JAM-C antibody | CRAM-18 F26
Rat anti Mouse JAM-C antibody, clone CRAM-18 F26 (MCA5935) used to demonstrate JAM-C expression in human chorionic vessels and syncitiotrophoblasts by immunofluorescence.
Image caption:
JAM-C expression was abundant in chorionic vessels but scarce in the STB layer. (a) Frozen sections of human placentae derived from women infected or not (control) with ZIKV were processed for immunofluorescence with a rat antibody anti JAM-C and a mouse antibody anti cytokeratin 18 (K18). Nuclei were stained with DAPI. Fetal endothelia within the chorionic villous parenchyma (arrows); STB cell layer (arrowheads); intervillous space (asterisks). Bar, 50 &um;m.
From: Miranda J, Martín-Tapia D, Valdespino-Vázquez Y, Alarcón L, Espejel-Nuñez A, Guzmán-Huerta M, Muñoz-Medina JE, Shibayama M, Chávez-Munguía B, Estrada-Gutiérrez G, Lievano S, Ludert JE, González-Mariscal L.
Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.
Cells. 2019 Sep 29;8(10). pii: E1174.
doi: 10.3390/cells8101174.
This image is from an open access article distributed under the terms of the
Rat anti Mouse JAM-C
- Product Type
- Monoclonal Antibody
- Clone
- CRAM-18 F26
- Isotype
- IgG2a
- Specificity
- JAM-C
Antibody Quality and Performance Guarantee
| Rat anti Mouse JAM-c antibody, clone CRAM-18 F26 recognizes mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2. JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B. Rat anti Mouse JAM-c antibody, clone CRAM-18 F26 has been reported to inhibit transendothelial migration (Johnson-Léger et al. 2002). |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant soluble JAM-C.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Fischer rats were fused with cells of the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/25 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for JAM-C antibody
-
Aurrand-lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells.
J Biol Chem. 276 (4): 2733-41. -
Aurrand-lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members.
Blood. 98 (13): 3699-707. -
Johnson-léger, C.A. et al. (2002) Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.
Blood. 100 (7): 2479-86. -
Forsberg, E.C. et al. (2005) Differential expression of novel potential regulators in hematopoietic stem cells.
PLoS Genet. 1(3):e28. -
Radulovic, V. et al. (2019) Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis.
Cell Rep. 27 (10): 2826-2836.e5. -
Miranda, J. et al. (2019) Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.
Cells. 8 (10)Sep 29 [Epub ahead of print].
- Synonyms
- JAM-3
- UniProt
- Q9D8B7
- Q9BX67
- Entrez Gene
- JAM3
- Jam3
- GO Terms
- GO:0005886 plasma membrane
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0050900 leukocyte migration
- GO:0001780 neutrophil homeostasis
- GO:0002250 adaptive immune response
- GO:0002318 myeloid progenitor cell differentiation
- GO:0002523 leukocyte migration involved in inflammatory response
- GO:0005178 integrin binding
- View More GO Terms
- GO:0005923 tight junction
- GO:0030010 establishment of cell polarity
- GO:0007160 cell-matrix adhesion
- GO:0007286 spermatid development
- GO:0033010 paranodal junction
- GO:0042552 myelination
- GO:0042803 protein homodimerization activity
- GO:0046982 protein heterodimerization activity
- GO:0090138 regulation of actin cytoskeleton organization by cell-cell adhesion
View more products with JAM-C specificity
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