MARCO antibody | ED31

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Rat anti Mouse MARCO

Product Type
Monoclonal Antibody
Clone
ED31
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1849T
Datasheet
C IF WB
SDS
25 µg loader

MCA1849
Datasheet
C IF WB
SDS
0.25 mg loader

Rat anti Mouse MARCO antibody, clone ED31 recognizes the murine cell surface antigen designated MARCO (Macrophage receptor with collagenous structure), which is a member of the class A scavenger receptor family.

MARCO is expressed by distinct populations of macrophages in the spleen and lymph nodes, but is rapidly induced on macrophages in other tissues (e.g. Liver Kupffer cells) during infection or LPS treatment.

Rat anti Mouse MARCO antibody, clone ED31 binds to the C-terminal cysteine rich domain of MARCO, and has been shown to block ligand binding.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
CHO cells expressing mouse MARCO.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q60754
Entrez Gene
Marco
GO Terms
GO:0016021 integral to membrane
GO:0005044 scavenger receptor activity
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of MARCO antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1/200 1/400
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader

Application Based External Images

Functional Assays

Immunofluorescence

Immunohistology - Frozen

Western Blotting

Product Specific References

References for MARCO antibody

  1. van der Laan, L.J. et al. (1999) Regulation and functional involvement of macrophage scavenger receptor MARCO in clearance of bacteria in vivo.
    J Immunol. 162 (2): 939-47.
  2. van der Laan, L.J. et al. (1997) Macrophage scavenger receptor MARCO: in vitro and in vivo regulation and involvement in the anti-bacterial host defense.
    Immunol Lett. 57 (1-3): 203-8.
  3. Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
    J Immunol. 181: 6514-24.
  4. Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
    Mol Ther. 17: 65-72.
  5. Hsu, K.M. et al. (2009) Murine cytomegalovirus displays selective infection of cells within hours after systemic administration.
    J Gen Virol. 90: 33-43.
  6. Kang, Y.S. et al. (2004) The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.
    Proc Natl Acad Sci U S A. 101: 215-20.
  7. Karlsson, M.C. et al. (2003) Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone.
    J Exp Med. 198: 333-40.
  8. Taylor, P.R. et al. (2004) Development of a specific system for targeting protein to metallophilic macrophages.
    Proc Natl Acad Sci U S A. 101: 1963-8.
  9. Alarcón, R. et al. (2005) Expression of scavenger receptors in glial cells. Comparing the adhesion of astrocytes and microglia from neonatal rats to surface-bound beta-amyloid.
    J Biol Chem. 280: 30406-15.
  10. Anthony, R.M. et al. (2008) Identification of a receptor required for the anti-inflammatory activity of IVIG.
    Proc Natl Acad Sci U S A. 105: 19571-8.
  11. Beamer, C.A. and Holian. A. (2008) Silica suppresses Toll-like receptor ligand-induced dendritic cell activation.
    FASEB J. 22: 2053-63.
  12. Granucci, F. et al. (2003) The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia.
    Blood. 102: 2940-7.
  13. Mattsson, J. et al. (2011) Complement activation and complement receptors on follicular dendritic cells are critical for the function of a targeted adjuvant.
    J Immunol. 187: 3641-52.
  14. Fukui Y et al. (2013) Effect of Lactobacillus brevis KB290 on the cell-mediated cytotoxic activity of mouse splenocytes: a DNA microarray analysis.
    Br J Nutr. 110 (9): 1617-29.
  15. Hayashi, M. et al. (2017) Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines.
    EBioMedicine. 15: 127-36.
  16. Martinez, N. et al. (2016) Impaired Recognition of Mycobacterium tuberculosis by Alveolar Macrophages From Diabetic Mice.
    J Infect Dis. 214 (11): 1629-1637.
  17. Parsa, R. et al. (2016) BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis.
    J Exp Med. 213 (8): 1537-53.
  18. Marrella, V. et al. (2015) IL-10 critically modulates B cell responsiveness in Rankl-/- mice.
    J Immunol. 194 (9): 4144-53.
  19. Kolan, S.S. et al. (2015) Lack of non-hematopoietic SIRPα signaling disturbs the splenic marginal zone architecture resulting in accumulation and displacement of marginal zone B cells.
    Biochem Biophys Res Commun. 460 (3): 645-50.
  20. Flores, M> et al. (2015) FcγRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response.
    J Immunol. 194 (9): 4240-50.
  21. Zhao, Z. et al. (2019) Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis.
    J Immunol Res. 2019: 9561350.