CLEC2 antibody | 17D9

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Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 1

Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 2

Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 3

Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 4

Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 1 — Mouse peripheral blood platelets stained with FITC conjugated Rat anti Mouse CLEC-2 (**MCA5700F**)

Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 2 — Mouse peripheral blood platelets stained with phycoerythrin conjugated Rat anti Mouse CLEC-2 (**MCA5700PE**)

Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 3 — **Published customer image:**
FITC conjugated Rat anti Mouse CLEC2 antibody, clone 17D9 (**MCA5700F**) used to evaluate CLEC2 expression on platelets by flow cytometry
**Image caption:**
CLEC-2 is present at the surfaces of peripheral blood platelets, B cells and CD11b^high Gr-1^high cells at steady-state. Flow cytometry staining strategy to identify the haematopoietic cell subsets found in peripheral blood. Platelets and leucocytes were gated based on their small size (low FSC) and expression of CD41; or larger size (higher FSC) and absence of CD41, respectively. DAPI^Pos dead cells were excluded from the analysis. B and T lymphocytes (Lympho) and myeloid cells were gated based on their relative expression of CD3ε and CD19. Myeloid subsets were further separated based on their expression of CD11b and Gr-1. Cells positive for CLEC-2 are represented on histograms as a percentage of total cell counts in the population.
This analysis shows results from Clec1b^+/+ or Clec1b^-/- foetal liver reconstituted animals (WT FL or KO FL, respectively) challenged by intraperitoneal injection of 25μg LPS or PBS (Control). CLEC-2 expression was detected using the 17D9 antibody compared to its respective isotype control. This gating strategy was also applied to peripheral blood samples from tamoxifen-treated Clec1b^fl/flxRosa26^+/creERT2 and Clec1b^fl/fl mice and when analysing CLEC-2 expression with INU1.

From: Lowe KL, Navarro-Núñez L, Bénézech C, Nayar S, Kingston BL, Nieswandt B, Barone F, Watson SP, Buckley CD, Desanti GE.
The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 2015 Sep;45(9):2484-93.

Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 4 — **Published customer image:**
FITC conjugated Rat anti Mouse CLEC2 antibody, clone 17D9 (**MCA5700F**) used to evaluate CLEC2 expression on platelet and leukocyte subsets by flow cytometry
**Image caption:**
Flow cytometry staining strategy to identify the haematopoietic cell subsets found in the spleen. Platelets and leucocytes are gated based on their small size (low FSC) and expression of CD41; or larger size (higher FSC) and absence of CD41, respectively. Leucocyte populations are positively selected with CD45. Myeloid subsets are separated based on their expression of CD11b and Gr-1. B lymphocytes (B Lympho.) are defined as B220^pos CD11b^neg. Plasmacytoid dendritic cells (p-DC) are defined as B220^pos CD11c^pos Gr-1^pos. CD11c^pos B220^neg conventional dendritic cells (c-DC) are separated into two subsets based on their CD11b expression level. CLEC-2 expression is detected using the 17D9 antibody compared to its respective isotype control. Cells positive for CLEC-2 are represented on histograms as a percentage of total cell counts in the population.
This analysis shows results from Clec1b^+/+ or Clec1b^-/- foetal liver reconstituted animals (WT FL or KO FL, respectively) that were challenged by intraperitoneal injection with 25μg LPS or PBS only (Control). This gating strategy was also applied to analyse cells from the MLN.

From: Lowe KL, Navarro-Núñez L, Bénézech C, Nayar S, Kingston BL, Nieswandt B, Barone F, Watson SP, Buckley CD, Desanti GE.
The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 2015 Sep;45(9):2484-93.

Rat anti Mouse CLEC2:FITC

Rat anti Mouse CLEC2

Rat anti Mouse CLEC2:RPE

Product Type: Monoclonal Antibody
Clone: 17D9
Isotype: IgG2b

Product Code	Applications	Pack Size
MCA5700F	F	0.1 mg
MCA5700	F WB	0.25 mg
MCA5700PE	F	100 Tests

Rat anti Mouse CLEC2 antibody, clone 17D9 recognizes mouse C-type lectin domain family 1 member B, also known as CLEC2 or CLEC1B. CLEC2 acts as a receptor for rhodocytin, a platelet-aggregating protein found in snake venom. CLEC2 expression is not restricted to platelets and also acts as an activation receptor on neutrophils.

Product Details

Target Species: Mouse
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free: Yes
Immunogen: RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
Approx. Protein Concentrations: IgG concentration 0.1mg/ml
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Fusion Partners: Spleen cells from immunised Wistar rats were fused with cells of the rat Y3 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product is photosensitive and should be protected from light.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: Q9JL99 Related reagents
Entrez Gene: Clec1b Related reagents
GO Terms: GO:0004872 receptor activity; GO:0005887 integral to plasma membrane; GO:0005529 sugar binding; GO:0007166 cell surface receptor linked signaling pathway
Regulatory: For research purposes only

Applications of CLEC2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	1/25	1/200
Western Blotting
Flow Cytometry	Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)	STAR131A	1 ml	C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed)	STAR131B	0.5 mg	C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800	STAR16D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC	STAR17B	1 mg	F
Rabbit F(ab')2 anti Rat IgG:HRP	STAR21B	1 mg	C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)	STAR69	0.5 ml	F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)	STAR71D649GA	0.1 mg	F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)	STAR71D800GA	0.1 mg	F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	0.5 mg	C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)	STAR73	0.5 ml	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Mouse Seroblock FcR	BUF041A	0.1 mg	F
Mouse Seroblock FcR	BUF041B	0.5 mg	F
Mouse Seroblock FcR	BUF041A	0.1 mg	F
Mouse Seroblock FcR	BUF041B	0.5 mg	F

Product Specific References

References for CLEC2 antibody

Mourão-sá, D. et al. (2011) CLEC-2 signaling via Syk in myeloid cells can regulate inflammatory responses.
Eur J Immunol. 41 (10): 3040-53.
Mori, J. et al. (2012) Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B.
Arterioscler Thromb Vasc Biol. 32 (12): 2956-65.
Lowe, K.L. et al. (2015) The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 45 (9): 2484-93.
Nakamura-Ishizu A et al. (2015) CLEC-2 in megakaryocytes is critical for maintenance of hematopoietic stem cells in the bone marrow.
J Exp Med. 212(12):2133-46.
Meinders, M. et al. (2016) Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment.
PLoS One. 11 (5): e0154342.