P2X7 antibody | Hano43

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Rat anti Mouse P2X7:Alexa Fluor® 488

Rat anti Mouse P2X7:Alexa Fluor® 647

Rat anti Mouse P2X7:FITC

Rat anti Mouse P2X7

Product Type
Monoclonal Antibody
Clone
Hano43
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4713A488 F 100 Tests/1ml
MCA4713A647 F 100 Tests/1ml
MCA4713F F 0.1 mg
MCA4713GA F IF 0.1 mg
MCA4713 F IF 0.25 mg
Rat anti Mouse P2X7 antibody, clone Hano43 recognizes the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
A P2X7-expression construct and a final boost with P2X7-transfected HEK cells.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9Z1M0 Related reagents
Entrez Gene
P2rx7 Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0000902 cell morphogenesis
GO:0001530 lipopolysaccharide binding
GO:0001845 phagolysosome assembly
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0004931 extracellular ATP-gated cation channel activity
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0005911 cell-cell junction
GO:0006509 membrane protein ectodomain proteolysis
GO:0006649 phospholipid transfer to membrane
GO:0006800 oxygen and reactive oxygen species metabolic process
GO:0006884 cell volume homeostasis
GO:0006900 membrane budding
GO:0009612 response to mechanical stimulus
GO:0006954 inflammatory response
GO:0007005 mitochondrion organization
GO:0007009 plasma membrane organization
GO:0019835 cytolysis
GO:0050830 defense response to Gram-positive bacterium
GO:0042493 response to drug
GO:0009897 external side of plasma membrane
GO:0010043 response to zinc ion
GO:0014049 positive regulation of glutamate secretion
GO:0014054 positive regulation of gamma-aminobutyric acid secretion
GO:0014070 response to organic cyclic compound
GO:0046931 pore complex assembly
GO:0016079 synaptic vesicle exocytosis
GO:0016485 protein processing
GO:0019233 sensory perception of pain
GO:0019717 synaptosome
GO:0030501 positive regulation of bone mineralization
GO:0031594 neuromuscular junction
GO:0031668 cellular response to extracellular stimulus
GO:0032060 bleb assembly
GO:0032308 positive regulation of prostaglandin secretion
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0032963 collagen metabolic process
GO:0033198 response to ATP
GO:0034405 response to fluid shear stress
GO:0042098 T cell proliferation
GO:0043025 neuronal cell body
GO:0043029 T cell homeostasis
GO:0043065 positive regulation of apoptosis
GO:0043132 NAD transport
GO:0043409 negative regulation of MAPKKK cascade
GO:0043410 positive regulation of MAPKKK cascade
GO:0044254 multicellular organismal protein catabolic process
GO:0045332 phospholipid translocation
GO:0045779 negative regulation of bone resorption
GO:0046513 ceramide biosynthetic process
GO:0048705 skeletal system morphogenesis
GO:0048873 homeostasis of number of cells within a tissue
GO:0050717 positive regulation of interleukin-1 alpha secretion
GO:0050718 positive regulation of interleukin-1 beta secretion
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051259 protein oligomerization
GO:0051592 response to calcium ion
GO:0051602 response to electrical stimulus
GO:0051789 response to protein stimulus
GO:0051901 positive regulation of mitochondrial depolarization
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of P2X7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry
Flow Cytometry
Flow Cytometry 1/25 1/200
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Product Specific References

References for P2X7 antibody

  1. Adriouch, S. et al. (2005) Probing the expression and function of the P2X7 purinoceptor with antibodies raised by genetic immunization.
    Cell Immunol. 236: 72-7.
  2. Adriouch, S. et al. (2008) ADP-ribosylation at R125 gates the P2X7 ion channel by presenting a covalent ligand to its nucleotide binding site.
    FASEB J. 2008 Mar;22(3):861-9.
  3. Schwarz, N. et al (2009) Activation of the P2X7 ion channel by soluble and covalently bound ligands.
    Purinergic Signal. 5: 139-49.
  4. Aswad, F. and Dennert, G. (2006) P2X7 receptor expression levels determine lethal effects of a purine based danger signal in T lymphocytes.
    Cell Immunol. 243: 58-65.
  5. Adriouch, S. et al. (2009) Characterisation of the R276A gain-of-function mutation in the ectodomain of murine P2X7.
    Purinergic Signal. 5: 151-61.
  6. Danquah, W. et al. (2010) Preventing gating of the P2X7 ion channel in vitro and in vivo with monoclonal antibodies and recombinant single domain Nanobodies
    Purines 2010: Adenine Nucleosides and Nucleotides in Biomedicine Meeting: Abstract P2-24
  7. Kurashima, Y. et al. (2012) Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors.
    Nat Commun. 3: 1034.
  8. Hu, S.J. et al. (2015) Upregulation of P2RX7 in Cx3cr1-Deficient Mononuclear Phagocytes Leads to Increased Interleukin-1╬▓ Secretion and Photoreceptor Neurodegeneration.
    J Neurosci. 35 (18): 6987-96.
  9. Barabási, B. et al. (2016) Effect of axotomy and 17β-estradiol on P2X7 receptor expression pattern in the hypoglossal nucleus of ovariectomized mice.
    Neuroscience. 319: 107-15.

Further Reading

  1. Ferrari, D. et al. (2006) The P2X7 receptor: a key player in IL-1 processing and release.
    J Immunol. 176: 3877-83.