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Natalizumab antibody | AbD21375

Human anti Natalizumab

Product Type
Monoclonal Antibody
Clone
AbD21375
Isotype
HuCAL Fab monovalent
Specificity
Natalizumab

Product Code Applications Pack Size List Price Your Price Qty
HCA248G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
List Price Your Price
loader
HCA248
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Natalizumab Antibody, clone AbD21375 is a paratope specific, anti-idiotypic antibody that specifically recognizes free natalizumab but not free human alpha-4/beta-1 integrin or the drug/integrin complex. It inhibits the binding of natalizumab to its target and can be used to measure levels of free natalizumab and biosimilar products in bioanalytical and patient monitoring assays.

A pair of anti-natalizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as a capture antibody, paired with an HRP conjugated Anti-Natalizumab Antibody in full immunoglobulin format, clone AbD21375_hlgG1 (HCA249) as the detection antibody.

Natalizumab (Tysabri) is a humanized IgG4/kappa monoclonal antibody approved as a monotherapy for the treatment of relapsing forms of multiple sclerosis and for the treatment of Crohn’s disease. It is directed against alpha-4 integrin (CD49d), and binds to alpha-4/beta-1 integrin on the surface of circulating leucocytes, except neutrophils. It is believed to act by blocking the interaction of alpha-4/beta-1 integrin with vascular cell adhesion molecule-1 (VCAM-1), preventing the adherence and transport of inflammatory immune cells across the intestinal lining and blood-brain barrier.

View a summary of all anti-natalizumab antibodies
20th Anniversary

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Natalizumab
Affinity
The intrinsic affinity of the monovalent form of this antibody is KD = 2.1 nM as measured by real time, label free molecular interaction analysis on immobilized natalizumab.
Approx. Protein Concentrations
HCA248G: Antibody concentration 1.0 mg/ml
HCA248: Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
HCA248G
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Tysabri® is a registered trademark Biogen Idec and Elan.
His-tag is a registered trademark of EMD Biosciences.
HCA248
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Tysabri is a trademark Biogen Idec and Elan.
His-tag is a trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product may be used in a either a direct ELISA or indirect ELISA both as a capture reagent and as a detection reagent.
Protocol: PK bridging ELISA to measure free drug.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Natalizumab HCA249 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Natalizumab
Human anti Natalizumab HCA250 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Natalizumab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
Rat anti DYKDDDDK Tag:HRP MCA4764P E P WB 0.1 mg
List Price Your Price
Description Rat anti DYKDDDDK Tag:HRP
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
List Price Your Price
loader
Description Mouse anti Penta Histidine Tag:HRP

Synonyms
TYSABRI®

HCA248G

150367 157731 163720

HCA248

148213 157721 164799

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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