Natalizumab antibody | AbD21375
Human anti Natalizumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD21375
- Isotype
- HuCAL Fab monovalent
- Specificity
- Natalizumab
Human Anti-Natalizumab Antibody, clone AbD21375 is a paratope specific, anti-idiotypic antibody that specifically recognizes free natalizumab but not free human alpha-4/beta-1 integrin or the drug/integrin complex. It inhibits the binding of natalizumab to its target and can be used to measure levels of free natalizumab and biosimilar products in bioanalytical and patient monitoring assays. A pair of anti-natalizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as a capture antibody, paired with an HRP conjugated Anti-Natalizumab Antibody in full immunoglobulin format, clone AbD21375_hlgG1 (HCA249) as the detection antibody. Natalizumab (Tysabri) is a humanized IgG4/kappa monoclonal antibody approved as a monotherapy for the treatment of relapsing forms of multiple sclerosis and for the treatment of Crohn’s disease. It is directed against alpha-4 integrin (CD49d), and binds to alpha-4/beta-1 integrin on the surface of circulating leucocytes, except neutrophils. It is believed to act by blocking the interaction of alpha-4/beta-1 integrin with vascular cell adhesion molecule-1 (VCAM-1), preventing the adherence and transport of inflammatory immune cells across the intestinal lining and blood-brain barrier. View a summary of all anti-natalizumab antibodies |
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Natalizumab
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD = 2.1 nM as measured by real time, label free molecular interaction analysis on immobilized natalizumab.
- Approx. Protein Concentrations
- HCA248G: Antibody concentration 1.0 mg/ml
- HCA248: Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
-
HCA248G
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Tysabri® is a registered trademark Biogen Idec and Elan.
His-tag is a registered trademark of EMD Biosciences. -
HCA248
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Tysabri is a trademark Biogen Idec and Elan.
His-tag is a trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in a either a direct ELISA or indirect ELISA both as a capture reagent and as a detection reagent.
Protocol: PK bridging ELISA to measure free drug.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
Human anti Natalizumab | HCA249 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Natalizumab | ||||||
Human anti Natalizumab | HCA250 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Natalizumab | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
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Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Rat anti DYKDDDDK Tag:HRP | ||||||
Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Penta Histidine Tag:HRP |
- Synonyms
- TYSABRI®
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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