Notch 2 antibody | HMN2-35

100% Secure


Hamster anti Mouse Notch 2

Product Type
Monoclonal Antibody
Clone
HMN2-35
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5702GA F 0.1 mg
MCA5702 F 0.25 mg
Hamster anti Mouse Notch 2 antibody, clone HMN2-35 recognizes Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands.

The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression.

Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.

Signaling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukemia (B-CLL). Studies have also shown a correlation between a decrease in Notch 2 expression and an increase in grade of human breast cancer.

Hamster anti Mouse Notch 2 antibody, clone HMN2-35 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Mouse Notch 2-Fc fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O35516 Related reagents
Q9QW30 Related reagents
Entrez Gene
Notch2 Related reagents
Notch2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0016049 cell growth
GO:0001709 cell fate determination
GO:0002011 morphogenesis of an epithelial sheet
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005634 nucleus
GO:0006350 transcription
GO:0007050 cell cycle arrest
GO:0007219 Notch signaling pathway
GO:0007368 determination of left/right symmetry
GO:0009887 organ morphogenesis
GO:0008285 negative regulation of cell proliferation
GO:0009986 cell surface
GO:0030326 embryonic limb morphogenesis
GO:0043065 positive regulation of apoptosis
GO:0045449 regulation of transcription
GO:0046579 positive regulation of Ras protein signal transduction
GO:0050793 regulation of developmental process
GO:0042246 tissue regeneration
Regulatory
For research purposes only

Applications of Notch 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F

Product Specific References

References for Notch 2 antibody

  1. Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.
    Int Immunol. 20 (6): 763-73.
  2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.
    Int Immunol. 21 (3): 295-301.
  3. Gibb, D.R. et al. (2010) ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo.
    J Exp Med. 207 (3): 623-35.
  4. Sakata-Yanagimoto, M. et al. (2011) Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine.
    Blood. 117 (1): 128-34.

Further Reading

  1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
    Nat Rev Mol Cell Biol. 7 (9): 678-89.
  2. Iso, T. et al. (2003) Notch signaling in vascular development.
    Arterioscler Thromb Vasc Biol. 23 (4): 543-53.
  3. Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways.
    Immunity. 29 (5): 691-703.
  4. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.
    Immunol Rev. 182: 215-27.