MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody | ER-MP42

Rat anti Mouse MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody, clone ER-MP42 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells.

Clone ER-MP42 specifically recognizes mouse strains with the haplotypes H-2Kv, H-2Dd and H-2k, q, s. Mouse strains with the haplotypes H-2p, r, w7, w22 show weak reactivity with this antibody.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.

Product Details

Target Species

Mouse

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Balb/c macrophage precursor cell hybrids.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Cells from immunised Lewis rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.

Storage Information

Storage

Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

More Information

Regulatory

For research purposes only

Applications of MHC Class I H-2Dd H-2Kv H-2k/Q/S antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/100	1/200
Immunohistology - Frozen

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

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