CD90 antibody | FF-10
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Rat anti Mouse THY-1.2
- Product Type
- Monoclonal Antibody
- Clone
- FF-10
- Isotype
- IgG2c
- Specificity
- CD90
Antibody Quality and Performance Guarantee
| Rat anti Mouse THY-1.2 antibody, clone FF-10 recognizes the murine Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody. Rat anti Mouse THY-1.2 antibody, clone FF-10 strongly induces homotypic adhesion of Thy1.2 positive cell lines. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- EL-4 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rat anti Mouse THY-1.2 antibody, clone FF-10 recognizes murine THY-1.2, also known as CD90. Thy-1.2 is a ~30 kDa glycoprotein as measured by immunoprecipitation and western blotting.
Source Reference
-
Fan, X. et al. (1996) ICAM-1, -2, and LFA-1 independent homotypic T cell aggregation induced by a novel activating monoclonal antibody targeting the murine Thy-1 molecule.
Inflammation 20: 401.
References for CD90 antibody
-
Venugopalan P et al. (2016) Transplanted neurons integrate into adult retinas and respond to light.
Nat Commun. 7: 10472. -
Szczerbal, I. et al. (2009) The spatial repositioning of adipogenesis genes is correlated with their expression status in a porcine mesenchymal stem cell adipogenesis model system.
Chromosoma. 118 (5): 647-63. -
Catignas, K.K. et al. (2021) αV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo..
Glia. 69 (1): 91-108. -
Blades, F. et al. (2020) Tyro3 Contributes to Retinal Ganglion Cell Function, Survival and Dendritic Density in the Mouse Retina.
Front Neurosci. 14: 840.
- Synonyms
- THY1
- RRID
- AB_322489
- UniProt
- P01831
- Entrez Gene
- Thy1
- GO Terms
- GO:0001525 angiogenesis
- GO:0007010 cytoskeleton organization
- GO:0005100 Rho GTPase activator activity
- GO:0006469 negative regulation of protein kinase activity
- GO:0016337 cell-cell adhesion
- GO:0030336 negative regulation of cell migration
- GO:0030425 dendrite
- GO:0030426 growth cone
- GO:0031362 anchored to external side of plasma membrane
- View More GO Terms
- GO:0034235 GPI anchor binding
- GO:0043547 positive regulation of GTPase activity
- GO:0045121 membrane raft
- GO:0046549 retinal cone cell development
- GO:0048041 focal adhesion assembly
- GO:0050771 negative regulation of axonogenesis
- GO:0050852 T cell receptor signaling pathway
- GO:0050860 negative regulation of T cell receptor signaling pathway
- GO:0050870 positive regulation of T cell activation
- GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
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