CLEC2 antibody | 17D9

Rat anti Mouse CLEC2

Product Type
Monoclonal Antibody
Clone
17D9
Isotype
IgG2b
Specificity
CLEC2

Product Code Applications Pack Size List Price Your Price Qty
MCA5700
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F WB 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CLEC2 antibody, clone 17D9 recognizes mouse C-type lectin domain family 1 member B, also known as CLEC2 or CLEC1B. CLEC2 acts as a receptor for rhodocytin, a platelet-aggregating protein found in snake venom. CLEC2 expression is not restricted to platelets and also acts as an activation receptor on neutrophils.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Wistar rats were fused with cells of the rat Y3 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CLEC2 antibody

  1. Mourão-sá, D. et al. (2011) CLEC-2 signaling via Syk in myeloid cells can regulate inflammatory responses.
    Eur J Immunol. 41 (10): 3040-53.
  2. Mori, J. et al. (2012) Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B.
    Arterioscler Thromb Vasc Biol. 32 (12): 2956-65.
  3. Lowe, K.L. et al. (2015) The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
    Eur J Immunol. 45 (9): 2484-93.
  4. Nakamura-Ishizu A et al. (2015) CLEC-2 in megakaryocytes is critical for maintenance of hematopoietic stem cells in the bone marrow.
    J Exp Med. 212(12):2133-46.
  5. Meinders, M. et al. (2016) Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment.
    PLoS One. 11 (5): e0154342.
  6. Hansen, M. et al. (2020) The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression.
    Int J Mol Sci. 21 (3): 967.
  7. Kumode, T. et al. (2022) C-type lectin-like receptor 2 specifies a functionally distinct subpopulation within phenotypically defined hematopoietic stem cell population that contribute to emergent megakaryopoiesis.
    Int J Hematol. Feb 01 [Epub ahead of print].

Further Reading

  1. Kerrigan, A.M. et al. (2009) CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils.
    J Immunol. 182 (7): 4150-7.

Synonyms
CLEC1B
RRID
AB_11152776
UniProt
Q9JL99
Entrez Gene
Clec1b
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0007166 cell surface receptor linked signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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