TIM-1 antibody | RMT1-10

Rat anti Mouse TIM-1

Product Type
Monoclonal Antibody
Clone
RMT1-10
Isotype
IgG2a
Specificity
TIM-1

Product Code Applications Pack Size List Price Your Price Qty
MCA5788GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IF 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse TIM-1 antibody, clone RMT1-10 recognizes mouse Tim-1 (T-cell immunoglobulin mucin 1), a cell surface glycoprotein first identified as the hepatitis A virus cellular receptor (HAVCR). TIM-1 is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b).

Tim-1 is highly expressed by activated CD4+ T cells, acting as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity.

In humans, polymorphic forms of Tim-1 are associated with increased susceptibility to asthma, eczema and rheumatoid arthritis (Rodriguez-Manzanet et al. 2009).

Rat anti Mouse TIM-1 antibody, clone RMT1-10 promotes Th2 responses, and inhibits antigen-specific T cell proliferation, in contrast to the agonistic function of many Tim-1 antibodies (Xiao et al. 2007). Rat anti Mouse TIM-1 antibody, clone RMT1-10 reduces the severity of experimental autoimmune encephalomyelitis and delays disease onset in mice (Xiao et al. 2007).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Full-length mouse Tim-1-Ig, containing both the IgV and mucin domains of Tim-1.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/25 1/200
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rat IgG2a Negative Control

References for TIM-1 antibody

  1. Xiao, S. et al. (2007) Differential engagement of Tim-1 during activation can positively or negatively costimulate T cell expansion and effector function.
    J Exp Med. 204 (7): 1691-702.
  2. Yuan, X. et al. (2009) Targeting Tim-1 to overcome resistance to transplantation tolerance mediated by CD8 T17 cells.
    Proc Natl Acad Sci U S A. 106 (26): 10734-9.
  3. Ueno, T. et al. (2008) The emerging role of T cell Ig mucin 1 in alloimmune responses in an experimental mouse transplant model.
    J Clin Invest. 118: 742-51.
  4. Fukushima, A. et al. (2007) Antibodies to T-cell Ig and mucin domain-containing proteins (Tim)-1 and -3 suppress the induction and progression of murine allergic conjunctivitis.
    Biochem Biophys Res Commun. 353: 211-6.
  5. Rong, S. et al. (2011) The TIM-1:TIM-4 pathway enhances renal ischemia-reperfusion injury.
    J Am Soc Nephrol. 22: 484-95.
  6. Arai, S. et al. (2016) Apoptosis inhibitor of macrophage protein enhances intraluminal debris clearance and ameliorates acute kidney injury in mice.
    Nat Med. 22 (2): 183-93.

Further Reading

  1. Freeman, G.J. et al. (2010) TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.
    Immunol Rev. 235 (1): 172-89.

Flow Cytometry

Functional Assays

Synonyms
CD365
T Cell Immunoglobulin Mucin 1
UniProt
Q5QNS5
Entrez Gene
Havcr1
GO Terms
GO:0016021 integral to membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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