CLEC2 antibody | 17D9
Rat anti Mouse CLEC2
- Product Type
- Monoclonal Antibody
- Clone
- 17D9
- Isotype
- IgG2b
- Specificity
- CLEC2
Rat anti Mouse CLEC2 antibody, clone 17D9 recognizes mouse C-type lectin domain family 1 member B, also known as CLEC2 or CLEC1B. CLEC2 acts as a receptor for rhodocytin, a platelet-aggregating protein found in snake venom. CLEC2 expression is not restricted to platelets and also acts as an activation receptor on neutrophils. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Wistar rats were fused with cells of the rat Y3 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/25 | 1/200 | |
Western Blotting |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Source Reference
-
Mourão-sá, D. et al. (2011) CLEC-2 signaling via Syk in myeloid cells can regulate inflammatory responses.
Eur J Immunol. 41 (10): 3040-53.
References for CLEC2 antibody
-
Mori, J. et al. (2012) Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B.
Arterioscler Thromb Vasc Biol. 32 (12): 2956-65. -
Lowe, K.L. et al. (2015) The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.
Eur J Immunol. 45 (9): 2484-93. -
Nakamura-Ishizu A et al. (2015) CLEC-2 in megakaryocytes is critical for maintenance of hematopoietic stem cells in the bone marrow.
J Exp Med. 212(12):2133-46. -
Meinders, M. et al. (2016) Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment.
PLoS One. 11 (5): e0154342. -
Hansen, M. et al. (2020) The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression.
Int J Mol Sci. 21 (3): 967. -
Kumode, T. et al. (2022) C-type lectin-like receptor 2 specifies a functionally distinct subpopulation within phenotypically defined hematopoietic stem cell population that contribute to emergent megakaryopoiesis.
Int J Hematol. Feb 01 [Epub ahead of print].
Further Reading
-
Kerrigan, A.M. et al. (2009) CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils.
J Immunol. 182 (7): 4150-7.
- Synonyms
- CLEC1B
- RRID
- AB_11152776
- UniProt
- Q9JL99
- Entrez Gene
- Clec1b
- GO Terms
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0007166 cell surface receptor linked signaling pathway
MCA5700
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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