Macrophages/Monocytes antibody | MOMA-2

Rat anti Mouse Macrophages/Monocytes

Product Type
Monoclonal Antibody
Clone
MOMA-2
Isotype
IgG2b
Specificity
Macrophages/Monocytes

Product Code Applications Pack Size List Price Your Price Qty
MCA519G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * IF 0.25 mg loader
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loader
MCA519GT
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SDS Safety Datasheet SDS
C F * IF 25 µg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 recognizes an intracellular antigen of mouse macrophages and monocytes. It reacts strongly with macrophages in lymphoid organs such as tingible body macrophages and macrophages in T cell dependant areas and is extremely useful in immunohistochemistry. Reacts on all mouse strains tested.

Target Species
Mouse
Product Form
Purified IgG - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3).
Immunogen
Mouse lymph node stroma.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Fusion Partners
Spleen cells from immunised Wistar rats were fused with cells of the SP/0 myeloma cell line.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunofluorescence
Immunohistology - Frozen 1/25
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800

Source Reference

  1. Kraal, .G .et al. (1987) Macrophages in T and B cell compartments and other tissue macrophages recognized by monoclonal antibody MOMA-2. An immunohistochemical study.
    Scand J Immunol. 26 (6): 653-61.

References for Macrophages/Monocytes antibody

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  2. Caton, M.L. et al. (2007) Notch-RBP-J signaling controls the homeostasis of CD8- dendritic cells in the spleen.
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  3. Broderick, C. et al. (2002) Constitutive retinal CD200 expression regulates resident microglia and activation state of inflammatory cells during experimental autoimmune uveoretinitis.
    Am J Pathol. 161: 1669-77.
  4. Leung, V.W. et al. (2009) Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice.
    Am J Pathol. 175: 1757-67.
  5. Nix, R.N. et al. (2007) Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.
    PLoS Pathog. 3(12):e193.
  6. Persson, L. et al. (2005) Immunoglobulin treatment reduces atherosclerosis in apolipoprotein E-/- low-density lipoprotein receptor-/- mice via the complement system.
    Clin Exp Immunol. 142: 441-5.
  7. Pillai, M.M. et al. (2009) Inducible transgenes under the control of the hCD68 promoter identifies mouse macrophages with a distribution that differs from the F4/80 - and CSF-1R-expressing populations.
    Exp Hematol. 37: 1387-92.
  8. Wang, Z. et al. (2008) Phospholipase C beta3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice.
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  9. View The Latest Product References
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  11. Fischer, M.B. et al. (1998) Local synthesis of C3 within the splenic lymphoid compartment can reconstitute the impaired immune response in C3-deficient mice.
    J Immunol. 160: 2619-25.
  12. Jiang, H.R. et al. (1999) Macrophages and dendritic cells in IRBP-induced experimental autoimmune uveoretinitis in B10RIII mice.
    Invest Ophthalmol Vis Sci. 40: 3177-85.
  13. Kusunoki, J. et al. (2001) Acyl-CoA:cholesterol acyltransferase inhibition reduces atherosclerosis in apolipoprotein E-deficient mice.
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  14. Lesnik, P. et al. (2003) Decreased atherosclerosis in CX3CR1-/- mice reveals a role for fractalkine in atherogenesis.
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    Mol Cell Biol. 31: 1134-44.
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    Arterioscler Thromb Vasc Biol. 31 (1): 81-5.
  23. Madrigal-Matute, J. et al. (2010) Heat shock protein 90 inhibitors attenuate inflammatory responses in atherosclerosis.
    Cardiovasc Res. 86 (2): 330-7.
  24. de Jager, S.C. et al. (2011) Growth differentiation factor 15 deficiency protects against atherosclerosis by attenuating CCR2-mediated macrophage chemotaxis.
    J Exp Med. 208 (2): 217-25.
  25. Frossard, J.L. et al. (2011) Role of CCL-2, CCR-2 and CCR-4 in cerulein-induced acute pancreatitis and pancreatitis-associated lung injury.
    J Clin Pathol. 64 (5): 387-93.
  26. Bhatia, V.K. et al (2007) Complement C1q reduces early atherosclerosis in low-density lipoprotein receptor-deficient mice.
    Am J Pathol.170: 416-26.
  27. Bourdillon, M.C. et al. (2006) Reduced atherosclerotic lesion size in P-selectin deficient apolipoprotein E-knockout mice fed a chow but not a fat diet.
    J Biomed Biotechnol. 2006 (2): 49193.
  28. Duewell, P. et al. (2010) NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals.
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  29. Weingärtner, O. et al. (2011) Differential effects on inhibition of cholesterol absorption by plant stanol and plant sterol esters in apoE-/- mice.
    Cardiovasc Res. 90: 484-92.
  30. Yamamoto, S. et al. (2011) Oral activated charcoal adsorbent (AST-120) ameliorates extent and instability of atherosclerosis accelerated by kidney disease in apolipoprotein E-deficient mice.
    Nephrol Dial Transplant. 26 (8): 2491-7.
  31. Ng, H.P. et al. (2011) Attenuated atherosclerotic lesions in apoE-Fcγ-chain-deficient hyperlipidemic mouse model is associated with inhibition of Th17 cells and promotion of regulatory T cells.
    J Immunol. 187 (11): 6082-93.
  32. Ruf, M.T. et al. (2012) Chemotherapy-Associated Changes of Histopathological Features of Mycobacterium ulcerans Lesions in a Buruli Ulcer Mouse Model.
    Antimicrob Agents Chemother. 56: 687-96.
  33. Che, J. et al. (2011) Endothelial FGF receptor signaling accelerates atherosclerosis.
    Am J Physiol Heart Circ Physiol. 300: H154-61.
  34. Chen, S. (2010) IL-17A is proatherogenic in high-fat diet-induced and Chlamydia pneumoniae infection-accelerated atherosclerosis in mice.
    J Immunol. 185: 5619-27.
  35. Dieleman, L.A. et al. (1998) Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines.
    Clin Exp Immunol. 114: 385-91.
  36. Gao, Q. et al. (2010) A critical function of Th17 proinflammatory cells in the development of atherosclerotic plaque in mice.
    J Immunol. 185: 5820-7.
  37. Pedersen, T.X. et al. (2010) The pro-inflammatory effect of uraemia overrules the anti-atherogenic potential of immunization with oxidized LDL in apoE-/- mice.
    Nephrol Dial Transplant. 25: 2486-91.
  38. Lee, M.R. et al. (2014) The adipokine Retnla modulates cholesterol homeostasis in hyperlipidemic mice.
    Nat Commun. 5: 4410.
  39. Hoeksema, M.A. et al. (2014) Targeting macrophage Histone deacetylase 3 stabilizes atherosclerotic lesions.
    EMBO Mol Med. 6 (9): 1124-32.
  40. Yamamoto, S. et al. (2015) Atherosclerosis following renal injury is ameliorated by pioglitazone and losartan via macrophage phenotype
    Atherosclerosis. 242 (1): 56-64.
  41. Babaei, S. et al. (2000) Blockade of endothelin receptors markedly reduces atherosclerosis in LDL receptor deficient mice: role of endothelin in macrophage foam cell formation.
    Cardiovasc Res. 2000 Oct;48: 158-67.
  42. Wan W et al. (2015) Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.
    Cardiovasc Res. 106 (3): 478-87.
  43. Krishack, P.A. et al. (2015) Serum Amyloid A Facilitates Early Lesion Development in Ldlr-/- Mice.
    J Am Heart Assoc. 4 (7): pii: e001858.
  44. Aoki, S. et al. (2015) Oral administration of the β-glucan produced by Aureobasidium pullulans ameliorates development of atherosclerosis in apolipoprotein E deficient mice
    Journal Funct Foods. 18: 22-7.
  45. Song, G. et al. (2015) Molecular hydrogen stabilizes atherosclerotic plaque in low-density lipoprotein receptor-knockout mice.
    Free Radic Biol Med. 87: 58-68.
  46. Takata, H. et al. (2015) Vascular angiotensin II type 2 receptor attenuates atherosclerosis via a kinin/NO-dependent mechanism.
    J Renin Angiotensin Aldosterone Syst. 16 (2): 311-20.
  47. Wezel, A. et al. (2015) Mast cells mediate neutrophil recruitment during atherosclerotic plaque progression.
    Atherosclerosis. 241 (2): 289-96.
  48. Oguiza A et al. (2015) Peptide-based inhibition of IκB kinase/nuclear factor-κB pathway protects against diabetes-associated nephropathy and atherosclerosis in a mouse model of type 1 diabetes.
    Diabetologia. 58 (7): 1656-67.
  49. Shuto, Y. et al. (2015) Repetitive Glucose Spikes Accelerate Atherosclerotic Lesion Formation in C57BL/6 Mice.
    PLoS One. 10 (8): e0136840.
  50. Peng, Y. et al. (2016) Inactivation of Semicarbazide-Sensitive Amine Oxidase Stabilizes the Established Atherosclerotic Lesions via Inducing the Phenotypic Switch of Smooth Muscle Cells.
    PLoS One. 11 (4): e0152758.
  51. Hong, Y.F. et al. (2016) Lactobacillus acidophilus K301 Inhibits Atherogenesis via Induction of 24 (S), 25-Epoxycholesterol-Mediated ABCA1 and ABCG1 Production and Cholesterol Efflux in Macrophages.
    PLoS One. 11 (4): e0154302.
  52. Grootaert, M.O. et al. (2016) NecroX-7 reduces necrotic core formation in atherosclerotic plaques of Apoe knockout mice.
    Atherosclerosis. 252: 166-74.
  53. Oguro, A. et al. (2003) NaF induces early differentiation of murine bone marrow cells along the granulocytic pathway but not the monocytic or preosteoclastic pathway in vitro.
    In Vitro Cell Dev Biol Anim. 39 (5-6): 243-8.
  54. van der Sluis, R.J. et al. (2015) Haloperidol inhibits the development of atherosclerotic lesions in LDL receptor knockout mice.
    Br J Pharmacol. 172 (9): 2397-405.
  55. Addison, C.L. et al. (2004) Overexpression of the duffy antigen receptor for chemokines (DARC) by NSCLC tumor cells results in increased tumor necrosis.
    BMC Cancer. 4: 28.
  56. Neele, A.E. et al. (2018) Myeloid Kdm6b deficiency results in advanced atherosclerosis.
    Atherosclerosis. 275: 156-65.
  57. Tang, G. et al. (2019) Metformin inhibited Nod-like receptor protein 3 inflammasomes activation and suppressed diabetes-accelerated atherosclerosis in apoE-/- mice.
    Biomed Pharmacother. 119: 109410.
  58. van Duijn, J. et al. (2019) CD8+ T-cells contribute to lesion stabilization in advanced atherosclerosis by limiting macrophage content and CD4+ T-cell responses.
    Cardiovasc Res. 115 (4): 729-38.
  59. Kato, T. et al. (2020) A non-selective endothelin receptor antagonist bosentan modulates kinetics of bone marrow-derived cells in ameliorating pulmonary hypertension in mice.
    Pulm Circ. 10 (2): 2045894020919355.
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    Cell Rep. 30 (6): 1870-1882.e4.
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    Nat Commun. 11 (1): 6296.
  64. Luque-Martin, R. et al. (2019) Targeting Histone Deacetylases in Myeloid Cells Inhibits Their Maturation and Inflammatory Function With Limited Effects on Atherosclerosis.
    Front Pharmacol. 10: 1242.
  65. Zhao, J. et al. (2019) Atherogenesis in the Carotid Artery with and without Interrupted Blood Flow of Two Hyperlipidemic Mouse Strains.
    J Vasc Res. 56 (5): 241-254.
  66. Burris, R.L. et al. (2020) Maternal exposure to soy diet reduces atheroma in hyperlipidemic F1 offspring mice by promoting macrophage and T cell anti-inflammatory responses.
    Atherosclerosis. 313: 26-34.
  67. Douna, H. et al. (2020) B- and T-lymphocyte attenuator stimulation protects against atherosclerosis by regulating follicular B cells.
    Cardiovasc Res. 116 (2): 295-305.
  68. Sun, Y. et al. (2019) Silencing of junctional adhesion molecule-like protein attenuates atherogenesis and enhances plaque stability in ApoE-/- mice.
    Clin Sci (Lond). 133 (11): 1215-28.
  69. Zhao, J. et al. (2021) Inflammation and enhanced atherogenesis in the carotid artery with altered blood flow in an atherosclerosis-resistant mouse strain.
    Physiol Rep. 9 (11): e14829.
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Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

RRID
AB_321970