PLVAP antibody | MECA-32

Rat anti Mouse PLVAP

Product Type
Monoclonal Antibody
Clone
MECA-32
Isotype
IgG2a
Specificity
PLVAP

Product Code Applications Pack Size List Price Your Price Qty
MCA2539GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C 0.1 mg loader
List Price Your Price
loader
MCA2539T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C 25 µg loader
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loader
MCA2539
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse PLVAP antibody, clone MECA-32 recognizes murine plasmalemma vesicle-associated protein (PLVAP), a ~60 kDa protein also known as MECA-32 and PV-1. PLVAP is a transmembrane homodimer associated with endothelial cell caveolae and fenestrae. In adult and embryonic mice, PLVAP is primarily expressed on endothelial tissues but shows restricted distribution in skeletal, cardiac and brain tissues. In embryonic mice, PLVAP expression, on the vasculature associated with blood brain barrier has been reported to be developmentally regulated.

In humans, PLVAP is selectively up-regulated in a variety of high-grade brain tumors, including glioblastoma and metastatic carcinoma, as well as other cerebral disorders associated with blood-brain barrier disruption, such as acute ischemia. PLVAP represents a novel marker of brain tumour angiogenesis and integrity of the blood-brain barrier and is a potential therapeutic target.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from an immunized rat were fused with cells of the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/25 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
List Price Your Price
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for PLVAP antibody

  1. Hallmann, R. et al. (1995) Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Dev Dyn. 202 (4): 325-32.
  2. Daneman, R. et al. (2010) Pericytes are required for blood-brain barrier integrity during embryogenesis.
    Nature. 468 (7323): 562-6.
  3. Okizaki SI et al. (2016) Vascular Endothelial Growth Factor Receptor Type 1 Signaling Prevents Delayed Wound Healing in Diabetes by Attenuating the Production of IL-1β by Recruited Macrophages.
    Am J Pathol. Apr 13. pii: S0002-9440(16)30030-X. [Epub ahead of print]
  4. Orford M et al. (2009) Generation of an ABCG2(GFPn-puro) transgenic line--a tool to study ABCG2 expression in mice.
    Biochem Biophys Res Commun. 384 (2): 199-203.

Further Reading

  1. Carson-Walter, E.B. et al. (2005) Plasmalemmal vesicle associated protein-1 is a novel marker implicated in brain tumor angiogenesis.
    Clin Cancer Res. 11 (21): 7643-50.

Synonyms
MECA-32
Plasmalemma Vesicle Associated Protein
RRID
AB_931736
UniProt
Q91VC4
Entrez Gene
Plvap
GO Terms
GO:0016021 integral to membrane
GO:0005901 caveola
GO:0048471 perinuclear region of cytoplasm
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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