CD88 antibody | 20/70
Rat anti Mouse CD88 antibody, clone 20/70 (MCA2457GA) used for the evaluation of CD88 expression on immune cells by flow cytometry.
Image caption:
C5aR1 surface expression in innate and adaptive immune cells recruited into the lungs in response to OVA challenge.
(A) Histograms showing the surface expression levels of C5aR1 in lung eosinophils, and lung macrophages in PBS-treated or OVA-challenged GFP-C5aR1flox/flox animals using C5aR1-specific mAb 20/70. (B) Histograms showing the expression levels of GFP in CD11b+ cDCs and moDC of PBS-treated or OVA-challenged GFP-C5aR1flox/flox animals. The histograms are representative of three independent experiments.
From: Citation: Ender F, Wiese AV, Schmudde I, Sun J, Vollbrandt T, König P, et al. (2017)
Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.
PLoS ONE 12(2): e0172446.
Rat anti Mouse CD88 antibody, clone 20/70 (MCA2457GA) used for the evaluation of CD88 expression on immune cells by flow cytometry.
Image caption:
Surface expression of C5aR1.
Cells isolated from WT and C5aR1-/- mice under steady state conditions were stained for C5aR1 (CD88) as described in material and methods. (A/B) C5aR1 surface staining on eosinophils and tissue-associated alveolar macrophages (A) as well as different DC subsets (B) as described in Fig 2D. Data are representative of two independent cell isolations. Histograms show fluorescence intensity in cells from WT (solid line) and C5aR1-/- mice (dashed line).
From: Ender, F. et al. (2017)
Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.
PLoS ONE 12(2): e0172446.
Rat anti Mouse CD88 antibody, clone 20/70 (MCA2457GA) used for the identification of C5aR1 expressing cells in murine lung cryosections by immunohistochemistry.
Image caption:
Expression of C5aR1 and CD64 in lung tissue by IHC.
Precision cut lung slices (PCLS ) (300μm) from naïve C57BL/6 mice were generated and stained with anti CD64 and anti C5aR1 mABs. Stained slices were evaluated with confocal microscopy. Single-color and merged color display with CD64 (purple) and C5aR1(yellow). Data are representative of at least four independent experiments. AW: airway.
From: Hoffmann FM, Berger JL, Lingel I, Laumonnier Y, Lewkowich IP, Schmudde I, König P.
Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung.
Front Immunol. 2018 May 16;9:1046.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD88 antibody, clone 20/70 recognizes murine CD88, also known as C5a anaphylatoxin chemotactic receptor 1 (C5aR1), a member of the G-protein coupled receptor 1 family. CD88 is a ~45 kDa multi pass membrane protein and functions as a receptor for the complement component C5a, a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.
Rat anti Mouse CD88 antibody, clone 20/70 is able to block the binding of the C5a to murine CD88, (Soruri et al. 2003).
Rat anti Mouse CD88 antibody, clone 20/70 is able to block the binding of the C5a to murine CD88, (Soruri et al. 2003).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- RBL-2H3 transfected cells expressing murine C5aR.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised Lou/c were fused with cells of the X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P30993
- Entrez Gene
- C5ar1
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004944 C5a anaphylatoxin receptor activity
- GO:0050830 defense response to Gram-positive bacterium
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010759 positive regulation of macrophage chemotaxis
- GO:0030593 neutrophil chemotaxis
- GO:0032494 response to peptidoglycan
- GO:0045766 positive regulation of angiogenesis
- GO:0090023 positive regulation of neutrophil chemotaxis
- Regulatory
- For research purposes only
Applications of CD88 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Product Specific References
References for CD88 antibody
-
Soruri, A. et al. (2003) Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody.
Immunol Lett. 88 (1): 47-52. -
Godau, J. et al. (2004) C5a initiates the inflammatory cascade in immune complex peritonitis.
J Immunol. 173 (5): 3437-45. -
Baelder, R. et al. (2005) Pharmacological targeting of anaphylatoxin receptors during the effector phase of allergic asthma suppresses airway hyperresponsiveness and airway inflammation.
J Immunol. 174 (2): 783-9. -
Rittirsch, D. et al. (2008) Functional roles for C5a receptors in sepsis.
Nat Med. 14: 551-7. -
Shushakova, N. (2002) C5a anaphylatoxin is a major regulator of activating versus inhibitory FcgammaRs in immune complex-induced lung disease.
J Clin Invest. 110: 1823-30. -
Wang, R. et al. (2013) Disruption of the Complement Anaphylatoxin Receptor C5L2 Exacerbates Inflammation in Allergic Contact Dermatitis.
J Immunol. 191: 4001-9. -
Karsten, C.M. et al. (2015) Monitoring and cell-specific deletion of C5aR1 using a novel floxed GFP-C5aR1 reporter knock-in mouse.
J Immunol. 194 (4): 1841-55. -
Uenoyama, A. et al. (2016) Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
Biosci Biotechnol Biochem. 11: 1-12. -
Wang R et al. (2016) C5L2, the Second C5a Anaphylatoxin Receptor Suppresses Lipopolysaccharide-induced Acute Lung Injury.
Am J Respir Cell Mol Biol. Jun 10. [Epub ahead of print] -
Ender F et al. (2017) Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.
PLoS One. 12 (2): e0172446. -
Fusakio, M.E. et al. (2011) C5a regulates NKT and NK cell functions in sepsis.
J Immunol. 187 (11): 5805-12. -
Hoffmann, F.M. et al. (2018) Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung.
Front Immunol. 9: 1046.
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