MARCO antibody | ED31
Rat anti Mouse MARCO antibody, clone ED31 (MCA1849) used for the evaluation of MARCO expression of spleen cells by immunofluorescence.
Image caption:
Marginal zone macrophages (MZM) efficiently traffic antigen from the circulation to the follicle. (A) Fluorescently labeled model antigen (green, Dextran-FITC) is trapped in the spleen by MZM (red, MARCO) in spleens from animals recovered 2 days from middle cerebral artery occlusion (MCAO) or sham surgery or naïve controls 1 h after iv injection. By 24-h post iv injection, antigen has been trafficked into the follicle of the white pulp (WP). Naïve animals were injected with vehicle (PBS) as a control.
From: McCulloch L, Alfieri A, McColl BW.
Experimental Stroke Differentially Affects Discrete Subpopulations of Splenic Macrophages.
Front Immunol. 2018 May 22;9:1108.
doi: 10.3389/fimmu.2018.01108.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse MARCO antibody, clone ED31 (MCA1849) used for the evaluation of MARCO expression of spleen cells by immunofluorescence.
Image caption:
Increased density of splenic macrophage subsets is not due to increased recruitment from bone marrow.(F) Increased immunofluorescent labeling of proliferation markers (white, KI-67) associated with MARCO+ marginal zone macrophages (green, MARCO) or red pulp macrophages (red, F4/80) in the spleens of mice recovered 2 days after MCAO in comparison to sham-operated animals (sham n = 4, MCAO n = 4). Scale bar 50 µm. Data show mean ± SD; unpaired t-test.
From: McCulloch L, Alfieri A, McColl BW.
Experimental Stroke Differentially Affects Discrete Subpopulations of Splenic Macrophages.
Front Immunol. 2018 May 22;9:1108.
doi: 10.3389/fimmu.2018.01108.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse MARCO antibody, clone ED31 (MCA1849) used for the evaluation of MARCO expression of spleen cells by immunofluorescence.
Image caption:
Experimental stroke differentially affects marginal zone (MZ) and white pulp (WP) macrophage populations. (A) Immunofluorescent labeling of spleens from mice recovered 1–7 days after middle cerebral artery occlusion (MCAO) for marginal zone macrophages (MZM) (green, MARCO), marginal zone metallophillic macrophages (red, MOMA-1), tingible body macrophages (red, MOMA-2 within WP), classical dendritic cells (blue, CD11c), and DEC205+ DC (red, DEC205).
From: McCulloch L, Alfieri A, McColl BW.
Experimental Stroke Differentially Affects Discrete Subpopulations of Splenic Macrophages.
Front Immunol. 2018 May 22;9:1108.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse MARCO antibody, clone ED31 (MCA1849) used for the identification of marginal zone macrophages in murine spleen sections by immunofluorescence.
Image caption:
Neutrophils correlate with the MZ B cell region in the spleen. Immunofluorescence staining of adult spleen sections. (a) Anti-MARCO stains the MZ macrophages, correlated with the area of IgM high-expressing MZ B cells. The Ly6G antibody marks the splenic neutrophil in the peri-MZ region, surrounding the white pulp (WP) and proximal to the MZ B cells and macrophages. Pictures are shown in overview of WP and high magnification of the MZ structures. (b) Anti-MOMA-1 labels the metallophilic macrophages, which locates at the inner ring of the MZ region and rarely in touch with spleen neutrophils. Scale bars represent 100μm. Data represent two independent experiments with two mice each experiment.
From: Zhao Z, Luo Z, Yang Q, et al.
Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis.
J Immunol Res. 2019, Article ID 9561350, 11 pages.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse MARCO antibody, clone ED31 (MCA1849) used for the evaluation of MARCO expression of spleen cells by immunofluorescence.
Image caption:
Macrophage subpopulation-specific impairments in expression of MHC Class II. Genome-wide transcriptional analysis was performed by microarray of RNA extracted from spleen tissue of mice recovered 1, 2, or 5 days after middle cerebral artery occlusion (MCAO) or sham surgery. The fold-change of macrophage-associated gene expression levels was analyzed in relation to expression measured in spleens from sham-operated animals (A) Analysis of the H2 genes which encode mouse MHC class II show downregulation of expression of all H2 genes 1–5 days after MCAO in comparison to sham-operated animals (sham n = 2, 1 day and 2 days n = 3, 5 days n = 4). (B) MHC Class II (green) immunolabelling along with labeling for marginal zone macrophages (MZM) (red, MARCO; top panel), both tissue resident and monocyte-derived RPM (blue, F4/80 top panel), tingible body macrophages (red, MOMA-2 within white pulp; middle panel), classical dendritic cells (blue, CD11c middle panel), marginal zone metallophillic macrophages (red, MOMA-1; bottom panel) or monocyte-derived RPM only (blue, CD11b; bottom panel) to determine co-localization of MHC Class II on splenic macrophage subsets.
From: McCulloch L, Alfieri A, McColl BW.
Experimental Stroke Differentially Affects Discrete Subpopulations of Splenic Macrophages.
Front Immunol. 2018 May 22;9:1108.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse MARCO antibody, clone ED31 (MCA1849) used for the evaluation of MARCO expression of spleen cells by immunofluorescence.
Image caption:
Macrophage subpopulation-specific increases in markers of lysosome activity after experimental stroke. (C) Immunofluorescent labeling of LAMP2 (green, LAMP2) along with labeling for both tissue resident and monocyte-derived RPM (blue, F4/80; top panel) marginal zone macrophages (magenta, MARCO; top panel), marginal zone metallophillic macrophages (red, MOMA-1; middle panel), monocyte-derived RPM only (blue, CD11b; middle panel), or tingible body macrophages (red, MOMA-2 within the WP; bottom panel) in spleens from naïve mice, or mice recovered 1–7 days after MCAO or sham surgery (naïve n = 4, sham n = 4, 1 day n = 4, 2 days n = 9, 5 days n = 5, 7 days n = 7).
From: McCulloch L, Alfieri A, McColl BW.
Experimental Stroke Differentially Affects Discrete Subpopulations of Splenic Macrophages.
Front Immunol. 2018 May 22;9:1108.
doi: 10.3389/fimmu.2018.01108.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse MARCO
- Product Type
- Monoclonal Antibody
- Clone
- ED31
- Isotype
- IgG1
- Specificity
- MARCO
Antibody Quality and Performance Guarantee
| Rat anti Mouse MARCO antibody, clone ED31 recognizes the murine cell surface antigen designated MARCO (Macrophage receptor with collagenous structure), which is a member of the class A scavenger receptor family. MARCO is expressed by distinct populations of macrophages in the spleen and lymph nodes, but is rapidly induced on macrophages in other tissues (e.g. Liver Kupffer cells) during infection or LPS treatment. Rat anti Mouse MARCO antibody, clone ED31 binds to the C-terminal cysteine rich domain of MARCO, and has been shown to block ligand binding. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- CHO cells expressing mouse MARCO.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised rats were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunofluorescence |  |
||
| Immunohistology - Frozen | |
1/200 | 1/400 |
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for MARCO antibody
-
van der Laan, L.J. et al. (1997) Macrophage scavenger receptor MARCO: in vitro and in vivo regulation and involvement in the anti-bacterial host defense.
Immunol Lett. 57 (1-3): 203-8. -
van der Laan, L.J. et al. (1999) Regulation and functional involvement of macrophage scavenger receptor MARCO in clearance of bacteria in vivo.
J Immunol. 162 (2): 939-47. -
Whitman, S.C. et al. (2002) Macrophage-specific expression of class A scavenger receptors in LDL receptor(-/-) mice decreases atherosclerosis and changes spleen morphology.
J Lipid Res. 43: 1201-8. -
Granucci, F. et al. (2003) The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia.
Blood. 102: 2940-7. -
Karlsson, M.C. et al. (2003) Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone.
J Exp Med. 198: 333-40. -
Grolleau, A. et al. (2003) Inducible expression of macrophage receptor Marco by dendritic cells following phagocytic uptake of dead cells uncovered by oligonucleotide arrays.
J Immunol. 171: 2879-88. -
Taylor, P.R. et al. (2004) Development of a specific system for targeting protein to metallophilic macrophages.
Proc Natl Acad Sci U S A. 101: 1963-8. -
Kang, Y.S. et al. (2004) The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.
Proc Natl Acad Sci U S A. 101: 215-20.
View The Latest Product References
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Alarcón, R. et al. (2005) Expression of scavenger receptors in glial cells. Comparing the adhesion of astrocytes and microglia from neonatal rats to surface-bound beta-amyloid.
J Biol Chem. 280: 30406-15. -
Anthony, R.M. et al. (2008) Identification of a receptor required for the anti-inflammatory activity of IVIG.
Proc Natl Acad Sci U S A. 105: 19571-8. -
Beamer, C.A. and Holian. A. (2008) Silica suppresses Toll-like receptor ligand-induced dendritic cell activation.
FASEB J. 22: 2053-63. -
Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
J Immunol. 181: 6514-24. -
Hsu, K.M. et al. (2009) Murine cytomegalovirus displays selective infection of cells within hours after systemic administration.
J Gen Virol. 90: 33-43. -
Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
Mol Ther. 17: 65-72. -
Tighe, R.M. et al. (2011) Ozone Inhalation Promotes CX3CR1-Dependent Maturation of Resident Lung Macrophages That Limit Oxidative Stress and Inflammation.
J Immunol. 187: 4800-8. -
Mattsson, J. et al. (2011) Complement activation and complement receptors on follicular dendritic cells are critical for the function of a targeted adjuvant.
J Immunol. 187: 3641-52. -
Fukui Y et al. (2013) Effect of Lactobacillus brevis KB290 on the cell-mediated cytotoxic activity of mouse splenocytes: a DNA microarray analysis.
Br J Nutr. 110 (9): 1617-29. -
Marrella, V. et al. (2015) IL-10 critically modulates B cell responsiveness in Rankl-/- mice.
J Immunol. 194 (9): 4144-53. -
Kolan, S.S. et al. (2015) Lack of non-hematopoietic SIRPα signaling disturbs the splenic marginal zone architecture resulting in accumulation and displacement of marginal zone B cells.
Biochem Biophys Res Commun. 460 (3): 645-50. -
Flores, M> et al. (2015) FcγRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response.
J Immunol. 194 (9): 4240-50. -
Martinez, N. et al. (2016) Impaired Recognition of Mycobacterium tuberculosis by Alveolar Macrophages From Diabetic Mice.
J Infect Dis. 214 (11): 1629-1637. -
Parsa, R. et al. (2016) BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis.
J Exp Med. 213 (8): 1537-53. -
Hayashi, M. et al. (2017) Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines.
EBioMedicine. 15: 127-36. -
Zhao, Z. et al. (2019) Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis.
J Immunol Res. 2019: 9561350. -
Hamasaki, M. et al. (2020) Transcriptional profiling of murine macrophages stimulated with cartilage fragments revealed a strategy for treatment of progressive osteoarthritis.
Sci Rep. 10 (1): 7558. -
Lucas, C.J. et al. (2024) Chikungunya virus infection disrupts lymph node lymphatic endothelial cell composition and function via MARCO.
JCI Insight. 9 (4): e176537. -
Amon, L. et al. (2024) Clec12A, CD301b, and FcγRIIB/III define the heterogeneity of murine DC2s and DC3s.
Cell Rep. 43 (3): 113949. -
Maler, M.D. et al. (2024) Type I Interferon, Induced by Adenovirus or Adenoviral Vector Infection, Regulates the Cytokine Response to Lipopolysaccharide in a Macrophage Type-Specific Manner.
J Innate Immun. 16 (1): 226-47.
- Synonyms
- Scavenger Receptor
- RRID
- AB_322923
- UniProt
- Q60754
- Entrez Gene
- Marco
- GO Terms
- GO:0016021 integral to membrane
- GO:0005044 scavenger receptor activity
- GO:0045087 innate immune response
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