MHC Class II H-2I-Ak/s/R antibody | ER-TR2
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Rat anti Mouse MHC Class II H-2I-Ak/s/R antibody, clone ER-TR2 recognizes a polymorphic determinant of the mouse H-2I-A MHC class II antigen, reacting with haplotypes k, s and r. Mouse strains with the haplotypes b, d, q and f are not recognized by this antibody.
Mouse MHC class II antigens are expressed by dendritic cells, B-cells and macrophages. In thymus tissues, clone ER-TR2 stains cortical and medullary stromal cells.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region.
Mouse MHC class II antigens are expressed by dendritic cells, B-cells and macrophages. In thymus tissues, clone ER-TR2 stains cortical and medullary stromal cells.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Isolated C3H thymic stromal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.56 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class II H-2I-Ak/s/R antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/100 | 1/200 | |
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Product Specific References
Source Reference
-
Van Vliet, E. et al. (1984) Monoclonal antibodies to stromal cell types of the mouse thymus.
Eur J Immunol. 14 (6): 524-9.
References for MHC Class II H-2I-Ak/s/R antibody
-
Huiskamp, R. et al. (1985) Repopulation of the mouse thymus after sublethal fission neutron irradiation. II. Sequential changes in the thymic microenvironment.
J Immunol. 134 (4): 2170-8.
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