TIM-1 antibody | RMT1-10
Filter by Application:F Reset
|Rat anti Mouse TIM-1 antibody, clone RMT1-10 recognizes mouse Tim-1 (T-cell immunoglobulin mucin 1), a cell surface glycoprotein first identified as the hepatitis A virus cellular receptor (HAVCR). TIM-1 is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b).
Tim-1 is highly expressed by activated CD4+ T cells, acting as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity.
In humans, polymorphic forms of Tim-1 are associated with increased susceptibility to asthma, eczema and rheumatoid arthritis (Rodriguez-Manzanet et al. 2009).
Rat anti Mouse TIM-1 antibody, clone RMT1-10 promotes Th2 responses, and inhibits antigen-specific T cell proliferation, in contrast to the agonistic function of many Tim-1 antibodies (Xiao et al. 2007). Rat anti Mouse TIM-1 antibody, clone RMT1-10 reduces the severity of experimental autoimmune encephalomyelitis and delays disease onset in mice (Xiao et al. 2007).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Full-length mouse Tim-1-Ig, containing both the IgV and mucin domains of Tim-1.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for TIM-1 antibody
Xiao, S. et al. (2007) Differential engagement of Tim-1 during activation can positively or negatively costimulate T cell expansion and effector function.
J Exp Med. 204 (7): 1691-702.
Yuan, X. et al. (2009) Targeting Tim-1 to overcome resistance to transplantation tolerance mediated by CD8 T17 cells.
Proc Natl Acad Sci U S A. 106 (26): 10734-9.
Ueno, T. et al. (2008) The emerging role of T cell Ig mucin 1 in alloimmune responses in an experimental mouse transplant model.
J Clin Invest. 118: 742-51.
Fukushima, A. et al. (2007) Antibodies to T-cell Ig and mucin domain-containing proteins (Tim)-1 and -3 suppress the induction and progression of murine allergic conjunctivitis.
Biochem Biophys Res Commun. 353: 211-6.
Rong, S. et al. (2011) The TIM-1:TIM-4 pathway enhances renal ischemia-reperfusion injury.
J Am Soc Nephrol. 22: 484-95.
Arai, S. et al. (2016) Apoptosis inhibitor of macrophage protein enhances intraluminal debris clearance and ameliorates acute kidney injury in mice.
Nat Med. 22 (2): 183-93.
Freeman, G.J. et al. (2010) TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.
Immunol Rev. 235 (1): 172-89.
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.Yes, sign me up