CD88 antibody | 20/70

Rat anti Mouse CD88

Product Type
Monoclonal Antibody
Clone
20/70
Isotype
IgG2b
Specificity
CD88

Product Code Applications Pack Size List Price Your Price Qty
MCA2457GA
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SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
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Rat anti Mouse CD88 antibody, clone 20/70 recognizes murine CD88, also known as C5a anaphylatoxin chemotactic receptor 1 (C5aR1), a member of the G-protein coupled receptor 1 family. CD88 is a ~45 kDa multi pass membrane protein and functions as a receptor for the complement component C5a, a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.

Rat anti Mouse CD88 antibody, clone 20/70 is able to block the binding of the C5a to murine CD88, (Soruri et al. 2003).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
RBL-2H3 transfected cells expressing murine C5aR.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised Lou/c were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD88 antibody

  1. Soruri, A. et al. (2003) Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody.
    Immunol Lett. 88 (1): 47-52.
  2. Godau, J. et al. (2004) C5a initiates the inflammatory cascade in immune complex peritonitis.
    J Immunol. 173 (5): 3437-45.
  3. Baelder, R. et al. (2005) Pharmacological targeting of anaphylatoxin receptors during the effector phase of allergic asthma suppresses airway hyperresponsiveness and airway inflammation.
    J Immunol. 174 (2): 783-9.
  4. Rittirsch, D. et al. (2008) Functional roles for C5a receptors in sepsis.
    Nat Med. 14: 551-7.
  5. Shushakova, N. (2002) C5a anaphylatoxin is a major regulator of activating versus inhibitory FcgammaRs in immune complex-induced lung disease.
    J Clin Invest. 110: 1823-30.
  6. Wang, R. et al. (2013) Disruption of the Complement Anaphylatoxin Receptor C5L2 Exacerbates Inflammation in Allergic Contact Dermatitis.
    J Immunol. 191: 4001-9.
  7. Karsten, C.M. et al. (2015) Monitoring and cell-specific deletion of C5aR1 using a novel floxed GFP-C5aR1 reporter knock-in mouse.
    J Immunol. 194 (4): 1841-55.
  8. Uenoyama, A. et al. (2016) Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
    Biosci Biotechnol Biochem. 11: 1-12.
  9. View The Latest Product References
  10. Wang R et al. (2016) C5L2, the Second C5a Anaphylatoxin Receptor Suppresses Lipopolysaccharide-induced Acute Lung Injury.
    Am J Respir Cell Mol Biol. Jun 10. [Epub ahead of print]
  11. Ender F et al. (2017) Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.
    PLoS One. 12 (2): e0172446.
  12. Fusakio, M.E. et al. (2011) C5a regulates NKT and NK cell functions in sepsis.
    J Immunol. 187 (11): 5805-12.
  13. Hoffmann, F.M. et al. (2018) Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung.
    Front Immunol. 9: 1046.

Flow Cytometry

Functional Assays

Synonyms
C5aR
RRID
AB_770088
UniProt
P30993
Entrez Gene
C5ar1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004944 C5a anaphylatoxin receptor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010759 positive regulation of macrophage chemotaxis
GO:0030593 neutrophil chemotaxis
GO:0032494 response to peptidoglycan
GO:0045766 positive regulation of angiogenesis
GO:0090023 positive regulation of neutrophil chemotaxis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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