Ly-6C antibody | ER-MP20
Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MCA2389GA) used for the evaluation of Ly-6C expression on inflammatory macrophages in a brain injury model by flow cytometry.
Image caption:
Dual targeting of CCR2/5 with CVC mitigates TBI-induced macrophage recruitment. a In WT mice, CVC or vehicle was administered BID via oral gavage at 100 mg/kg at 2 and 10 h post surgery before animals were euthanized for various endpoints at 24 h following surgery. b A cohort of WT aged TBI animals (n = 8/group) was used for flow cytometry analysis of macrophage infiltration into the injured brain. Twenty-four hours after injury, there was a significant decrease in the number of peripheral macrophages (CD11b+F4/80+CD45hiLy6C+) in the CVC-treated animals compared to their vehicle-treated counterparts
From: Morganti JM, Riparip LK, Chou A, Liu S, Gupta N, Rosi S.
Age exacerbates the CCR2/5-mediated neuroinflammatory response to traumatic brain injury.
J Neuroinflammation. 2016 Apr 18;13(1):80.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
PE conjugated Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MCA2389PE) used for the identification of osteoclast precursor populations by flow cytometry in conjunction with Rat anti Mouse CD31 antibody, clone ER-MP12 (MCA2388B).
Image caption:
Identification of different subsets of osteoclast precursors in bone marrow from long bone, calvaria, vertebra and jaw in WT and Il1rn-/-mice.
Cells were labeled with anti-CD31 and -Ly-6C and osteoclast precursor subsets were gated as: early blasts (CD31hi Ly-6C-), myeloid blasts (CD31+ Ly-6C+), and monocytes (CD31- Ly-6Chi) as encircled in each profile. Other populations mainly contained lymphocytes (Labeled A), erythroid blasts (Labeled B) and granulocytes (Labeled C).
From: Ascone G, Cao Y, Jansen IDC, Di Ceglie I, van den Bosch MHJ, Blom AB, van Lent PLEM, Everts V, de Vries TJ.
Increase in the Number of Bone Marrow Osteoclast Precursors at Different Skeletal Sites, Particularly in Long Bone and Jaw Marrow in Mice Lacking IL-1RA.
Int J Mol Sci. 2020 May 27;21(11):3774.
doi: 10.3390/ijms21113774.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MCA2389GA) used to define myeloid subsets by flow cytometry
Image caption:
Representative FACS plot for the identification of lymphoid and myeloid subsets in Ldlr-/- mice. (A) To identify lymphoid cells, B-cells (CD19+) were excluded from innate immune cells (CD45+), T-cells (CD19+ and CD3+) were further subdivided into cytotoxic T-cells (CD8) and T-helper cells (CD4+). To identify myeloid cells, (B) neutrophils (Ly6G+) were excluded from innate immune cells (CD45+) and non-neutrophils (Ly6G and SiglecF-) cells were further subdivided into monocytes (CD11b+). Monocytes were then classified into pro-inflammatory (Ly6Chigh), intermediate (Ly6Cmedium) and anti-inflammatory (Ly6Clow) monocytes.
From: Thiem K, Hoeke G, van den Berg S, Hijmans A, Jacobs CWM, Zhou E, Mol IM, Mouktaroudi M, Bussink J, Kanneganti TD, Lutgens E, Stienstra R, Tack CJ, Netea MG, Rensen PCN, Berbée JFP, van Diepen JA.
Deletion of hematopoietic Dectin-2 or CARD9 does not protect against atherosclerotic plaque formation in hyperlipidemic mice.
Sci Rep. 2019 Mar 13;9(1):4337.
doi: 10.1038/s41598-019-40663-x.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
C F ResetRat anti Mouse Ly-6C
- Product Type
- Monoclonal Antibody
- Clone
- ER-MP20
- Isotype
- IgG2a
- Specificity
- Ly-6C
Antibody Quality and Performance Guarantee
| Rat anti Mouse Ly-6C antibody, clone ER-MP20 recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes. Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state. Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990). Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Balb/c macrophage precursor cell hybrids.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| Description | Rat IgG2a Negative Control | ||||||
References for Ly-6C antibody
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Zhang, Y. & Bliska, J.B. (2010) YopJ-promoted cytotoxicity and systemic colonization are associated with high levels of murine interleukin-18, gamma interferon, and neutrophils in a live vaccine model of Yersinia pseudotuberculosis infection.
Infect Immun 78: 2329-41. -
Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
Eur J Immunol. 20 (1): 27-34. -
de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.
J Immunol Methods. 217 (1-2): 27-39. -
Schatteman, G.C. et al. (2010) Lin- Cells Mediate Tissue Repair by Regulating MCP-1/CCL-2.
Am J Pathol. 177: 2002-10. -
Baumeister, T. et al. (2003) Interleukin-3Ralpha+ myeloid dendritic cells and mast cells develop simultaneously from different bone marrow precursors in cultures with interleukin-3.
J Invest Dermatol. 121: 280-8. -
Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
Mol Ther. 17: 65-72. -
Nikolic, T. et al. (2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.
Int Immunol. 15: 515-24. -
Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
Am J Pathol. 158 (1): 131-45.
View The Latest Product References
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Lesokhin, A.M. et al. (2012) Monocytic CCR2+ Myeloid-Derived Suppressor Cells Promote Immune Escape by Limiting Activated CD8 T-cell Infiltration into the Tumor Microenvironment.
Cancer Res. 72: 876-86. -
Chan, J. et al. (1998) Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression.
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Bossaller, L. et al. (2013) Overexpression of membrane-bound fas ligand (CD95L) exacerbates autoimmune disease and renal pathology in pristane-induced lupus.
J Immunol. 191: 2104-14. -
Garcia, J.A. et al. (2013) Regulation of adaptive immunity by the fractalkine receptor during autoimmune inflammation.
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Stijlemans, B. et al. (2015) Murine Liver Myeloid Cell Isolation Protocol
BIO-PROTOCOL. 5 (10). -
Damya, L. et al. (2014) Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)
BIO-PROTOCOL. 4 (22). -
Morganti, J.M. et al. (2016) Age exacerbates the CCR2/5-mediated neuroinflammatory response to traumatic brain injury.
J Neuroinflammation. 13 (1): 80. -
Mooney, J.E. et al. (2010) Cellular plasticity of inflammatory myeloid cells in the peritoneal foreign body response.
Am J Pathol. 176 (1): 369-80. -
Iwasaki, Y. et al. (2011) In situ proliferation and differentiation of macrophages in dental pulp.
Cell Tissue Res. 346 (1): 99-109. -
Movahedi, K. et al. (2012) Nanobody-based targeting of the macrophage mannose receptor for effective in vivo imaging of tumor-associated macrophages.
Cancer Res. 72 (16): 4165-77. -
Ribechini, E. et al. (2009) Gr-1 antibody induces STAT signaling, macrophage marker expression and abrogation of myeloid-derived suppressor cell activity in BM cells.
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Bossaller, L. et al. (2016) TLR9 Deficiency Leads to Accelerated Renal Disease and Myeloid Lineage Abnormalities in Pristane-Induced Murine Lupus.
J Immunol. 197 (4): 1044-53. -
Barnes, M.A. et al. (2015) Macrophage migration inhibitory factor is required for recruitment of scar-associated macrophages during liver fibrosis.
J Leukoc Biol. 97 (1): 161-9. -
Ohnishi, K. et al. (2012) Immunohistochemical detection of possible cellular origin of hepatic histiocytic sarcoma in mice.
J Clin Exp Hematop. 52 (3): 171-7. -
Van den Bossche. J. et al. (2012) Claudin-1, claudin-2 and claudin-11 genes differentially associate with distinct types of anti-inflammatory macrophages in vitro and with parasite- and tumour-elicited macrophages in vivo.
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Houthuys, E. et al. (2010) A method for the isolation and purification of mouse peripheral blood monocytes.
J Immunol Methods. 359 (1-2): 1-10. -
Greifenberg, V. et al. (2009) Myeloid-derived suppressor cell activation by combined LPS and IFN-gamma treatment impairs DC development.
Eur J Immunol. 39 (10): 2865-76. -
Cardona, S.M. et al. (2015) Disruption of Fractalkine Signaling Leads to Microglial Activation and Neuronal Damage in the Diabetic Retina.
ASN Neuro. 7 (5):1759091415608204. -
Waddell, A. et al. (2011) Colonic eosinophilic inflammation in experimental colitis is mediated by Ly6C(high) CCR2(+) inflammatory monocyte/macrophage-derived CCL11.
J Immunol. 186 (10): 5993-6003. -
Robbie, S.J. et al. (2016) Enhanced Ccl2-Ccr2 signaling drives more severe choroidal neovascularization with aging.
Neurobiol Aging. 40: 110-9. -
Cao, Y. et al. (2016) IL-1β differently stimulates proliferation and multinucleation of distinct mouse bone marrow osteoclast precursor subsets.
J Leukoc Biol. 100 (3): 513-23. -
Cao, Y. et al. (2017) TNF-α has both stimulatory and inhibitory effects on mouse monocyte-derived osteoclastogenesis.
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PLoS One. 12 (9): e0183741. -
Koohy, H. et al. (2018) Genome organization and chromatin analysis identify transcriptional downregulation of insulin-like growth factor signaling as a hallmark of aging in developing B cells.
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Pluijmert, N.J. et al. (2020) Effects on cardiac function, remodeling and inflammation following myocardial ischemia-reperfusion injury or unreperfused myocardial infarction in hypercholesterolemic APOE*3-Leiden mice.
Sci Rep. 10 (1): 16601. -
Ascone, G. et al. (2020) Increase in the Number of Bone Marrow Osteoclast Precursors at Different Skeletal Sites, Particularly in Long Bone and Jaw Marrow in Mice Lacking IL-1RA.
Int J Mol Sci. 21 (11): 3774. -
Pluijmert, N.J. et al. (2021) Phosphorylcholine antibodies restrict infarct size and left ventricular remodelling by attenuating the unreperfused post-ischaemic inflammatory response.
J Cell Mol Med. 25 (16): 7772-82. -
Njock, M-K. (2022) Endothelial extracellular vesicles promote tumour growth by tumour-associated macrophage reprogramming
J Extracell Vesicles 2022 Jun;11(6):e12228. -
Vainchtein, I.D. et al. (2023) Characterizing microglial gene expression in a model of secondary progressive multiple sclerosis.
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Mielczarek, O. et al. (2023) Intra- and interchromosomal contact mapping reveals the Igh locus has extensive conformational heterogeneity and interacts with B-lineage genes.
Cell Rep 42 (9):113074. -
Thiem, K. et al. (2019) Deletion of hematopoietic Dectin-2 or CARD9 does not protect against atherosclerotic plaque formation in hyperlipidemic mice.
Sci Rep. 9 (1): 4337. -
Heissig, B. et al. (2022) siRNA against CD40 delivered via a fungal recognition receptor ameliorates murine acute graft-versus-host disease.
EJHaem. 3 (3): 849-61. -
Cardona, S.M. et al. (2018) Role of the Fractalkine Receptor in CNS Autoimmune Inflammation: New Approach Utilizing a Mouse Model Expressing the Human CX3CR1(I249/M280) Variant.
Front Cell Neurosci. 12: 365. -
Burzynski, L.C. et al. (2023) Thrombin-activated interleukin-1α drives atherogenesis, but also promotes vascular smooth muscle cell proliferation and collagen production.
Cardiovasc Res. 119 (12): 2179-89. -
Story, M.E. et al. (2024) Resident memory T cells in dirty mice suppress innate cell activation and infiltration into the skin following stimulation with alarmins
BioRχiv 16 Jul [Epub ahead of print]. -
Lepland, A. et al. (2024) Therapeutic Tumor Macrophage Reprogramming in Breast Cancer Through a Peptide-Drug Conjugate
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- Synonyms
- Lymphocyte Antigen 6C2
- RRID
- AB_1102741
- UniProt
- P0CW03
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