CD8 Beta antibody | PPT23

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Mouse anti Pig CD8 Beta Chain:FITC

Mouse anti Pig CD8 Beta Chain

Mouse anti Pig CD8 Beta Chain:RPE

Product Type
Monoclonal Antibody
Clone
PPT23
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA5954F
Datasheet
F
MSDS
0.1 mg loader

MCA5954GA
Datasheet
F
MSDS
0.1 mg loader

MCA5954PE
Datasheet
F
MSDS
100 Tests loader

Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.

Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine α/β T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22.

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Porcine thymus membrane lysate
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Lymph node cells from immunized BALB/c mice were fused with cells of the NSO myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
18 months from date of despatch
Guarantee
18 months from date of despatch
Guarantee
12 months from date of reconstitution

More Information

Regulatory
For research purposes only

Applications of CD8 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/200
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Pig CD4 Alpha MCA1749GA C* F 0.1 mg loader
Mouse anti Pig CD4 Alpha:RPE MCA1749PE F 100 Tests loader
Mouse anti Pig CD3 MCA5951GA C F IP WB 0.1 mg loader
Mouse anti Pig CD3:RPE MCA5951PE F 100 Tests loader
Mouse anti Pig wCD8 Alpha:FITC MCA1223F F 0.1 mg loader
Mouse anti Pig wCD8 Alpha MCA1223GA C F 0.1 mg loader
Mouse anti Pig wCD8 Alpha:RPE MCA1223PE F 100 Tests loader
Mouse anti Pig CD4 Alpha:FITC MCA1749F F 0.1 mg loader
Mouse anti Pig CD4 Alpha MCA1749GA C* F 0.1 mg loader
Mouse anti Pig CD4 Alpha:RPE MCA1749PE F 100 Tests loader
Mouse anti Pig CD5:FITC MCA2307F F IP WB 0.1 mg loader
Mouse anti Pig CD3 MCA5951GA C F IP WB 0.1 mg loader
Mouse anti Pig CD4 Alpha MCA1749GA C* F 0.1 mg loader
Mouse anti Pig CD45RA:FITC MCA1751F F 0.1 mg loader
Mouse anti Pig CD3:FITC MCA5951F F 0.1 mg loader
Mouse anti Pig CD3 MCA5951GA C F IP WB 0.1 mg loader

Product Specific References

References for CD8 Beta antibody

  1. Yang, H. & Parkhouse, R.M. (1997) Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets.
    Immunology. 92 (1): 45-52.
  2. Mair, K.H. et al. (2015) Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS® MLV.
    Vet Microbiol. 176 (3-4): 352-7.
  3. Sinkora, M. et al. (2007) Two groups of porcine TCRgammadelta+ thymocytes behave and diverge differently.
    J Immunol. 178 (2): 711-9.

Further Reading

  1. Zuckermann, F.A. et al. (1998) Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop.
    Vet Immunol Immunopathol. 60 (3-4): 291-303.