of young rabbits attending natural resistance to calicivirus (RHDV) infection.
    Vet Immunol Immunopathol. 150: 181-8.
  7. Schock, A. and Reid, H.W. (1996) Characterisation of the lymphoproliferation in rabbits experimentally affected with malignant catarrhal fever.
    Vet Microbiol. 53: 111-9.
  8. Anderson, I.E. et al. (2008) Production and utilization of interleukin-15 in malignant catarrhal fever.
    J Comp Pathol. 138 (2-3): 131-44.
  9. View The Latest Product References
  10. Khan AA et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
    J Virol. 89 (13): 6619-32.
  11. Srivastava, R. et al. (2015) A Herpes Simplex Virus Type 1 Human Asymptomatic CD8+ T-Cell Epitopes-Based Vaccine Protects Against Ocular Herpes in a "Humanized" HLA Transgenic Rabbit Model.
    Invest Ophthalmol Vis Sci. 56 (6): 4013-28.
  12. Hanson, N.B. & Lanning, D.K. (2008) Microbial induction of B and T cell areas in rabbit appendix.
    Dev Comp Immunol. 32 (8): 980-91.
  13. Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
    J Leukoc Biol. 85 (1): 44-54.
  14. Dewals, B. et al. (2011) Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever.
    J Virol. 85 (14): 6941-54.
  15. Khan, A.A. et al. (2018) Human Asymptomatic Epitope Peptide/CXCL10-Based Prime/Pull Vaccine Induces Herpes Simplex Virus-Specific Gamma Interferon-Positive CD107+ CD8+ T Cells That Infiltrate the Corneas and Trigeminal Ganglia of Humanized HLA Transgenic Rabbits and Protect against Ocular Herpes Challenge.
    J Virol. 92 (16): e00535-18.
  16. Stich N et al. (2010) Staphylococcal superantigen (TSST-1) mutant analysis reveals that t cell activation is required for biological effects in the rabbit including the cytokine storm.
    Toxins (Basel). 2 (9): 2272-88.
  17. Myster, F. et al. (2015) Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
    J Virol. 89 (7): 3630-47.
  18. Srivastava, R. et al. (2016) The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
    J Virol. 90 (8): 3913-28.
  19. Gates, K.V. & Griffiths, L.G. (2018) Chronic graft-specific cell-mediated immune response toward candidate xenogeneic biomaterial.
    Immunol Res. 66 (2): 288-98.
  20. Prakash, S. et al. (2020) Unique molecular signatures of antiviral memory CD8+ T cells associated with asymptomatic recurrent ocular herpes.
    Sci Rep. 10 (1): 13843.
  21. Jeklova, E. et al. (2020) Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi..
    Vet Res. 51 (1): 79.
  22. Myster, F. et al. (2020) Alcelaphine herpesvirus 1 genes A7 and A8 regulate viral spread and are essential for malignant catarrhal fever.
    PLoS Pathog. 16 (3): e1008405.
  23. Niedźwiedzka-Rystwej, P. et al. (2020) B and T lymphocytes in rabbits change according to the sex and throughout the year.
    Pol J Vet Sci. 23 (1): 37-42.
  24. Niedźwiedzka-Rystwej, P. et al. (2021) Reactivity of selected markers of innate and adaptive immunity in rabbits experimentally infected with antigenic variants of RHD (Lagovirus europaeus/GI.1a).
    Vet Res Commun. Oct 29 [Epub ahead of print].

RRID
AB_566891
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