MARCO antibody | ED31
MARCO is expressed by distinct populations of macrophages in the spleen and lymph nodes, but is rapidly induced on macrophages in other tissues (e.g. Liver Kupffer cells) during infection or LPS treatment.
Rat anti Mouse MARCO antibody, clone ED31 binds to the C-terminal cysteine rich domain of MARCO, and has been shown to block ligand binding.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- CHO cells expressing mouse MARCO.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised rats were fused with cells of the mouse SP2/0 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0016021 integral to membrane
- GO:0005044 scavenger receptor activity
- GO:0045087 innate immune response
- For research purposes only
Applications of MARCO antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/200||1/400|
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)||STAR131A||C E P WB||1 ml|
|Goat anti Rat IgG:Biotin (Mouse Adsorbed)||STAR131B||C E IF P WB||0.5 mg|
|Rabbit F(ab')2 anti Rat IgG:Dylight®800||STAR16D800GA||F IF WB||0.1 mg|
|Rabbit F(ab')2 anti Rat IgG:FITC||STAR17B||F||1 mg|
|Rabbit F(ab')2 anti Rat IgG:HRP||STAR21B||C E P RE||1 mg|
|Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)||STAR69||F||0.5 ml|
|Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)||STAR71D800GA||F IF WB||0.1 mg|
|Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)||STAR73||F||0.5 ml|
|Goat anti Rat IgG:HRP (Mouse Adsorbed)||STAR72||C E P||0.5 mg|
Application Based External Images
Immunohistology - Frozen
Product Specific References
References for MARCO antibody
van der Laan, L.J. et al. (1999) Regulation and functional involvement of macrophage scavenger receptor MARCO in clearance of bacteria in vivo.
J Immunol. 162 (2): 939-47.
van der Laan, L.J. et al. (1997) Macrophage scavenger receptor MARCO: in vitro and in vivo regulation and involvement in the anti-bacterial host defense.
Immunol Lett. 57 (1-3): 203-8.
Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
J Immunol. 181: 6514-24.
Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
Mol Ther. 17: 65-72.
Hsu, K.M. et al. (2009) Murine cytomegalovirus displays selective infection of cells within hours after systemic administration.
J Gen Virol. 90: 33-43.
Kang, Y.S. et al. (2004) The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.
Proc Natl Acad Sci U S A. 101: 215-20.
Karlsson, M.C. et al. (2003) Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone.
J Exp Med. 198: 333-40.
Taylor, P.R. et al. (2004) Development of a specific system for targeting protein to metallophilic macrophages.
Proc Natl Acad Sci U S A. 101: 1963-8.
Alarcón, R. et al. (2005) Expression of scavenger receptors in glial cells. Comparing the adhesion of astrocytes and microglia from neonatal rats to surface-bound beta-amyloid.
J Biol Chem. 280: 30406-15.
Anthony, R.M. et al. (2008) Identification of a receptor required for the anti-inflammatory activity of IVIG.
Proc Natl Acad Sci U S A. 105: 19571-8.
Beamer, C.A. and Holian. A. (2008) Silica suppresses Toll-like receptor ligand-induced dendritic cell activation.
FASEB J. 22: 2053-63.
Granucci, F. et al. (2003) The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia.
Blood. 102: 2940-7.
Mattsson, J. et al. (2011) Complement activation and complement receptors on follicular dendritic cells are critical for the function of a targeted adjuvant.
J Immunol. 187: 3641-52.
Fukui Y et al. (2013) Effect of Lactobacillus brevis KB290 on the cell-mediated cytotoxic activity of mouse splenocytes: a DNA microarray analysis.
Br J Nutr. 110 (9): 1617-29.
Hayashi, M. et al. (2017) Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines.
EBioMedicine. 15: 127-36.
Martinez, N. et al. (2016) Impaired Recognition of Mycobacterium tuberculosis by Alveolar Macrophages From Diabetic Mice.
J Infect Dis. 214 (11): 1629-1637.
Parsa, R. et al. (2016) BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis.
J Exp Med. 213 (8): 1537-53.
Marrella, V. et al. (2015) IL-10 critically modulates B cell responsiveness in Rankl-/- mice.
J Immunol. 194 (9): 4144-53.
Kolan, S.S. et al. (2015) Lack of non-hematopoietic SIRPα signaling disturbs the splenic marginal zone architecture resulting in accumulation and displacement of marginal zone B cells.
Biochem Biophys Res Commun. 460 (3): 645-50.
Flores, M> et al. (2015) FcγRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response.
J Immunol. 194 (9): 4240-50.
Zhao, Z. et al. (2019) Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis.
J Immunol Res. 2019: 9561350.