Gr-1 antibody | RB6-8C5














Rat anti Mouse Gr-1:Alexa Fluor® 488
Rat anti Mouse Gr-1:Alexa Fluor® 647
Rat anti Mouse Gr-1:FITC
Rat anti Mouse Gr-1:Pacific Blue®
Rat anti Mouse Gr-1
Rat anti Mouse Gr-1:RPE
Rat anti Mouse Gr-1:RPE-Alexa Fluor® 750
Rat anti Mouse Gr-1:Alexa Fluor® 700
Rat anti Mouse Gr-1:Amethyst Orange
- Product Type
- Monoclonal Antibody
- Clone
- RB6-8C5
- Isotype
- IgG2b
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2387A488T | F | 25 Tests/0.25ml |
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MCA2387A488 | F | 100 Tests/1ml |
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MCA2387A647T | F | 25 Tests/0.25ml |
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MCA2387A647 | F | 100 Tests/1ml |
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MCA2387F | F | 0.1 mg |
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MCA2387PB | F | 100 Tests/1ml |
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MCA2387T | C F IF IP WB | 25 µg |
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MCA2387GA | C F IF IP WB | 0.1 mg |
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MCA2387 | C F IF IP WB | 0.25 mg |
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MCA2387PE | F | 100 Tests |
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MCA2387P750 | F | 100 Tests |
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MCA2387A700 | F | 100 Tests/1ml |
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MCA2387AMO | F | 0.1 mg |
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The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 has been used successfully for the depletion of mature neutrophils in vivo (Czuprynski et al 1994, Daley et al. 2008).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor®488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to RPE-Alexa Fluor 750 - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor®700 - liquid
- Product Form
- Purified IgG conjugated to Amethyst Orange - liquid
- Reconstitution
- Reconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Carrier Free
- Yes
- Immunogen
- Normal murine bone marrow cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P35461
- Entrez Gene
- Ly6g
- GO Terms
- GO:0009897 external side of plasma membrane
- GO:0031225 anchored to membrane
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of Gr-1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
Flow Cytometry | 1/100 | 1/200 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Western Blotting | |||
Flow Cytometry | Neat | 1/5 | |
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
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Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2b Negative Control:FITC | MCA6006F | F | 0.1 mg |
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Rat IgG2b Negative Control:Pacific Blue® | MCA6006PB | F | 100 Tests/1ml |
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Rat IgG2b Negative Control:RPE-Alexa Fluor® 750 | MCA6006P750 | F | 100 Tests/1ml |
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Rat IgG2b Negative Control:Alexa Fluor® 700 | MCA6006A700 | F | 100 Tests/1ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Application Based External Images
Flow Cytometry
Immunofluorescence
Immunohistology - Paraffin
Western Blotting
Product Specific References
References for Gr-1 antibody
-
Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.
J Immunol. 151 (5): 2399-408. -
Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells.
J Immunol. 147 (1): 22-8. -
Czuprynski, C.J. et al. (1994) Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection.
J Immunol. 152 (4): 1836-46. -
Sumagin R et al. (2010) LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ.
J Immunol. 185 (11): 7057-66. -
Takano, K. et al. (2011) Successful treatment of acute lung injury with pitavastatin in septic mice: potential role of glucocorticoid receptor expression in alveolar macrophages.
J Pharmacol Exp Ther. 336: 381-90. -
Giroux, M. et al. (2011) SMAD3 prevents graft-versus-host disease by restraining Th1 differentiation and granulocyte-mediated tissue damage.
Blood.117: 1734-44. -
Suttmann, H. et al. (2006) Neutrophil granulocytes are required for effective Bacillus Calmette-Guérin immunotherapy of bladder cancer and orchestrate local immune responses.
Cancer Res. 66: 8250-7. -
Nix, R.N. et al. (2007) Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.
PLoS Pathog. 3: e193. -
Kanda, N. et al. (2011) Visfatin Enhances CXCL8, CXCL10, and CCL20 Production in Human Keratinocytes.
Endocrinology. 152: 3155-64. -
Conlan, J. and North, R. (1994) Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody.
J Exp Med. 179:259-68. -
Takebe, M. et al. (2014) Inhibition of histone deacetylases protects septic mice from lung and splenic apoptosis.
J Surg Res. 187 (2): 559-70. -
Francke, A. et al. (2011) Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties.
J Histochem Cytochem. 59: 813-25. -
Sharp, P.E. et al. (2013) FcγRIIb on myeloid cells and intrinsic renal cells rather than B cells protects from nephrotoxic nephritis.
J Immunol.190: 340-8. -
Hamers, A.A. et al. (2014) Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis.
Infect Immun. 82 (1): 253-64. -
Roche, J.A. et al. (2015) Myofiber damage precedes macrophage infiltration after in vivo injury in dysferlin-deficient a/j mouse skeletal muscle.
Am J Pathol. 185 (6): 1686-98. -
Lee, Y.S. et al. (2015) Interleukin-1 (IL-1) signaling in intestinal stromal cells controls KC/ CXCL1 secretion, which correlates with recruitment of IL-22- secreting neutrophils at early stages of Citrobacter rodentium infection.
Infect Immun. 83 (8): 3257-67. -
Heckelsmiller, K. et al. (2002) Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy.
Eur J Immunol. 32 (11): 3235-45. -
Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
J Clin Invest. 125 (11): 4281-94. -
Leblond, A.L. et al. (2015) Systemic and Cardiac Depletion of M2 Macrophage through CSF-1R Signaling Inhibition Alters Cardiac Function Post Myocardial Infarction.
PLoS One. 10 (9): e0137515. -
Kojo, K. et al. (2016) BLT1 signalling protects the liver against acetaminophen hepatotoxicity by preventing excessive accumulation of hepatic neutrophils.
Sci Rep. 6: 29650. -
Otsuru, S. et al. (2017) Hematopoietic derived cells do not contribute to osteogenesis as osteoblasts.
Bone. 94: 1-9. -
Wang, Y. et al. (2015) Proximal tubule-derived colony stimulating factor-1 mediates polarization of renal macrophages and dendritic cells, and recovery in acute kidney injury.
Kidney Int. 88 (6): 1274-1282. -
Cousins, F.L. et al. (2016) Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling.
Sci Rep. 6: 36748. -
Cotrina ML et al. (2017) Direct comparison of microglial dynamics and inflammatory profile in photothrombotic and arterial occlusion evoked stroke.
Neuroscience. 343: 483-94. -
Kamata, M. et al. (2019) Role of the high-affinity leukotriene B4 receptor signaling in fibrosis after unilateral ureteral obstruction in mice.
PLoS One. 14 (2): e0202842. -
Natanov, R. et al. (2018) Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass.
PLoS One. 13 (10): e0205437. -
Idowu, T.O. et al. (2020) Identification of specific Tie2 cleavage sites and therapeutic modulation in experimental sepsis.
Elife. 9: e59520.
Fluorescent Spectraviewer
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