CD4 antibody | b38c6c7

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MCA6045GA
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F 0.1 mg loader
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Mouse anti Porcine CD4, clone b38c6c7 recognizes a ~55 kDa porcine homologue of the human CD4 antigen found on the surface of T helper cells. Clone b38c6c7 was confirmed to bind to a member of the CD4 alpha cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haverson et al. 2001).

Porcine CD4 is a type 1 transmembrane member of the immunoglobulin superfamily. Pigs are unusual among mammalian species as they appear to have four populations of resting T lymphocytes. In addition to CD4+/CD8- and CD4-/CD8+ lymphocytes they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but not in the case of mature peripheral T cells (Saalmuller et al. 1987).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine leukocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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References for CD4 antibody

  1. Saalmüller A et al. (2001) Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies.
    Vet Immunol Immunopathol. 80 (1-2): 35-52.
  2. Gabner, S.,et al. (2014) Immune response to Cystoisospora suis in piglets: local and systemic changes in T-cell subsets and selected mRNA transcripts in the small intestine.
    Parasite Immunology 36: 277–291
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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