Macrophages/Monocytes antibody | MOMA-2
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of macrophages in atherosclerotic aotic lesions from control and ApoE knockout mice.
Image caption:
MOMA-2 staining of ApoE−/− (SKO) and ApoE−/−, P-selectin−/− (DKO) mice fed a chow diet. Macrophages, stained by MOMA-2 monoclonal antibody, are shown binding the endothelial layer and penetrating the intimal lesions in SKO (a, c) and DKO (b, d) animals following, respectively, 15- (a, b) and 20- (c, d) week chow diet. Black arrows show immunostained macrophages. Original magnification: x250.
From: Marie-Claude Bourdillon, Jacques Randon, Lydie Barek, et al..
Reduced Atherosclerotic Lesion Size in -Selectin Deficient Apolipoprotein -Knockout Mice Fed a Chow but Not a Fat Diet ,”
J Biomed Biotechnol, vol. 2006, Article ID 49193, 8 pages, 2006.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of macrophages in adipose tissue of mice using immunohistochemistry.
Image caption:
High number of preadipocyte/macrophage-like cells in the adipose tissue of Nscl-2 (−/−) and Nscl-2 (−/−)×ob/ob compound mutant mice. (A) Number of preadipocyte/macrophage-like cells per 250 adipocytes (F = 250.71; dF = 3; p<10−4; 4 mice per group). (B) Number of MOMA-2 positive but F4/80 negative cells per 250 adipocytes. The difference between MOMA-2 and F4/80 positive cells corresponds to adipocyte precursors (p = 0.038). (C) Richardson-stain of a section of epididymal adipose tissue from 6 months old male double mutant mouse. Arrow indicates groups of preadipocyte/macrophage-like cells between fat cells (scale bar: 25 µm). (D) MOMA-2 staining of paraffin embedded epididymal adipose tissue of a Nscl-2 (−/−) mouse (scale bar: 100 µm). The inlet shows a typical staining of MOMA-2 (scale bar: 15 µm).
From: Ruschke K, Ebelt H, Klöting N, Boettger T, Raum K, Blüher M, et al.. (2009)
Defective Peripheral Nerve Development Is Linked to Abnormal Architecture and Metabolic Activity of Adipose Tissue in Nscl-2 Mutant Mice.
PLoS ONE 4(5): e5516.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519) used for the demonstration of macrophages in aortic atherosclerotic lesions by immunohistochemistry on cryosections.
Image caption:
p210 immunization confers protection against atherosclerosis. Representative pictures of aortic en-face lipid staining from each group shown (A; left panel). Immunization with native p210 resulted in a significant reduction in aortic atherosclerosis when compared to PBS and cBSA/Alum group (A; right panel; n = 9–10 each group). P210 immunization significantly reduced macrophage infiltration (B) assessed by MOMA-2 stain (n = 9–10 each group) and DC presence (C) assessed by CD11c (n = 7–12 each group) stain in aortic sinus plaques. Positive stain indicated by reddish-brown color. Data were analyzed by ANOVA followed by Newman-Keuls multiple group comparison, *P<0.05 vs. PBS and cBSA/alum.
From: Chyu K-Y, Zhao X, Dimayuga PC, Zhou J, Li X, Yano J, et al.. (2012)
CD8+ T Cells Mediate the Athero-Protective Effect of Immunization with an ApoB-100 Peptide.
PLoS ONE 7(2): e30780.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of macrophages in aortic atherosclerotic lesions by immunohistochemistry on formalin fixed cryosections.
Image caption:
PBA treatment influenced the stability of plaque. Representative photomicrographs and the quantitative analysis of smooth muscle cell (SMC), CD3, macrophage (Mφ), and TUNEL in lesion of aortic root of ApoE−/− mice. The aortic root sections were stained with rabbit polyclonal to α- smooth muscle actin, rat anti-mouse macrophage Moma-2 and rat anti mouse CD3. The content of macrophage, smooth muscle cell, CD3 T cell in lesion was analyzed respectively by immunohistochemistry. For detection of cell apoptosis, sections were incubated with anti-TUNEL antibody and the content of apoptotic cells was analyzed by immunofluorescence. (n = 10 per group), *p<0.05, **p<0.01, ***p<0.001.
From: Wang B, Dai S, Dong Z, Sun Y, Song X, Guo C, et al.. (2014)
The Modulation of Endoplasmic Reticulum Stress by Chemical Chaperone Upregulates Immune Negative Cytokine IL-35 in Apolipoprotein E-Deficient Mice.
PLoS ONE 9(1): e87787.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used to evaluate macrophage influx to carotid artery atherosclerotic lesions by immunohistochemistry on formalin fixed cryosections.
Image caption:
Carotid plaque compositions in three groups of mice. A, Representative Sirius-red staining of collagen, Oil-red O staining of lipids, MOMA-2 immunostaining of macrophages and α-SMC-actin immunostaining in three groups of mice; (B) Quantification of staining results in three groups of mice. * P<0.05, ** P<0.01, ***P<0.001 vs. Ad-EGFP group.
From: Liu XL, Zhang PF, Ding SF, Wang Y, Zhang M, Zhao YX, et al.. (2012)
Local Gene Silencing of Monocyte Chemoattractant Protein-1 Prevents Vulnerable Plaque Disruption in Apolipoprotein E-Knockout Mice.
PLoS ONE 7(3): e33497.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of macrophages in atherosclerotic plaques for the aortic sinus by immunohistochemistry on cryosections.
Image caption:
Effects of diet change on atherosclerotic plaques in aortic sinus from apoE-/- mice. (A) Representative photograph of Oil-Red-O and macrophage (MOMA-2) staining of atherosclerotic plaques in the aortic sinus of atherogenic diet (AD) and normal diet (ND) mice. Bar = 0.1 mm. (B) Representative photograph of CD3+ T cell staining of atherosclerotic plaques in the aortic sinus of atherogenic diet (AD) and normal diet (ND) mice. Bar = 0.1 mm. (C) Histomorphometric measurements of each staining are shown in bar graphs. N = 10 in each group for plaque size, lipid content and macrophage content. N = 5 in each group for CD3+ T cell content.
From: Chyu K-Y, Lio WM, Dimayuga PC, Zhou J, Zhao X, Yano J, et al.. (2014)
Cholesterol Lowering Modulates T Cell Function In Vivo and In Vitro. PLoS ONE 9(3): e92095.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519) used for the detection of macrophages in atherosclerotic plaques by immunohistochemistry on cryosections.
Image caption:
Lesion complexity does not differ with CCL3 bone marrow genotype. LDLR−/− mice were transplanted with C57BL/6 or CCL3−/− bone marrow. At four weeks post-BMT, mice were placed on WD for 12 weeks. ORO (4×), Trichrome, MOMA-2, and CD4 stained lesion (10×) are in columns as indicated on figure. Gender and donor marrow are indicated on the left of the figure. Sections were chosen from images representing mice with lesion areas close to the mean of their respective groups.
From: Kennedy A, Gruen ML, Gutierrez DA, Surmi BK, Orr JS, Webb CD, et al.. (2012)
Impact of Macrophage Inflammatory Protein-1α Deficiency on Atherosclerotic Lesion Formation, Hepatic Steatosis, and Adipose Tissue Expansion.
PLoS ONE 7(2): e31508.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of macrophages in aortic sinus lesions using immunohistochemistry on formalin fixed cryosections.
Image caption:
The quantification of lesion area for macrophage abundance as calculated using Moma-positive staining.
From: Wang L, Yang M, Arias A, Song L, Li F, Tian F, et al. (2015) Splenocytes Seed Bone Marrow of Myeloablated Mice: Implication for Atherosclerosis.
PLoS ONE 10(6): e0125961.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
AlexaFluor®488 conjugated Rat anti Mouse macrophages and monocyte antibody, clone MOMA-2 (MCA519A488) used for the evaluation of macrophage antigen expression in vitro by immunofluorescence.
Image caption:
Inhibition of HDAC activity alters the phenotype of macrophages generated after 11 days of culture. (A-H) Immunocytochemistry of activated-macrophage markers, F4/80 and Moma-2. Pancake-like shape macrophages generated in the GM-CSF group are immunopositive for F4/80 (A and C) and Moma-2 (E and G). In the atypical elongated macrophages generated in the TSA + GM-CSF, the F4/80 and Moma-2 are down regulated (B, D, F and H—arrow). (I-P) Immunocitochemistry of M1 macrophage marker iNOS, and M2 macrophage marker, arginase 1. In the GM-CSF group, macrophages with pancake-like shape are immunopositive for iNOS (I and K) and for arginase 1 (M and O). The atypical elongated macrophage generated in the TSA + GM-CSF group are immunopositive for iNOS (J and L—arrow) and for arginase 1 (N and P—asterisk). Arginase 1 correlates to round cells in the GM-CSF group (M and O, asterisk) while in the TSA + GM-CSF group, the arginase 1 is associated only to elongated macrophages (N and P—asterisk).
From: Cabanel M, Brand C, Oliveira-Nunes MC, Cabral-Piccin MP, Lopes MF, Brito JM, et al. (2015) Epigenetic Control of Macrophage Shape Transition towards an Atypical Elongated Phenotype by Histone Deacetylase Activity. PLoS ONE 10(7): e0132984.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse macrophages and monocytes antibody, clone MOMA-2 (MCA519G) used for the demonstration of infiltrating macrophages in mouse retina by immunohistochemistry on cryosections.
Image caption:
Monocyte/Macrophage infiltration in sections from C57BL/6 and S100B KO mice. A, C57BL/6 WT retinal section from mouse with EAU showing cells staining positively for MOMA-2 (red). B, positive MOMA-2 staining in S100B KO with EAU was also observed in the rod outer segments and vitreous. No positively stained cells were observed in negative control staining in C57BL/6 mice (C) or S100B KO mice with EAU (D), where primary antibody was replaced with TBS. No positive staining was observed in naïve C57BL/6 WT (E) or S100B KO mice (F). Cells positive for MOMA-2 were counted in a total area of 1.5 mm2. An average cell number was calculated from 3 sections taken at spaced intervals through the eye. A significant reduction in positively stained cells was observed in S100B KO mice compared to C57BL/6 WT mice both in the retinal layers overall (G) and specifically within the rod outer segments (ROS) (H). Error bars indicate SEM; n = 8 randomly selected eyes per genotype. Scale bars indicate 100 μm.
From: Niven J, Hoare J, McGowan D, Devarajan G, Itohara S, Gannagé M, et al. (2015) S100B Up-Regulates Macrophage Production of IL1β and CCL22 and Influences Severity of Retinal Inflammation.
PLoS ONE 10(7): e0132688.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of mouse macrophages in atherosclerotic lesions by immunohistochemistry on cryosections.
Image caption:
Atherosclerotic lesions in aortic sinus. (A,B) Representative images of oil red O-stained atherosclerotic lesions in aortic sinus. (C,D) Immunohistochemical staining of macrophages (brown) in the cross-sections adjacent to the oil red O-stained images. (E) Quantitative analysis of the lesion area. Each dot indicates mean size of the oil red O-stained lesion area in each mouse. Bars indicate mean ± SEM of 8 mice for each group.
From: Shuto Y, Asai A, Nagao M, Sugihara H, Oikawa S (2015)
Repetitive Glucose Spikes Accelerate Atherosclerotic Lesion Formation in C57BL/6 Mice.
PLoS ONE 10(8): e0136840.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the identification of infiltrating macrophages in atherosclerotic lesions by immunohistochemistry on formalin fixed cryosections.
Image caption:
SSAO inactivation stabilized the established atherosclerotic lesions under hypercholesterolemia.
Female LDLr KO mice were fed WTD for 6 weeks to induce the formation of atherosclerotic lesions. Thereafter, 0.125% semicarbazide were added in drinking water to inactivate SSAO during the subsequent 3 weeks (A) before the analysis of SSAO activities in the visceral adipose tissue (B), plasma cholesterol levels (C) and atherosclerotic lesions at the aortic root (D-G). (D) A scattered dot plot (left) or representative photomicrographs showing the size of atherosclerotic lesions. Sections were stained with oil-red-O (original magnification 40x, right panel). Each dot represents the mean lesion area of a single mouse, and the horizontal bar indicates the mean value of the group (left panel). (E) Representative photomicrographs showing macrophages and collagens in lesions. Sections of aortic roots were stained with antibodies against Moma-2 to visualize macrophages (brown, 100x) or with Masson’s Trichrome Accustain to visualize collagens (blue, 100x). (F & G) Bar graphs showing the lesion contents of macrophages (F), collagens (G), necrotic core (H) and cap thickness (I). Results were expressed as mean±SEM. Statistically significant difference *p<0.05, **p<0.01, and ***p<0.001 vs vehicle.
From: Peng Y, Wang J, Zhang M, Niu P, Yang M, Yang Y, et al. (2016)
Inactivation of Semicarbazide-Sensitive Amine Oxidase Stabilizes the Established Atherosclerotic Lesions via Inducing the Phenotypic Switch of Smooth Muscle Cells.
PLoS ONE 11(4): e0152758.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the identification of infiltrating macrophages in atherosclerotic lesions by immunohistochemistry on formalin fixed cryosections.
Image caption:
SSAO inactivation stabilized the established atherosclerotic lesions after normalization of hypercholesterolemia by diet lipid lowering.
Male LDLr KO mice were fed WTD for 9 weeks to induce the formation of atherosclerotic lesions. Thereafter, these animals were fed regular chow diet to normalize hypercholesterolemia (A). Drinking water containing 0.125% semicarbazide was given to these chow-fed animals in the subsequent 6 weeks (A) before the analysis of SSAO activities in the visceral adipose tissue (B), plasma cholesterol levels (C) or atherosclerotic lesions at the aortic root (D-G). (D) A scattered dot plot (left) or representative photomicrographs showing the size of atherosclerotic lesions. Sections were stained with oil-red-O (original magnification 40x, right panel). Each dot represents the mean lesion area of a single mouse, and the horizontal bar indicates the mean value of the group (left panel). (E) Representative photomicrographs showing the lesion contents of Moma-2+ macrophages (upper panel) or collagens (lower panel) in each group. Sections of aortic roots were stained with antibodies against Moma-2 to visualize macrophages (brown, 100x) or with Masson’s Trichrome Accustain to visualize collagens (blue, 100x). (F & G) Bar graphs showing the lesion contents of macrophages (F), collagens (G), necrotic core (H), and cap thickness (I) in vehicle- or semicarbazide-treated mice. Results were expressed as mean ±SEM. Statistically significant difference ***p<0.001 and *p<0.05 vs vehicle.
From: Peng Y, Wang J, Zhang M, Niu P, Yang M, Yang Y, et al. (2016)
Inactivation of Semicarbazide-Sensitive Amine Oxidase Stabilizes the Established Atherosclerotic Lesions via Inducing the Phenotypic Switch of Smooth Muscle Cells.
PLoS ONE 11(4): e0152758.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse monocytes/macrophages antibody, clone MOMA-2 (MCA519G) used for the identification of infiltrating macrophages in a mouse model of Buruli ulcer by immunohistochemistry in formalin fixed paraffin embedded tissue sections along with Rat anti Mouse CD45R, clone RA3-6B2 (MCA1258G) to identify B cells and Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) to identify T-cells. Neutrophils are stained using an anti neutrophil monoclonal antibody.
Image caption:
Infiltration of leukocytes within the footpad infected with M. ulcerans.
Immunohistochemistry was performed on 5 micrometer paraffin embedded sections of footpad of mice infected with a suspension of M. ulcerans (NM20/02) in order to identify the nature of the leukocytic infiltrate present within and around the bacterial foci. B cells were stained with anti-CD45R antibody (A), T cells with anti-CD3 antibody (B), Macrophages with MOMA (C) and neutrophils with anti-Ly6G/Ly6C (D).
From: Bénard A, Sala C, Pluschke G (2016)
Mycobacterium ulcerans Mouse Model Refinement for Pre-Clinical Profiling of Vaccine Candidates.
PLoS ONE 11(11): e0167059.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used to identify macrophages in atherosclerotic plaques within the aortic roots of mice by immunofluorescence on cryostat sections.
Image caption:
Acly deletion in macrophages elicits a favorable plaque phenotype.
Representative immunohistochemical analysis of macrophages (MOMA-2) and TGF-β in mouse plaques from control or AclyM-KO mice (n = 6/6 ctrl/KO from one experiment). Scale bar represents 100 μm. n Quantification of colocalization, percentage of TGF-β+ area in MOMA-2+ area.
From: Baardman J, et al.
Macrophage ATP citrate lyase deficiency stabilizes atherosclerotic plaques.
Nat Commun. 2020 Dec 8;11(1):6296.
doi: 10.1038/s41467-020-20141-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of macrophages in mouse liver by immunofluorescence on formalin fixed tissue sections.
Image caption:
S. typhimurium reside in macrophages that appear to Have multiple nuclei. Confocal fluorescence microscopy of 50-μm-thick liver sections from a 1-wk-infected Slc11a1 (Nramp1) wild-type mouse. S. typhimurium (O-antigen, arrows) are red, macrophages (F4–80 and MOMA-2) are blue, DNA (DAPI) is gray, and phalloidin is green. (A) Low power image, scale bar is 40 μm. (B–H) Montage of 4-μm optical sections through the boxed region of (A). Scale bar is 20 μm. (I and J) Enlarged images showing actin rings (arrowheads) around nuclei within the multinucleate macrophage. The endogenous macrophage nucleus is visible in (I) and is labeled with an N.
From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.
PLoS Pathog 3(12): e193.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of macrophages in mouse liver by immunofluorescence on formalin fixed tissue sections.
Image caption:
S. Typhimurium-Infected macrophages containing phagocytosed neutrophils and T Cells Confocal fluorescence microscopy of 50-μm-thick liver sections from 1-wk-infected Slc11a1 wild-type mice. (A–C) S. Typhimurium (O-antigen, arrows) are red, macrophages (F4–80 and MOMA-2) are blue, DNA (DAPI) is gray, phalloidin is green, and neutrophils (Gr-1/Ly-6G/RB6-8C5) are pink (arrowheads). (A) Collapsed image from a 40-μm Z-stack. Scale bar is 20 μm. (B and C) Sections from (A) that are 4 μm apart. (D–G) T cells within multinucleate macrophages. Macrophages (F4–80 and MOMA-2) are blue (D, G, and H), T cells (CD3zeta) are red (D, G, arrowheads), DAPI is gray (E, G), actin-bound phalloidin is green (F, G). (G) Is a composite of (D, E, and F). Scale bars are 16 μm. (H) An image from a different mouse stained and labeled as described for (D–G). Scale bar is 8 μm.
From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.
PLoS Pathog 3(12): e193.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of bone marrow derived macrophages in mouse by immunofluorescence.
Image caption:
Activated bone marrow–derived macrophages (BMDMs) phagocytose live host cells. Confocal fluorescence microscopy. BMDMs are blue (F4–80 and MOMA-2), human T cells (Jurkats) are green (CMFDA-stained), and DNA is gray (DAPI). (A–C) 30 min after the addition of Jurkats to BMDMs. (A) Unactivated BMDMs show little association with Jurkat cells, many of which were washed away. Scale bar is 40 μm (B and C). Activated macrophages phagocytose Jurkat cells; arrow denotes engulfed cell, arrowheads show partially engulfed cells. Scale bars are 40 μm (B) and 8 μm (C). (D–F) 42 h after mock-infection (D), or S. Typhimurium-infection (O-antigen, red, arrows, E and F). Scale bars are 20 μm (D), 40 μm (E), and 16 μm (F)..
From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.
PLoS Pathog 3(12): e193.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used for the detection of macrophages in aortic atherosclerotic lesions by immunohistochemistry on cryosections.
Image caption:
Representative sections with immunohistochemical analysis. Apo E-knockout mice were fed a Western-type diet and treated with PBS (control group) (n = 10) or 1 mg/kg/day 15d-PGJ2 (15d-PGJ2 group) (n = 10) for 2 mo. Representative cross-sections of the aortic sinus were stained with MOMA-2 (A), which detected macrophages, and MCP-1 Abs (B), MIF Abs (C), TNF-α Abs (D), MMP-9 Abs (E), PPARγ Abs (F), and counterstained with hematoxylin. Right sections are control group and left ones are 15d-PGJ2 group in each figure. Black arrows indicate the positive lesions.
From: Seno T, Hamaguchi M, Ashihara E, Kohno M, Ishino H, Yamamoto A, et al. (2011) 15-Deoxy-Δ12,14 Prostaglandin J2 Reduces the Formation of Atherosclerotic Lesions in Apolipoprotein E Knockout Mice. PLoS ONE 6(10): e25541.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA519G) used to immunostain RAW 264.7 cells using immunofluorescence.
Image caption:
Immunofluorescence and western blot analysis of MMP-2 and MMP-9 expression in vitro. Panel A shows the immunofluorescence images of MMP-2 in macrophages receiving doxycycline, simvastatin or no treatment. Panel B shows the immunofluorescence images of MMP-9 in macrophages receiving doxycycline, simvastatin or no treatment. Panel C shows western blot analysis of MMP-2 and MMP-9 expression. Panel D and E are the quantitative analysis of Panel C. Blue color represents DAPI staining, green color MOMA-2 staining and red color MMP-2 or MMP-9 staining. Bars = 20 μm. Group D: doxycycline-treated group; Group S: simvastatin-treated group; Control: control group. **P<0.01, vs. Control group.
From: Dong M, Zhong L, Chen WQ, Ji XP, Zhang M, Zhao YX, et al. (2012) Doxycycline Stabilizes Vulnerable Plaque via Inhibiting Matrix Metalloproteinases and Attenuating Inflammation in Rabbits.
PLoS ONE 7(6): e39695.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse macrophages/monocytes antibody, clone MOMA-2 (MOMA-2) used to stain macrophages in atherosclerotic lesions in mouse aortas by immunohistochemistry on cryosections.
Image caption:
Reduced monocyte recruitment and inflammation in the aortas of ApoE−/− mice with inducible deletion of NEMO in smooth muscle cells. Male NEMOSMCiKO/ApoE−/− mice and ApoE−/− mice were fed a HFD for 10 weeks before sacrifice. (a) Representative pictures from immunostainings of atherosclerotic lesions of ApoE−/− or NEMOSMCiKO/ApoE−/− mice with antibodies against MOMA-2. Scale bars = 0.1 mm.
From: Imai T, Van TM, Pasparakis M, Polykratis A.
Smooth muscle cell specific NEMO deficiency inhibits atherosclerosis in ApoE-/- mice.
Sci Rep. 2022 Jul 22;12(1):12538.
doi: 10.1038/s41598-022-16737-8.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse dectin-1 antibody, clone 2A11 (MCA2289) used to label macrophages in mouse carotid tissues by immunohistochemistry on cryosections.
Image caption:
Immunohistochemical analysis of lesion formation in ligated carotid arteries of B6 and BALB Apoe−/− mice with different durations of ligation. Sections were stained with antibodies against macrophages Arrows point at stained cells. Original magnification: ×40.
From: Zhao J, Huangfu C, Chang Z, Zhou W, Grainger AT, Liu Z, Shi W.
Inflammation and enhanced atherogenesis in the carotid artery with altered blood flow in an atherosclerosis-resistant mouse strain.
Physiol Rep. 2021 Jun;9(11):e14829.
doi: 10.14814/phy2.14829.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 (MCA947G) used to label macrophages in atherosclerotic plaques by immunohistochemistry on cryostat sections.
Image caption:
PD-1 stimulation inhibits atherosclerosis development. Macrophage content was determined using MOMA-2 antibody staining, and is shown as total MOMA-2 positive area, as well as the percentage of total plaque (C). Scale bars indicate 250 μm. Mean ± SEM are shown. Statistics was performed using unpaired T-test. P ≤0.05 are considered significant. *p ≤0.05.
From: Grievink HW, Smit V, Verwilligen RAF, Bernabé Kleijn MNA, Smeets D, Binder CJ, Yagita H, Moerland M, Kuiper J, Bot I, Foks AC.
Stimulation of the PD-1 Pathway Decreases Atherosclerotic Lesion Development in Ldlr Deficient Mice.
Front Cardiovasc Med. 2021 Nov 1;8:740531.
doi: 10.3389/fcvm.2021.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/monocytes antibody, clone MOMA-2 (MCA519G) used to label macrophages in murine atherosclerotic lesions by immubohistochemistry on formalin fixed cryosections.
Image caption:
Adoptive transfer of IFNγ-B protects against atherosclerosis. ApoE−/− mice were fed a Western type diet for 7 weeks. After 2 weeks they received a perivascular collar and were treated with PBS, freshly isolated B cells (B cells) or B cells stimulated with 20.0 ng/ml IFNγ for 24 h (IFNγ-B cells). Mice received a total of three injections and injections were spaced every two weeks. After 7 weeks, mice were sacrificed and (C) Macrophage content was analyzed using a MOMA-2 staining. Data are analyzed with a One-Way ANOVA and shown as mean ± SEM (*p <0.05, **p <0.01). n = 8–12/group.
From: Douna H, de Mol J, Amersfoort J, Schaftenaar FH, Kiss MG, Suur BE, Kroner MJ, Binder CJ, Bot I, Van Puijvelde GHM, Kuiper J, Foks AC.
IFNγ-Stimulated B Cells Inhibit T Follicular Helper Cells and Protect Against Atherosclerosis.
Front Cardiovasc Med. 2022 Feb 2;9:781436.
doi: 10.3389/fcvm.2022.781436.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Macrophages/Monocytes
- Product Type
- Monoclonal Antibody
- Clone
- MOMA-2
- Isotype
- IgG2b
- Specificity
- Macrophages/Monocytes
Antibody Quality and Performance Guarantee
| Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 recognizes an intracellular antigen of mouse macrophages and monocytes. It reacts strongly with macrophages in lymphoid organs such as tingible body macrophages and macrophages in T cell dependant areas and is extremely useful in immunohistochemistry. Reacts on all mouse strains tested. |
- Target Species
- Mouse
- Product Form
- MCA519GT: Purified IgG - liquid
- MCA519G: Purified IgG - liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3).
- Immunogen
- Mouse lymph node stroma.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Fusion Partners
- Spleen cells from immunized Wistar rats were fused with cells of the SP/0 myeloma cell line.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
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- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Source Reference
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Kraal, .G .et al. (1987) Macrophages in T and B cell compartments and other tissue macrophages recognized by monoclonal antibody MOMA-2. An immunohistochemical study.
Scand J Immunol. 26 (6): 653-61.
References for Macrophages/Monocytes antibody
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Gal, A. et al. (1996) Nitric oxide production in SJL mice bearing the RcsX lymphoma: a model for in vivo toxicological evaluation of NO.
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Fischer, M.B. et al. (1998) Local synthesis of C3 within the splenic lymphoid compartment can reconstitute the impaired immune response in C3-deficient mice.
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Jiang, H.R. et al. (1999) Macrophages and dendritic cells in IRBP-induced experimental autoimmune uveoretinitis in B10RIII mice.
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Kusunoki, J. et al. (2001) Acyl-CoA:cholesterol acyltransferase inhibition reduces atherosclerosis in apolipoprotein E-deficient mice.
Circulation. 103: 2604-9. -
Lesnik, P. et al. (2003) Decreased atherosclerosis in CX3CR1-/- mice reveals a role for fractalkine in atherogenesis.
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View The Latest Product References
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Krishack, P.A. et al. (2015) Serum Amyloid A Facilitates Early Lesion Development in Ldlr-/- Mice.
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