MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody | ER-MP42
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Rat anti Mouse MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody, clone ER-MP42 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells.
Clone ER-MP42 specifically recognizes mouse strains with the haplotypes H-2Kv, H-2Dd and H-2k, q, s. Mouse strains with the haplotypes H-2p, r, w7, w22 show weak reactivity with this antibody.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Clone ER-MP42 specifically recognizes mouse strains with the haplotypes H-2Kv, H-2Dd and H-2k, q, s. Mouse strains with the haplotypes H-2p, r, w7, w22 show weak reactivity with this antibody.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Balb/c macrophage precursor cell hybrids.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised Lewis rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/100 | 1/200 | |
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Product Specific References
References for MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody
-
Medana, I. et al. (2001) Transection of major histocompatibility complex class I-induced neurites by cytotoxic T lymphocytes.
Am J Pathol. 159 (3): 809-15.
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