CD44 antibody | MAC329

Product Code Applications Pack Size List Price Your Price Qty
MCA1449GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Pig CD44 antibody, clone MAC329 recognizes the porcine CD44 cell surface antigen which is involved in cell-cell interactions, cell adhesion and migration. CD44 is also a receptor for hyaluronic acid and can interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs).

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Horse
Bovine
Goat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified porcine CD44
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised LOU Rats were fused with cells of the Y3.Ag1.2.3 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Rat IgG1 Negative Control

References for CD44 antibody

  1. Yang, H. et al. (1993) Preparation and reactivities of anti-porcine CD44 monoclonal antibodies.
    Scand J Immunol. 37 (4): 490-8.
  2. Kasper, M. et al. (1996) Expression of CD44 isoforms during bleomycin-or radiation-induced pulmonary fibrosis in rats and mini-pigs.
    Histochem Cell Biol. 105 (3): 221-30.
  3. Gyongyosi, M. et al. (2010) Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.
    Thromb Haemost. 103: 1-9.
  4. Smith, M.M. et al. (2008) Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy.
    Rheumatology (Oxford). 47:1172-8.
  5. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
    Am J Trop Med Hyg. 70: 618-24.
  6. Lacroux, C. et al. (2012) Study of canine cutaneous melanocytic tumours: evaluation of histological and immunohistochemical prognostic criteria in 65 cases
    Revue de Médecine Vétérinaire 163: 393-401.
  7. Zimmermann, C.E. et al. (2021) Characterization of porcine mesenchymal stromal cells and their proliferative and osteogenic potential in long-term culture.
    J Stem Cells Regen Med. 17 (2): 49-55.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Immunohistology - Paraffin

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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