CD4 antibody | KEN-4

Mouse anti Rabbit CD4:FITC

Product Type
Monoclonal Antibody
Clone
KEN-4
Isotype
IgG2a
Specificity
CD4

Product Code Applications Pack Size List Price Your Price Qty
MCA799F
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Mouse anti Rabbit CD4 antibody, clone KEN-4 recognizes the rabbit CD4 cell surface antigen, also known as T-cell surface antigen T4/Leu-3. Rabbit CD4 is a 434 amino acid, with an additional N-terminal signal peptide ~50 kDa cell surface single pass, type I transmembrane glycoprotein expressed by T helper cells.

Mouse anti Rabbit CD4 antibody, clone KEN-4 blocks the allogeneic mixed lymphocyte reaction response.

Target Species
Rabbit
Species Cross-Reactivity
Target SpeciesCross Reactivity
Brown Hare (Lepus europeus)
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Rabbit thymocytes.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse PAI myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
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Description Mouse IgG2a Negative Control:FITC

References for CD4 antibody

  1. Kotani, M. et al. (1993) Generation and characterization of monoclonal antibodies against rabbit CD4, CD5 and CD11a antigens.
    J Immunol Methods. 157 (1-2): 241-52.
  2. Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
    PLos ONE 3: e1627.
  3. Chentoufi, A.A. et al. (2010) A novel HLA (HLA-A*0201) transgenic rabbit model for preclinical evaluation of human CD8+ T cell epitope-based vaccines against ocular herpes.
    J Immunol. 184: 2561-71.
  4. Perosa, F. and Dammacco, F. (1994) Anti-idiotypic monoclonal antibodies (mAb) to an anti-CD4 mAb induce CD4+ T cell depletion in rabbit.
    Int J Clin Lab Res. 24: 208-12.
  5. Khan, A.A. et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
    J Virol. 89 (13): 6619-32.
  6. Rütgen, B.C. et al. (2014) Exploratory assessment of CD4+ T lymphocytes in brown hares (Lepus europeus) using a cross-reactive anti-rabbit CD4 antibody.
    Vet Immunol Immunopathol. 161 (1-2): 108-15.
  7. Boutard, B. et al. (2015) The α2,3-sialyltransferase encoded by myxoma virus is a virulence factor that contributes to immunosuppression.
    PLoS One. 10 (2): e0118806.
  8. Pakandl, M. et al. (2008) Dependence of the immune response to coccidiosis on the age of rabbit suckling.
    Parasitol Res. 103 (6): 1265-71.
  9. View The Latest Product References
  10. Renaux, S. et al. (2003) Dynamics and responsiveness of T-lymphocytes in secondary lymphoid organs of rabbits developing immunity to Eimeria intestinalis.
    Vet Parasitol. 110 (3-4): 181-95.
  11. Yang, J. et al. (2009) Expression and localization of rabbit B-cell activating factor (BAFF) and its specific receptor BR3 in cells and tissues of the rabbit immune system.
    Dev Comp Immunol. 33 (5): 697-708.
  12. Beghelli, D et al. (2016) Effects of Oregano (Origanum vulgare L.) and Rosemary (Rosmarinus officinalis L.) Aqueous Extracts On in vitro Rabbit Immune Responses
    MOJ Immunology. 4 (4) [Epub ahead of print].
  13. Sorel, O. et al. (2017) Macavirus latency-associated protein evades immune detection through regulation of protein synthesis in cis depending upon its glycin/glutamate-rich domain.
    PLoS Pathog. 13 (10): e1006691.
  14. Myster, F. et al. (2015) Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
    J Virol. 89 (7): 3630-47.
  15. Penadés, M. et al. (2018) Long-term implications of feed energy source in different genetic types of reproductive rabbit females. II. Immunologic status.
    Animal. 12 (9): 1877-85.
  16. Jeklova, E. et al. (2020) Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi..
    Vet Res. 51 (1): 79.
  17. Niedźwiedzka-Rystwej, P. et al. (2020) B and T lymphocytes in rabbits change according to the sex and throughout the year.
    Pol J Vet Sci. 23 (1): 37-42.
  18. Muñoz-Silvestre, A. et al. (2020) Pathogenesis of Intradermal Staphylococcal Infections: Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections.
    Am J Pathol. 190 (6): 1188-1210.
  19. Largo, R.D. et al. (2020) VEGF Over-Expression by Engineered BMSC Accelerates Functional Perfusion, Improving Tissue Density and In-Growth in Clinical-Size Osteogenic Grafts.
    Front Bioeng Biotechnol. 8: 755.
  20. Niedźwiedzka-Rystwej, P. et al. (2021) Reactivity of selected markers of innate and adaptive immunity in rabbits experimentally infected with antigenic variants of RHD (Lagovirus europaeus/GI.1a).
    Vet Res Commun. Oct 29 [Epub ahead of print].
  21. Parameswaran, N. et al. (2014) The A2 gene of alcelaphine herpesvirus-1 is a transcriptional regulator affecting cytotoxicity in virus-infected T cells but is not required for malignant catarrhal fever induction in rabbits.
    Virus Res. 188: 68-80.

Flow Cytometry

RRID
AB_2075555
UniProt
P46630
Entrez Gene
CD4
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0045058 T cell selection
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