CLEC2 antibody | 17D9
FITC conjugated Rat anti Mouse CLEC2 antibody, clone 17D9 (MCA5700F) used to evaluate CLEC2 expression on platelets by flow cytometry
Image caption:
CLEC-2 is present at the surfaces of peripheral blood platelets, B cells and CD11bhigh Gr-1high cells at steady-state. Flow cytometry staining strategy to identify the haematopoietic cell subsets found in peripheral blood. Platelets and leucocytes were gated based on their small size (low FSC) and expression of CD41; or larger size (higher FSC) and absence of CD41, respectively. DAPIPos dead cells were excluded from the analysis. B and T lymphocytes (Lympho) and myeloid cells were gated based on their relative expression of CD3ε and CD19. Myeloid subsets were further separated based on their expression of CD11b and Gr-1. Cells positive for CLEC-2 are represented on histograms as a percentage of total cell counts in the population.
This analysis shows results from Clec1b+/+ or Clec1b-/- foetal liver reconstituted animals (WT FL or KO FL, respectively) challenged by intraperitoneal injection of 25μg LPS or PBS (Control). CLEC-2 expression was detected using the 17D9 antibody compared to its respective isotype control. This gating strategy was also applied to peripheral blood samples from tamoxifen-treated Clec1bfl/flxRosa26+/creERT2 and Clec1bfl/fl mice and when analysing CLEC-2 expression with INU1.
From: Lowe KL, Navarro-Núñez L, Bénézech C, Nayar S, Kingston BL, Nieswandt B, Barone F, Watson SP, Buckley CD, Desanti GE.
The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 2015 Sep;45(9):2484-93.
FITC conjugated Rat anti Mouse CLEC2 antibody, clone 17D9 (MCA5700F) used to evaluate CLEC2 expression on platelet and leukocyte subsets by flow cytometry
Image caption:
Flow cytometry staining strategy to identify the haematopoietic cell subsets found in the spleen. Platelets and leucocytes are gated based on their small size (low FSC) and expression of CD41; or larger size (higher FSC) and absence of CD41, respectively. Leucocyte populations are positively selected with CD45. Myeloid subsets are separated based on their expression of CD11b and Gr-1. B lymphocytes (B Lympho.) are defined as B220pos CD11bneg. Plasmacytoid dendritic cells (p-DC) are defined as B220pos CD11cpos Gr-1pos. CD11cpos B220neg conventional dendritic cells (c-DC) are separated into two subsets based on their CD11b expression level. CLEC-2 expression is detected using the 17D9 antibody compared to its respective isotype control. Cells positive for CLEC-2 are represented on histograms as a percentage of total cell counts in the population.
This analysis shows results from Clec1b+/+ or Clec1b-/- foetal liver reconstituted animals (WT FL or KO FL, respectively) that were challenged by intraperitoneal injection with 25μg LPS or PBS only (Control). This gating strategy was also applied to analyse cells from the MLN.
From: Lowe KL, Navarro-Núñez L, Bénézech C, Nayar S, Kingston BL, Nieswandt B, Barone F, Watson SP, Buckley CD, Desanti GE.
The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 2015 Sep;45(9):2484-93.
Filter by Application:
F ResetRat anti Mouse CLEC2
- Product Type
- Monoclonal Antibody
- Clone
- 17D9
- Isotype
- IgG2b
- Specificity
- CLEC2
Antibody Quality and Performance Guarantee
| Rat anti Mouse CLEC2 antibody, clone 17D9 recognizes mouse C-type lectin domain family 1 member B, also known as CLEC2 or CLEC1B. CLEC2 acts as a receptor for rhodocytin, a platelet-aggregating protein found in snake venom. CLEC2 expression is not restricted to platelets and also acts as an activation receptor on neutrophils. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Wistar rats were fused with cells of the rat Y3 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CLEC2 antibody
-
Mourão-sá, D. et al. (2011) CLEC-2 signaling via Syk in myeloid cells can regulate inflammatory responses.
Eur J Immunol. 41 (10): 3040-53. -
Mori, J. et al. (2012) Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B.
Arterioscler Thromb Vasc Biol. 32 (12): 2956-65. -
Lowe, K.L. et al. (2015) The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 45 (9): 2484-93. -
Nakamura-Ishizu A et al. (2015) CLEC-2 in megakaryocytes is critical for maintenance of hematopoietic stem cells in the bone marrow.
J Exp Med. 212(12):2133-46. -
Meinders, M. et al. (2016) Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment.
PLoS One. 11 (5): e0154342. -
Hansen, M. et al. (2020) The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression.
Int J Mol Sci. 21 (3): 967. -
Kumode, T. et al. (2022) C-type lectin-like receptor 2 specifies a functionally distinct subpopulation within phenotypically defined hematopoietic stem cell population that contribute to emergent megakaryopoiesis.
Int J Hematol. Feb 01 [Epub ahead of print].
Further Reading
-
Kerrigan, A.M. et al. (2009) CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils.
J Immunol. 182 (7): 4150-7.
- Synonyms
- CLEC1B
- RRID
- AB_11152776
- UniProt
- Q9JL99
- Entrez Gene
- Clec1b
- GO Terms
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0007166 cell surface receptor linked signaling pathway
MCA5700
1602
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