CD90 antibody | FF-10

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Rat anti Mouse THY-1.2

Product Type
Monoclonal Antibody
Clone
FF-10
Isotype
IgG2c
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1474 C F IP WB 0.25 mg
MCA1474T C F IP WB 25 µg
Rat anti Mouse THY-1.2 antibody, clone FF-10 recognizes the murine Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody.

Rat anti Mouse THY-1.2 antibody, clone FF-10 strongly induces homotypic adhesion of Thy1.2 positive cell lines.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
EL-4 cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01831 Related reagents
Entrez Gene
Thy1 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0005100 Rho GTPase activator activity
GO:0006469 negative regulation of protein kinase activity
GO:0016337 cell-cell adhesion
GO:0030336 negative regulation of cell migration
GO:0030425 dendrite
GO:0030426 growth cone
GO:0031362 anchored to external side of plasma membrane
GO:0034235 GPI anchor binding
GO:0043547 positive regulation of GTPase activity
GO:0045121 membrane raft
GO:0046549 retinal cone cell development
GO:0048041 focal adhesion assembly
GO:0050771 negative regulation of axonogenesis
GO:0050852 T cell receptor signaling pathway
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050870 positive regulation of T cell activation
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
Regulatory
For research purposes only

Applications of CD90 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA1474 recognises a 30kD glycoprotein in immunoprecipitation and Western blotting assays.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for CD90 antibody

  1. Fan, X. et al. (1996) ICAM-1, -2, and LFA-1 independent homotypic T cell aggregation induced by a novel activating monoclonal antibody targeting the murine Thy-1 molecule.
    Inflammation 20: 401.
  2. Venugopalan P et al. (2016) Transplanted neurons integrate into adult retinas and respond to light.
    Nat Commun. 7: 10472.
  3. Szczerbal, I. et al. (2009) The spatial repositioning of adipogenesis genes is correlated with their expression status in a porcine mesenchymal stem cell adipogenesis model system.
    Chromosoma. 118 (5): 647-63.