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CD8 Beta antibody | PPT23

Mouse anti Pig CD8 Beta Chain:RPE

Product Type
Monoclonal Antibody
CD8 Beta

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price

Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.

Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine α/β T cells, in thymic tissue both CD8lo and CD8hi cells are recognized (Yang & Parkhouse 1997). Mouse anti Pig CD8 beta chain, clone PPT23 recognizes a different epitope on the CD8 beta chain to clone PPT22.

Target Species
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitute with 1 ml distilled water
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Porcine thymus membrane lysate
Fusion Partners
Lymph node cells from immunized BALB/c mice were fused with cells of the NS0 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
For research purposes only
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Pig CD4 Alpha MCA1749GA C * F 0.1 mg loader
List Price Your Price
Description Mouse anti Pig CD4 Alpha
Mouse anti Pig CD45RA:FITC MCA1751F F 0.1 mg loader
List Price Your Price
Description Mouse anti Pig CD45RA:FITC
Mouse anti Pig CD3:FITC MCA5951F F 0.1 mg loader
List Price Your Price
Description Mouse anti Pig CD3:FITC
Mouse anti Pig CD3 MCA5951GA C F IP WB 0.1 mg loader
List Price Your Price
Description Mouse anti Pig CD3

Source Reference

  1. Yang, H. & Parkhouse, R.M. (1997) Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets.
    Immunology. 92 (1): 45-52.

References for CD8 Beta antibody

  1. Sinkora, M. et al. (2007) Two groups of porcine TCRgammadelta+ thymocytes behave and diverge differently.
    J Immunol. 178 (2): 711-9.
  2. Mair, K.H. et al. (2015) Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS® MLV.
    Vet Microbiol. 176 (3-4): 352-7.

Further Reading

  1. Zuckermann, F.A. et al. (1998) Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop.
    Vet Immunol Immunopathol. 60 (3-4): 291-303.



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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