TIM-4 antibody | RMT4-54
Rat anti Mouse TIM-4
- Product Type
- Monoclonal Antibody
- Clone
- RMT4-54
- Isotype
- IgG2a
- Specificity
- TIM-4
Antibody Quality and Performance Guarantee
| Rat anti Mouse TIM-4 antibody, clone RMT4-54 recognizes mouse Tim-4 (T-cell immunoglobulin mucin 4), a transmembrane glycoprotein and member of the immunoglobulin superfamily, exclusively expressed by antigen-presenting cells, which acts as the natural ligand of Tim-1, involved in T-cell expansion and cytokine production. Tim-4 is necessary for the phagocytosis of apoptotic cells by peritoneal macrophages (Nishi et al. 2014) and interaction between Tim-1 on Th2 cells and Tim-4 on DCs, enhances Th2 cell function. The TIM-4 gene locus is associated with predisposition to asthma in both mice and humans and may be considered as a candidate disease gene for asthma. Rat anti Mouse TIM-4 antibody, clone RMT4-54 inhibits the efficient in vitro phagocytosis of apoptotic cells by resident peritoneal Mac1+ cells), but not inflammatory peritoneal exudate Mac1+ cells (Nakayama et al. 2009). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Tim-4-Ig, consisting of amino acids 1-288 of mouse Tim-4, coupled with the Fc portion of mouse IgG2a.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Lymph node cells from immunised SD rats were fused with cells of the P3U1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunofluorescence | |
||
| Immunohistology - Paraffin | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Mouse spleen
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
Source Reference
-
Nakayama, M. et al. (2009) Tim-3 mediates phagocytosis of apoptotic cells and cross-presentation.
Blood. 113 (16): 3821-30.
Further Reading
-
Freeman, G.J. et al. (2010) TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.
Immunol Rev. 235 (1): 172-89.
- Synonyms
- T Cell Immunoglobulin Mucin 4
- UniProt
- Q6U7R4
- Entrez Gene
- Timd4
- GO Terms
- GO:0016021 integral to membrane
MCA5791GA
0811
157508
163606
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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