JAGGED2 antibody | HMJ2-1

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Hamster anti Mouse JAGGED2

Product Type
Monoclonal Antibody
Clone
HMJ2-1
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5708GA C F P 0.1 mg
MCA5708 C F P 0.25 mg
Hamster anti Mouse JAGGED2 antibody, clone HMJ2-1 recognizes Jagged2, one of the five major ligands of the Notch signaling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4.

The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression.

Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.

Jagged2 is expressed by stromal and thymic lymphoid cells, and by splenic macrophages and dendritic cells (DCs), and plays a vital role during limb, craniofacial, tooth, and thymic development, as well as being implicated in the maintenance and function of neuronal cells in both the central (CNS) and enteric (gastrointestinal) nervous system.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Jagged2-expressing CHO cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9QYE5 Related reagents
P97607 Related reagents
Q9Y219 Related reagents
Entrez Gene
Jag2 Related reagents
Jag2 Related reagents
JAG2 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0001666 response to hypoxia
GO:0001709 cell fate determination
GO:0005887 integral to plasma membrane
GO:0005112 Notch binding
GO:0007154 cell communication
GO:0007219 Notch signaling pathway
GO:0007283 spermatogenesis
GO:0007420 brain development
GO:0008083 growth factor activity
GO:0008584 male gonad development
GO:0009912 auditory receptor cell fate commitment
GO:0030326 embryonic limb morphogenesis
GO:0042127 regulation of cell proliferation
GO:0045061 thymic T cell selection
GO:0045595 regulation of cell differentiation
GO:0048471 perinuclear region of cytoplasm
GO:0001501 skeletal system development
GO:0001701 in utero embryonic development
GO:0003016 respiratory system process
GO:0005624 membrane fraction
GO:0007605 sensory perception of sound
GO:0042981 regulation of apoptosis
GO:0016331 morphogenesis of embryonic epithelium
GO:0030155 regulation of cell adhesion
GO:0042475 odontogenesis of dentine-containing tooth
GO:0042492 gamma-delta T cell differentiation
GO:0007049 cell cycle
GO:0007220 Notch receptor processing
GO:0030334 regulation of cell migration
Regulatory
For research purposes only

Applications of JAGGED2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Mouse spleen

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F

Application Based External Images

Flow Cytometry

Functional Assays

Product Specific References

References for JAGGED2 antibody

  1. Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.
    Int Immunol. 20 (6): 763-73.
  2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.
    Int Immunol. 21 (3): 295-301.
  3. Sekine, C. et al. (2012) Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.
    Arthritis Res Ther. 14: R45.

Further Reading

  1. Sander, G.R. et al. (2003) Expression of Notch1 and Jagged2 in the enteric nervous system.
    J Histochem Cytochem. 51 (7): 969-72.
  2. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
    Nat Rev Mol Cell Biol. 7 (9): 678-89.
  3. Iso, T. et al. (2003) Notch signaling in vascular development.
    Arterioscler Thromb Vasc Biol. 23 (4): 543-53.
  4. Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways.
    Immunity. 29: 691-703
  5. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.
    Immunol. Rev. 182: 215-227.