TIM-4 antibody | RMT4-54
Filter by Application:F Reset
Rat anti Mouse TIM-4
- Product Type
- Monoclonal Antibody
|Rat anti Mouse TIM-4 antibody, clone RMT4-54 recognizes mouse Tim-4 (T-cell immunoglobulin mucin 4), a transmembrane glycoprotein and member of the immunoglobulin superfamily, exclusively expressed by antigen-presenting cells, which acts as the natural ligand of Tim-1, involved in T-cell expansion and cytokine production.
Tim-4 is necessary for the phagocytosis of apoptotic cells by peritoneal macrophages (Nishi et al. 2014) and interaction between Tim-1 on Th2 cells and Tim-4 on DCs, enhances Th2 cell function. The TIM-4 gene locus is associated with predisposition to asthma in both mice and humans and may be considered as a candidate disease gene for asthma.
Rat anti Mouse TIM-4 antibody, clone RMT4-54 inhibits the efficient in vitro phagocytosis of apoptotic cells by resident peritoneal Mac1+ cells), but not inflammatory peritoneal exudate Mac1+ cells (Nakayama et al. 2009).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Tim-4-Ig, consisting of amino acids 1-288 of mouse Tim-4, coupled with the Fc portion of mouse IgG2a.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Lymph node cells from immunised SD rats were fused with cells of the P3U1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Mouse spleen
Nakayama, M. et al. (2009) Tim-3 mediates phagocytosis of apoptotic cells and cross-presentation.
Blood. 113 (16): 3821-30.
Freeman, G.J. et al. (2010) TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.
Immunol Rev. 235 (1): 172-89.
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.Yes, sign me up