CD45RA antibody | MIL13
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|Mouse anti Pig CD45RA, clone MIL13, recognizes an epitope contained in the portion of porcine CD45 encoded by exon A, CD45RA (Lunney et al. 2007).
Mouse anti pig CD45RA, clone MIL13 recognizes both the 210 kDa RA CD45 isoform and the 226 kDa RAC isoform (Zuckermann et al. 2001). Clone MIL13 does not recognize the CD45RC or CD45RO isoforms.
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin
- Cells isolated from porcine mesenteric lymph node
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
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- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD45RA antibody
Bozić F et al. (2002) Recruitment of intestinal CD45RA+ and CD45RC+ cells induced by a candidate oral vaccine against porcine post-weaning colibacillosis.
Vet Immunol Immunopathol. 86 (3-4): 137-46.
Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
Vet Res Commun. 26: 273-83.
Pakkanen, T.M. et al. (2000) Periadventitial lacZ gene transfer to pig carotid arteries using a biodegradable collagen collar or a wrap of collagen sheet with adenoviruses and plasmid-liposome complexes.
J Gene Med. 2: 52-60.
Schierack, P. et al. (2009) Effects of Bacillus cereus var. toyoi on immune parameters of pregnant sows.
Vet Immunol Immunopathol. 127: 26-37.
Suzuki, S. et al. (2016) Generation and characterization of RAG2 knockout pigs as animal model for severe combined immunodeficiency.
Vet Immunol Immunopathol. 178: 37-49.
López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
BMC Vet Res. 15 (1): 91.
Li, K.et al. (2019) Generation of porcine monoclonal antibodies based on single cell technologies.
Vet Immunol Immunopathol. 215: 109913.
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366.
View The Latest Product References
Ogihara, K. et al. (2022) A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA.
J Vet Med Sci. Apr 11 [Epub ahead of print].
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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