JAM-C antibody | CRAM-18 F26

Product Code Applications Pack Size List Price Your Price Qty
MCA5935T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 25 µg loader
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loader
MCA5935
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SDS Safety Datasheet SDS
C F IF 0.25 mg loader
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loader
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Rat anti Mouse JAM-c antibody, clone CRAM-18 F26 recognizes mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2.

JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.

Clone CRAM-18 F26 has been reported to inhibit transendothelial migration (Johnson-Léger et al. 2002).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant soluble JAM-C.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Fischer rats were fused with cells of the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/25
Immunofluorescence
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Rabbit F(ab')2 anti Rat IgG:FITC
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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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Description Rat IgG2a Negative Control

References for JAM-C antibody

  1. Aurrand-lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members.
    Blood. 98 (13): 3699-707.
  2. Johnson-léger, C.A. et al. (2002) Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.
    Blood. 100 (7): 2479-86.
  3. Aurrand-lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells.
    J Biol Chem. 276 (4): 2733-41.
  4. Forsberg, E.C. et al. (2005) Differential expression of novel potential regulators in hematopoietic stem cells.
    PLoS Genet. 1(3):e28.
  5. Miranda, J. et al. (2019) Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.
    Cells. 8 (10)Sep 29 [Epub ahead of print].
  6. Radulovic, V. et al. (2019) Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis.
    Cell Rep. 27 (10): 2826-2836.e5.

Flow Cytometry

Immunofluorescence

Synonyms
JAM-3
UniProt
Q9D8B7
Q9BX67
Entrez Gene
JAM3
Jam3
GO Terms
GO:0005886 plasma membrane
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0050900 leukocyte migration
GO:0001780 neutrophil homeostasis
GO:0002250 adaptive immune response
GO:0002318 myeloid progenitor cell differentiation
GO:0002523 leukocyte migration involved in inflammatory response
GO:0005178 integrin binding
GO:0005923 tight junction
GO:0030010 establishment of cell polarity
GO:0007160 cell-matrix adhesion
GO:0007286 spermatid development
GO:0033010 paranodal junction
GO:0042552 myelination
GO:0042803 protein homodimerization activity
GO:0046982 protein heterodimerization activity
GO:0090138 regulation of actin cytoskeleton organization by cell-cell adhesion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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