MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody | ER-MP42

Rat anti Mouse MHC Class I H-2Dd H-2Kv H-2k/Q/S

Product Type
Monoclonal Antibody
Clone
ER-MP42
Isotype
IgG2a
Specificity
MHC Class I H-2Dd H-2Kv H-2k/Q/S

Product Code Applications Pack Size List Price Your Price Qty
MCA2397
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody, clone ER-MP42 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells.

Clone ER-MP42 specifically recognizes mouse strains with the haplotypes H-2Kv, H-2Dd and H-2k, q, s. Mouse strains with the haplotypes H-2p, r, w7, w22 show weak reactivity with this antibody.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Balb/c macrophage precursor cell hybrids.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised Lewis rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Rat IgG2a Negative Control

References for MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody

  1. Medana, I. et al. (2001) Transection of major histocompatibility complex class I-induced neurites by cytotoxic T lymphocytes.
    Am J Pathol. 159 (3): 809-15.

RRID
AB_877510
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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