PLVAP antibody | MECA-32

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Rat anti Mouse PLVAP:Biotin

Rat anti Mouse PLVAP

Product Type
Monoclonal Antibody
Clone
MECA-32
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2539B C 0.1 mg
MCA2539T C 25 µg
MCA2539GA C 0.1 mg
MCA2539 C 0.25 mg
Rat anti Mouse PLVAP antibody, clone MECA-32 recognizes murine plasmalemma vesicle-associated protein (PLVAP), a ~60 kDa protein also known as MECA-32 and PV-1. PLVAP is a transmembrane homodimer associated with endothelial cell caveolae and fenestrae. In adult and embryonic mice, PLVAP is primarily expressed on endothelial tissues but shows restricted distribution in skeletal, cardiac and brain tissues. In embryonic mice, PLVAP expression, on the vasculature associated with blood brain barrier has been reported to be developmentally regulated.

In humans, PLVAP is selectively up-regulated in a variety of high-grade brain tumors, including glioblastoma and metastatic carcinoma, as well as other cerebral disorders associated with blood-brain barrier disruption, such as acute ischemia. PLVAP represents a novel marker of brain tumour angiogenesis and integrity of the blood-brain barrier and is a potential therapeutic target.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from an immunized rat were fused with cells of the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q91VC4 Related reagents
Entrez Gene
Plvap Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005901 caveola
GO:0048471 perinuclear region of cytoplasm
Regulatory
For research purposes only

Applications of PLVAP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen Neat 1/10
Immunohistology - Frozen 1/25 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Product Specific References

References for PLVAP antibody

  1. Hallmann, R. et al. (1995) Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Dev Dyn. 202 (4): 325-32.
  2. Daneman, R. et al. (2010) Pericytes are required for blood-brain barrier integrity during embryogenesis.
    Nature. 468 (7323): 562-6.
  3. Okizaki SI et al. (2016) Vascular Endothelial Growth Factor Receptor Type 1 Signaling Prevents Delayed Wound Healing in Diabetes by Attenuating the Production of IL-1β by Recruited Macrophages.
    Am J Pathol. Apr 13. pii: S0002-9440(16)30030-X. [Epub ahead of print]
  4. Orford M et al. (2009) Generation of an ABCG2(GFPn-puro) transgenic line--a tool to study ABCG2 expression in mice.
    Biochem Biophys Res Commun. 384 (2): 199-203.

Further Reading

  1. Carson-Walter, E.B. et al. (2005) Plasmalemmal vesicle associated protein-1 is a novel marker implicated in brain tumor angiogenesis.
    Clin Cancer Res. 11 (21): 7643-50.