Rat anti Mouse THY-1.2 antibody, clone FF-10 recognizes the murine Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody.
Rat anti Mouse THY-1.2 antibody, clone FF-10 strongly induces homotypic adhesion of Thy1.2 positive cell lines.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- EL-4 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
Rho GTPase activator activity
negative regulation of protein kinase activity
negative regulation of cell migration
anchored to external side of plasma membrane
GPI anchor binding
positive regulation of GTPase activity
retinal cone cell development
focal adhesion assembly
negative regulation of axonogenesis
T cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
positive regulation of T cell activation
positive regulation of release of sequestered calcium ion into cytosol
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD90 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA1474 recognises a 30kD glycoprotein in immunoprecipitation and Western blotting assays.
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Secondary Antibodies Available
Product Specific References
References for CD90 antibody
Fan, X. et al. (1996) ICAM-1, -2, and LFA-1 independent homotypic T cell aggregation induced by a novel activating monoclonal antibody targeting the murine Thy-1 molecule.
Inflammation 20: 401.
Venugopalan P et al. (2016) Transplanted neurons integrate into adult retinas and respond to light.
Nat Commun. 7: 10472.
Szczerbal, I. et al. (2009) The spatial repositioning of adipogenesis genes is correlated with their expression status in a porcine mesenchymal stem cell adipogenesis model system.
Chromosoma. 118 (5): 647-63.
Catignas, K.K. et al. (2021) αV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo..
Glia. 69 (1): 91-108.
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