P2X7 antibody | Hano43
|Rat anti Mouse P2X7 antibody, clone Hano43 recognizes the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- A P2X7-expression construct and a final boost with P2X7-transfected HEK cells.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised rats were fused with cells of the Sp2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for P2X7 antibody
Adriouch, S. et al. (2005) Probing the expression and function of the P2X7 purinoceptor with antibodies raised by genetic immunization.
Cell Immunol. 236: 72-7.
Adriouch, S. et al. (2008) ADP-ribosylation at R125 gates the P2X7 ion channel by presenting a covalent ligand to its nucleotide binding site.
FASEB J. 2008 Mar;22(3):861-9.
Schwarz, N. et al (2009) Activation of the P2X7 ion channel by soluble and covalently bound ligands.
Purinergic Signal. 5: 139-49.
Aswad, F. and Dennert, G. (2006) P2X7 receptor expression levels determine lethal effects of a purine based danger signal in T lymphocytes.
Cell Immunol. 243: 58-65.
Adriouch, S. et al. (2009) Characterisation of the R276A gain-of-function mutation in the ectodomain of murine P2X7.
Purinergic Signal. 5: 151-61.
Danquah, W. et al. (2010) Preventing gating of the P2X7 ion channel in vitro and in vivo with monoclonal antibodies and recombinant single domain Nanobodies
Purines 2010: Adenine Nucleosides and Nucleotides in Biomedicine Meeting: Abstract P2-24
Kurashima, Y. et al. (2012) Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors.
Nat Commun. 3: 1034.
Hu, S.J. et al. (2015) Upregulation of P2RX7 in Cx3cr1-Deficient Mononuclear Phagocytes Leads to Increased Interleukin-1β Secretion and Photoreceptor Neurodegeneration.
J Neurosci. 35 (18): 6987-96.
View The Latest Product References
Barabási, B. et al. (2016) Effect of axotomy and 17β-estradiol on P2X7 receptor expression pattern in the hypoglossal nucleus of ovariectomized mice.
Neuroscience. 319: 107-15.
Liu, Q. & Kim, C.H. (2019) Control of Tissue-Resident Invariant NKT Cells by Vitamin A Metabolites and P2X7-Mediated Cell Death.
J Immunol. 203 (5): 1189-97.
Ferrari, D. et al. (2006) The P2X7 receptor: a key player in IL-1 processing and release.
J Immunol. 176: 3877-83.
- Entrez Gene
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0000902 cell morphogenesis
- GO:0001530 lipopolysaccharide binding
- GO:0001845 phagolysosome assembly
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0004931 extracellular ATP-gated cation channel activity
- GO:0005887 integral to plasma membrane
- GO:0005524 ATP binding
- GO:0005911 cell-cell junction
- View More GO Terms
- GO:0006509 membrane protein ectodomain proteolysis
- GO:0006649 phospholipid transfer to membrane
- GO:0006800 oxygen and reactive oxygen species metabolic process
- GO:0006884 cell volume homeostasis
- GO:0006900 membrane budding
- GO:0009612 response to mechanical stimulus
- GO:0006954 inflammatory response
- GO:0007005 mitochondrion organization
- GO:0007009 plasma membrane organization
- GO:0019835 cytolysis
- GO:0050830 defense response to Gram-positive bacterium
- GO:0042493 response to drug
- GO:0009897 external side of plasma membrane
- GO:0010043 response to zinc ion
- GO:0014049 positive regulation of glutamate secretion
- GO:0014054 positive regulation of gamma-aminobutyric acid secretion
- GO:0014070 response to organic cyclic compound
- GO:0046931 pore complex assembly
- GO:0016079 synaptic vesicle exocytosis
- GO:0016485 protein processing
- GO:0019233 sensory perception of pain
- GO:0019717 synaptosome
- GO:0030501 positive regulation of bone mineralization
- GO:0031594 neuromuscular junction
- GO:0031668 cellular response to extracellular stimulus
- GO:0032060 bleb assembly
- GO:0032308 positive regulation of prostaglandin secretion
- GO:0032496 response to lipopolysaccharide
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032963 collagen metabolic process
- GO:0033198 response to ATP
- GO:0034405 response to fluid shear stress
- GO:0042098 T cell proliferation
- GO:0043025 neuronal cell body
- GO:0043029 T cell homeostasis
- GO:0043065 positive regulation of apoptosis
- GO:0043132 NAD transport
- GO:0043409 negative regulation of MAPKKK cascade
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0044254 multicellular organismal protein catabolic process
- GO:0045332 phospholipid translocation
- GO:0045779 negative regulation of bone resorption
- GO:0046513 ceramide biosynthetic process
- GO:0048705 skeletal system morphogenesis
- GO:0048873 homeostasis of number of cells within a tissue
- GO:0050717 positive regulation of interleukin-1 alpha secretion
- GO:0050718 positive regulation of interleukin-1 beta secretion
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051259 protein oligomerization
- GO:0051592 response to calcium ion
- GO:0051602 response to electrical stimulus
- GO:0051789 response to protein stimulus
- GO:0051901 positive regulation of mitochondrial depolarization
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