CD8 Beta antibody | PPT23
Filter by Application:
F ResetMouse anti Pig CD8 Beta Chain:RPE
- Product Type
- Monoclonal Antibody
- Clone
- PPT23
- Isotype
- IgG1
- Specificity
- CD8 Beta
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen. Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine α/β T cells, in thymic tissue both CD8lo and CD8hi cells are recognized (Yang & Parkhouse 1997). Mouse anti Pig CD8 beta chain, clone PPT23 recognizes a different epitope on the CD8 beta chain to clone PPT22. |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- Porcine thymus membrane lysate
- Fusion Partners
- Lymph node cells from immunized BALB/c mice were fused with cells of the NSO myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
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| Description | Mouse IgG1 Negative Control:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Mouse anti Pig CD4 Alpha | MCA1749GA | C* F | 0.1 mg |
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| Description | Mouse anti Pig CD4 Alpha | ||||||
| Mouse anti Pig CD45RA:FITC | MCA1751F | F | 0.1 mg |
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| Description | Mouse anti Pig CD45RA:FITC | ||||||
| Mouse anti Pig CD3:FITC | MCA5951F | F | 0.1 mg |
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| Description | Mouse anti Pig CD3:FITC | ||||||
| Mouse anti Pig CD3 | MCA5951GA | C F IP WB | 0.1 mg |
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| Description | Mouse anti Pig CD3 | ||||||
References for CD8 Beta antibody
-
Yang, H. & Parkhouse, R.M. (1997) Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets.
Immunology. 92 (1): 45-52. -
Mair, K.H. et al. (2015) Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS® MLV.
Vet Microbiol. 176 (3-4): 352-7. -
Sinkora, M. et al. (2007) Two groups of porcine TCRgammadelta+ thymocytes behave and diverge differently.
J Immunol. 178 (2): 711-9.
Further Reading
-
Zuckermann, F.A. et al. (1998) Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop.
Vet Immunol Immunopathol. 60 (3-4): 291-303.
MCA5954PE
INN1114
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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