IgM antibody | NRBM
Mouse anti Rabbit IgM antibody, clone NRBM (MCA812GA) used to assess lapine splenocyte IgM expression following infection with Encephalitozoon cuniculi.
Image caption:
Representative flow cytometry analysis of CFSE labelled splenocytes in control uninfected rabbit (con) and rabbits in 2, 4, 6 and 8 weeks after E. cuniculioral infection. Splenocytes were stimulated with E. cuniculi spores and after 6 days of incubation stained with specific monoclonal antibodies against cell surface markers.
From: Jeklova E, Leva L, Matiasovic J, Ondrackova P, Kummer V, Faldyna M.
Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi..
Vet Res. 2020 Jun 15;51(1):79.
doi: 10.1186/s13567-020-00806-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Rabbit IgM (B Cell Marker):RPE
- Product Type
- Monoclonal Antibody
- Clone
- NRBM
- Isotype
- IgG1
- Specificity
- IgM
- Region
- (B-CELL MARKER)
Antibody Quality and Performance Guarantee
| Mouse anti Rabbit IgM (B Cell Marker) antibody, clone NRBM recognizes rabbit IgM. Mammalian IgM is produced and secreted by plasma cells located in bone marrow, lymph nodes and spleen. IgM is present in both a secreted polymeric form and as cell surface monomeric form on B cells. Mouse anti Rabbit IgM antibody, clone NRBM labels IgM+ve B cells (Dewals et al. 2011, Waclavicek et al. 2009) and as such can be considered a reliable marker of lagomorph B cells for flow cytometry. |
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Fusion Partners
- Spleen cells from immunised mice were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Rabbit T Lymphocytes:FITC | MCA800F | F | 100 Tests |
|
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| List Price | Your Price | ||||||
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| Description | Mouse anti Rabbit T Lymphocytes:FITC | ||||||
References for IgM antibody
-
Idogawa, H. et al. (1997) Progression of articular destruction and the production of tumour necrosis factor-alpha in antigen-induced arthritis in rabbits.
Scand J Immunol. 46 (6): 572-80. -
Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
PLoS One 3: e1627. -
Gillet, L. et al. (2009) Anchoring tick salivary anti-complement proteins IRAC I and IRAC II to membrane increases their immunogenicity.
Vet Res. 40: 51. -
Stich, N. et al. (2010) Staphylococcal superantigen (TSST-1) mutant analysis reveals that t cell activation is required for biological effects in the rabbit including the cytokine storm.
Toxins (Basel). 2 (9): 2272-88. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85 (1): 44-54. -
Anderson, I.E. et al. (2008) Production and utilization of interleukin-15 in malignant catarrhal fever.
J Comp Pathol. 138: 131-44. -
Dewals, B.G. and Vanderplasschen, A. (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 42: 95. -
Dewals, B. et al. (2011) Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever.
J Virol. 85: 6941-54.
View The Latest Product References
-
Milanovic, V. et al. (2017) Histological and immunological changes in uterus during the different reproductive stages at Californian rabbit (Oryctolagus cuniculus).
Kafkas Univ Vet Fak Derg, 23, 137-44. -
Ondruska, L. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine.
Veterinární Medicína. 61 (No. 10): 571-6. -
Myster, F. et al. (2015) Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
J Virol. 89 (7): 3630-47. -
Sorel, O. et al. (2017) Macavirus latency-associated protein evades immune detection through regulation of protein synthesis in cis depending upon its glycin/glutamate-rich domain.
PLoS Pathog. 13 (10): e1006691. -
Jeklova, E. et al. (2020) Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi..
Vet Res. 51 (1): 79. -
Niedżwiedzka-Rystwej, P. et al. (2020) B and T lymphocytes in rabbits change according to the sex and throughout the year.
Pol J Vet Sci. 23 (1): 37-42. -
Muñoz-Silvestre, A. et al. (2020) Pathogenesis of Intradermal Staphylococcal Infections: Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections.
Am J Pathol. 190 (6): 1188-210. -
Niedżwiedzka-Rystwej, P. et al. (2022) Reactivity of selected markers of innate and adaptive immunity in rabbits experimentally infected with antigenic variants of RHD (Lagovirus europaeus/GI.1a).
Vet Res Commun. 46 (1): 233-42.
- UniProt
- P04221
- P03988
- GO Terms
- GO:0003823 antigen binding
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
MCA812PE
160693
INN1808
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