PLVAP antibody | MECA-32
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Rat anti Mouse PLVAP
- Product Type
- Monoclonal Antibody
- Clone
- MECA-32
- Isotype
- IgG2a
- Specificity
- PLVAP
Antibody Quality and Performance Guarantee
| Rat anti Mouse PLVAP antibody, clone MECA-32 recognizes murine plasmalemma vesicle-associated protein (PLVAP), a ~60 kDa protein also known as MECA-32 and PV-1. PLVAP is a transmembrane homodimer associated with endothelial cell caveolae and fenestrae. In adult and embryonic mice, PLVAP is primarily expressed on endothelial tissues but shows restricted distribution in skeletal, cardiac and brain tissues. In embryonic mice, PLVAP expression, on the vasculature associated with blood brain barrier has been reported to be developmentally regulated. In humans, PLVAP is selectively up-regulated in a variety of high-grade brain tumors, including glioblastoma and metastatic carcinoma, as well as other cerebral disorders associated with blood-brain barrier disruption, such as acute ischemia. PLVAP represents a novel marker of brain tumour angiogenesis and integrity of the blood-brain barrier and is a potential therapeutic target. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from an immunized rat were fused with cells of the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
1/25 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for PLVAP antibody
-
Hallmann, R. et al. (1995) Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
Dev Dyn. 202 (4): 325-32. -
Orford M et al. (2009) Generation of an ABCG2(GFPn-puro) transgenic line--a tool to study ABCG2 expression in mice.
Biochem Biophys Res Commun. 384 (2): 199-203. -
Daneman, R. et al. (2010) Pericytes are required for blood-brain barrier integrity during embryogenesis.
Nature. 468 (7323): 562-6. -
Okizaki, S. et al. (2016) Vascular Endothelial Growth Factor Receptor Type 1 Signaling Prevents Delayed Wound Healing in Diabetes by Attenuating the Production of IL-1β by Recruited Macrophages.
Am J Pathol. 186 (6): 1481-98.
Further Reading
-
Carson-Walter, E.B. et al. (2005) Plasmalemmal vesicle associated protein-1 is a novel marker implicated in brain tumor angiogenesis.
Clin Cancer Res. 11 (21): 7643-50.
- Synonyms
- MECA-32
- Plasmalemma Vesicle Associated Protein
- RRID
- AB_931736
- UniProt
- Q91VC4
- Entrez Gene
- Plvap
- GO Terms
- GO:0016021 integral to membrane
- GO:0005901 caveola
- GO:0048471 perinuclear region of cytoplasm
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