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MARCO antibody | ED31

Product Code Applications Pack Size List Price Your Price Qty
MCA1849
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF WB 0.25 mg loader
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loader
MCA1849T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF WB 25 µg loader
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loader

Rat anti Mouse MARCO antibody, clone ED31 recognizes the murine cell surface antigen designated MARCO (Macrophage receptor with collagenous structure), which is a member of the class A scavenger receptor family.

MARCO is expressed by distinct populations of macrophages in the spleen and lymph nodes, but is rapidly induced on macrophages in other tissues (e.g. Liver Kupffer cells) during infection or LPS treatment.

Rat anti Mouse MARCO antibody, clone ED31 binds to the C-terminal cysteine rich domain of MARCO, and has been shown to block ligand binding.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
CHO cells expressing mouse MARCO.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1/200 1/400
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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References for MARCO antibody

  1. van der Laan, L.J. et al. (1997) Macrophage scavenger receptor MARCO: in vitro and in vivo regulation and involvement in the anti-bacterial host defense.
    Immunol Lett. 57 (1-3): 203-8.
  2. van der Laan, L.J. et al. (1999) Regulation and functional involvement of macrophage scavenger receptor MARCO in clearance of bacteria in vivo.
    J Immunol. 162 (2): 939-47.
  3. Whitman, S.C. et al. (2002) Macrophage-specific expression of class A scavenger receptors in LDL receptor(-/-) mice decreases atherosclerosis and changes spleen morphology.
    J Lipid Res. 43: 1201-8.
  4. Granucci, F. et al. (2003) The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia.
    Blood. 102: 2940-7.
  5. Karlsson, M.C. et al. (2003) Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone.
    J Exp Med. 198: 333-40.
  6. Grolleau, A. et al. (2003) Inducible expression of macrophage receptor Marco by dendritic cells following phagocytic uptake of dead cells uncovered by oligonucleotide arrays.
    J Immunol. 171: 2879-88.
  7. Taylor, P.R. et al. (2004) Development of a specific system for targeting protein to metallophilic macrophages.
    Proc Natl Acad Sci U S A. 101: 1963-8.
  8. Kang, Y.S. et al. (2004) The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.
    Proc Natl Acad Sci U S A. 101: 215-20.
  9. View The Latest Product References
  10. Alarcón, R. et al. (2005) Expression of scavenger receptors in glial cells. Comparing the adhesion of astrocytes and microglia from neonatal rats to surface-bound beta-amyloid.
    J Biol Chem. 280: 30406-15.
  11. Anthony, R.M. et al. (2008) Identification of a receptor required for the anti-inflammatory activity of IVIG.
    Proc Natl Acad Sci U S A. 105: 19571-8.
  12. Beamer, C.A. and Holian. A. (2008) Silica suppresses Toll-like receptor ligand-induced dendritic cell activation.
    FASEB J. 22: 2053-63.
  13. Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
    J Immunol. 181: 6514-24.
  14. Hsu, K.M. et al. (2009) Murine cytomegalovirus displays selective infection of cells within hours after systemic administration.
    J Gen Virol. 90: 33-43.
  15. Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
    Mol Ther. 17: 65-72.
  16. Tighe, R.M. et al. (2011) Ozone Inhalation Promotes CX3CR1-Dependent Maturation of Resident Lung Macrophages That Limit Oxidative Stress and Inflammation.
    J Immunol. 187: 4800-8.
  17. Mattsson, J. et al. (2011) Complement activation and complement receptors on follicular dendritic cells are critical for the function of a targeted adjuvant.
    J Immunol. 187: 3641-52.
  18. Fukui Y et al. (2013) Effect of Lactobacillus brevis KB290 on the cell-mediated cytotoxic activity of mouse splenocytes: a DNA microarray analysis.
    Br J Nutr. 110 (9): 1617-29.
  19. Marrella, V. et al. (2015) IL-10 critically modulates B cell responsiveness in Rankl-/- mice.
    J Immunol. 194 (9): 4144-53.
  20. Kolan, S.S. et al. (2015) Lack of non-hematopoietic SIRPα signaling disturbs the splenic marginal zone architecture resulting in accumulation and displacement of marginal zone B cells.
    Biochem Biophys Res Commun. 460 (3): 645-50.
  21. Flores, M> et al. (2015) FcγRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response.
    J Immunol. 194 (9): 4240-50.
  22. Martinez, N. et al. (2016) Impaired Recognition of Mycobacterium tuberculosis by Alveolar Macrophages From Diabetic Mice.
    J Infect Dis. 214 (11): 1629-1637.
  23. Parsa, R. et al. (2016) BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis.
    J Exp Med. 213 (8): 1537-53.
  24. Hayashi, M. et al. (2017) Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines.
    EBioMedicine. 15: 127-36.
  25. Zhao, Z. et al. (2019) Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis.
    J Immunol Res. 2019: 9561350.
  26. Hamasaki, M. et al. (2020) Transcriptional profiling of murine macrophages stimulated with cartilage fragments revealed a strategy for treatment of progressive osteoarthritis.
    Sci Rep. 10 (1): 7558.
  27. Lucas, C.J. et al. (2023) Chikungunya virus infection disrupts lymph node lymphatic endothelial cell composition and function via MARCO.
    bioRxiv. Oct 13 [Epub ahead of print].
  28. Amon, L. et al. (2024) Clec12A, CD301b, and FcγRIIB/III define the heterogeneity of murine DC2s and DC3s.
    Cell Rep. 43 (3): 113949.

Functional Assays

Immunofluorescence

Immunohistology - Frozen

Western Blotting

Synonyms
Scavenger Receptor
RRID
AB_322923
UniProt
Q60754
Entrez Gene
Marco
GO Terms
GO:0016021 integral to membrane
GO:0005044 scavenger receptor activity
GO:0045087 innate immune response

MCA1849

148285 154566 158840 164683

MCA1849T

148286 157680

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