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CLEC2 antibody | 17D9

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Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 1 Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 2 Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 3 Anti Mouse CLEC2 Antibody, clone 17D9 thumbnail image 4
Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 1
Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD31 (MCA2388A488) and Alexa Fluor 647 conjugated Rat IgG2b isotype control (MCA6006A647). Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD31 (MCA2388A488) and Alexa Fluor 647 conjugated Rat anti Mouse CLEC2 (MCA5700A647). All experiments performed on Mouse peripheral blood gated on live single cell platelets, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer
Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 2
Published customer image:
FITC conjugated Rat anti Mouse CLEC2 antibody, clone 17D9 (MCA5700F) used to evaluate CLEC2 expression on platelets by flow cytometry
Image caption:
CLEC-2 is present at the surfaces of peripheral blood platelets, B cells and CD11bhigh Gr-1high cells at steady-state. Flow cytometry staining strategy to identify the haematopoietic cell subsets found in peripheral blood. Platelets and leucocytes were gated based on their small size (low FSC) and expression of CD41; or larger size (higher FSC) and absence of CD41, respectively. DAPIPos dead cells were excluded from the analysis. B and T lymphocytes (Lympho) and myeloid cells were gated based on their relative expression of CD3ε and CD19. Myeloid subsets were further separated based on their expression of CD11b and Gr-1. Cells positive for CLEC-2 are represented on histograms as a percentage of total cell counts in the population.
This analysis shows results from Clec1b+/+ or Clec1b-/- foetal liver reconstituted animals (WT FL or KO FL, respectively) challenged by intraperitoneal injection of 25μg LPS or PBS (Control). CLEC-2 expression was detected using the 17D9 antibody compared to its respective isotype control. This gating strategy was also applied to peripheral blood samples from tamoxifen-treated Clec1bfl/flxRosa26+/creERT2 and Clec1bfl/fl mice and when analysing CLEC-2 expression with INU1.

From: Lowe KL, Navarro-Núñez L, Bénézech C, Nayar S, Kingston BL, Nieswandt B, Barone F, Watson SP, Buckley CD, Desanti GE.
The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 2015 Sep;45(9):2484-93.
Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 3
Published customer image:
FITC conjugated Rat anti Mouse CLEC2 antibody, clone 17D9 (MCA5700F) used to evaluate CLEC2 expression on platelet and leukocyte subsets by flow cytometry
Image caption:
Flow cytometry staining strategy to identify the haematopoietic cell subsets found in the spleen. Platelets and leucocytes are gated based on their small size (low FSC) and expression of CD41; or larger size (higher FSC) and absence of CD41, respectively. Leucocyte populations are positively selected with CD45. Myeloid subsets are separated based on their expression of CD11b and Gr-1. B lymphocytes (B Lympho.) are defined as B220pos CD11bneg. Plasmacytoid dendritic cells (p-DC) are defined as B220pos CD11cpos Gr-1pos. CD11cpos B220neg conventional dendritic cells (c-DC) are separated into two subsets based on their CD11b expression level. CLEC-2 expression is detected using the 17D9 antibody compared to its respective isotype control. Cells positive for CLEC-2 are represented on histograms as a percentage of total cell counts in the population.
This analysis shows results from Clec1b+/+ or Clec1b-/- foetal liver reconstituted animals (WT FL or KO FL, respectively) that were challenged by intraperitoneal injection with 25μg LPS or PBS only (Control). This gating strategy was also applied to analyse cells from the MLN.

From: Lowe KL, Navarro-Núñez L, Bénézech C, Nayar S, Kingston BL, Nieswandt B, Barone F, Watson SP, Buckley CD, Desanti GE.
The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 2015 Sep;45(9):2484-93.
Anti Mouse CLEC2 Antibody, clone 17D9 gallery image 4
Figure A. Alexa Fluor 647 conjugated Rat anti Mouse CD41 (MCA2245A647) and FITC conjugated Rat IgG2b isotype control (MCA6006F). Figure B . Alexa Fluor 647 conjugated Rat anti Mouse CD41 (MCA2245A647) and FITC conjugated Rat anti Mouse CLEC2 (MCA5700F). All experiments performed on murine peripheral blood gated on live single platelets, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer

Rat anti Mouse CLEC2:Alexa Fluor® 647

Rat anti Mouse CLEC2:FITC

Rat anti Mouse CLEC2

Rat anti Mouse CLEC2:RPE

Product Type
Monoclonal Antibody
Clone
17D9
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA5700A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA5700F
Datasheet
F
SDS
0.1 mg loader

MCA5700
Datasheet
F WB
SDS
0.25 mg loader

MCA5700PE
Datasheet
F
SDS
100 Tests loader

Rat anti Mouse CLEC2 antibody, clone 17D9 recognizes mouse C-type lectin domain family 1 member B, also known as CLEC2 or CLEC1B. CLEC2 acts as a receptor for rhodocytin, a platelet-aggregating protein found in snake venom. CLEC2 expression is not restricted to platelets and also acts as an activation receptor on neutrophils.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Wistar rats were fused with cells of the rat Y3 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product is photosensitive and should be protected from light.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q9JL99
Entrez Gene
Clec1b
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0007166 cell surface receptor linked signaling pathway
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CLEC2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry 1/25 1/200
Western Blotting
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Product Specific References

References for CLEC2 antibody

  1. Mourão-sá, D. et al. (2011) CLEC-2 signaling via Syk in myeloid cells can regulate inflammatory responses.
    Eur J Immunol. 41 (10): 3040-53.
  2. Mori, J. et al. (2012) Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B.
    Arterioscler Thromb Vasc Biol. 32 (12): 2956-65.
  3. Lowe, K.L. et al. (2015) The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
    Eur J Immunol. 45 (9): 2484-93.
  4. Nakamura-Ishizu A et al. (2015) CLEC-2 in megakaryocytes is critical for maintenance of hematopoietic stem cells in the bone marrow.
    J Exp Med. 212(12):2133-46.
  5. Meinders, M. et al. (2016) Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment.
    PLoS One. 11 (5): e0154342.

Further Reading

  1. Kerrigan, A.M. et al. (2009) CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils.
    J Immunol. 182 (7): 4150-7.

Fluorescent Spectraviewer

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