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P2X7 antibody | Hano43

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Rat anti Mouse P2X7

Product Type
Monoclonal Antibody
Clone
Hano43
Isotype
IgG2b
Specificity
P2X7

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Product Code Applications Pack Size List Price Your Price Qty
MCA4713
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 0.25 mg loader
List Price Your Price
loader
MCA4713GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 0.1 mg loader
List Price Your Price
loader

Rat anti Mouse P2X7 antibody, clone Hano43 recognizes the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
A P2X7-expression construct and a final boost with P2X7-transfected HEK cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed) STAR71D550 F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for P2X7 antibody

  1. Adriouch, S. et al. (2005) Probing the expression and function of the P2X7 purinoceptor with antibodies raised by genetic immunization.
    Cell Immunol. 236: 72-7.
  2. Adriouch, S. et al. (2008) ADP-ribosylation at R125 gates the P2X7 ion channel by presenting a covalent ligand to its nucleotide binding site.
    FASEB J. 2008 Mar;22(3):861-9.
  3. Aswad, F. and Dennert, G. (2006) P2X7 receptor expression levels determine lethal effects of a purine based danger signal in T lymphocytes.
    Cell Immunol. 243: 58-65.
  4. Adriouch, S. et al. (2009) Characterisation of the R276A gain-of-function mutation in the ectodomain of murine P2X7.
    Purinergic Signal. 5: 151-61.
  5. Kurashima, Y. et al. (2012) Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors.
    Nat Commun. 3: 1034.
  6. Hu, S.J. et al. (2015) Upregulation of P2RX7 in Cx3cr1-Deficient Mononuclear Phagocytes Leads to Increased Interleukin-1β Secretion and Photoreceptor Neurodegeneration.
    J Neurosci. 35 (18): 6987-96.
  7. Barabási, B. et al. (2016) Effect of axotomy and 17β-estradiol on P2X7 receptor expression pattern in the hypoglossal nucleus of ovariectomized mice.
    Neuroscience. 319: 107-15.
  8. Liu, Q. & Kim, C.H. (2019) Control of Tissue-Resident Invariant NKT Cells by Vitamin A Metabolites and P2X7-Mediated Cell Death.
    J Immunol. 203 (5): 1189-97.
    9. View The Latest Product References

Further Reading

  1. Ferrari, D. et al. (2006) The P2X7 receptor: a key player in IL-1 processing and release.
    J Immunol. 176: 3877-83.
  2. Schwarz, N. et al (2009) Activation of the P2X7 ion channel by soluble and covalently bound ligands.
    Purinergic Signal. 5: 139-49.

Flow Cytometry

Synonyms
P2RX7
RRID
AB_10545124
UniProt
Q9Z1M0
Entrez Gene
P2rx7
GO Terms
GO:0000187 activation of MAPK activity
GO:0000902 cell morphogenesis
GO:0001530 lipopolysaccharide binding
GO:0001845 phagolysosome assembly
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0004931 extracellular ATP-gated cation channel activity
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0005911 cell-cell junction
GO:0006509 membrane protein ectodomain proteolysis
GO:0006649 phospholipid transfer to membrane
GO:0006800 oxygen and reactive oxygen species metabolic process
GO:0006884 cell volume homeostasis
GO:0006900 membrane budding
GO:0009612 response to mechanical stimulus
GO:0006954 inflammatory response
GO:0007005 mitochondrion organization
GO:0007009 plasma membrane organization
GO:0019835 cytolysis
GO:0050830 defense response to Gram-positive bacterium
GO:0042493 response to drug
GO:0009897 external side of plasma membrane
GO:0010043 response to zinc ion
GO:0014049 positive regulation of glutamate secretion
GO:0014054 positive regulation of gamma-aminobutyric acid secretion
GO:0014070 response to organic cyclic compound
GO:0046931 pore complex assembly
GO:0016079 synaptic vesicle exocytosis
GO:0016485 protein processing
GO:0019233 sensory perception of pain
GO:0019717 synaptosome
GO:0030501 positive regulation of bone mineralization
GO:0031594 neuromuscular junction
GO:0031668 cellular response to extracellular stimulus
GO:0032060 bleb assembly
GO:0032308 positive regulation of prostaglandin secretion
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0032963 collagen metabolic process
GO:0033198 response to ATP
GO:0034405 response to fluid shear stress
GO:0042098 T cell proliferation
GO:0043025 neuronal cell body
GO:0043029 T cell homeostasis
GO:0043065 positive regulation of apoptosis
GO:0043132 NAD transport
GO:0043409 negative regulation of MAPKKK cascade
GO:0043410 positive regulation of MAPKKK cascade
GO:0044254 multicellular organismal protein catabolic process
GO:0045332 phospholipid translocation
GO:0045779 negative regulation of bone resorption
GO:0046513 ceramide biosynthetic process
GO:0048705 skeletal system morphogenesis
GO:0048873 homeostasis of number of cells within a tissue
GO:0050717 positive regulation of interleukin-1 alpha secretion
GO:0050718 positive regulation of interleukin-1 beta secretion
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051259 protein oligomerization
GO:0051592 response to calcium ion
GO:0051602 response to electrical stimulus
GO:0051789 response to protein stimulus
GO:0051901 positive regulation of mitochondrial depolarization

MCA4713

159478 1801

MCA4713GA

149134 155563 164684

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