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CD88 antibody | 20/70

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Anti Mouse CD88 Antibody, clone 20/70 thumbnail image 1 Anti Mouse CD88 Antibody, clone 20/70 thumbnail image 2 Anti Mouse CD88 Antibody, clone 20/70 thumbnail image 3
Anti Mouse CD88 Antibody, clone 20/70 gallery image 1
Published customer image:
Rat anti Mouse CD88 antibody, clone 20/70 (MCA2457GA) used for the evaluation of CD88 expression on immune cells by flow cytometry.
Image caption:
C5aR1 surface expression in innate and adaptive immune cells recruited into the lungs in response to OVA challenge.
(A) Histograms showing the surface expression levels of C5aR1 in lung eosinophils, and lung macrophages in PBS-treated or OVA-challenged GFP-C5aR1flox/flox animals using C5aR1-specific mAb 20/70. (B) Histograms showing the expression levels of GFP in CD11b+ cDCs and moDC of PBS-treated or OVA-challenged GFP-C5aR1flox/flox animals. The histograms are representative of three independent experiments.

From: Citation: Ender F, Wiese AV, Schmudde I, Sun J, Vollbrandt T, König P, et al. (2017)
Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.
PLoS ONE 12(2): e0172446.
Anti Mouse CD88 Antibody, clone 20/70 gallery image 2
Published customer image:
Rat anti Mouse CD88 antibody, clone 20/70 (MCA2457GA) used for the evaluation of CD88 expression on immune cells by flow cytometry.
Image caption:
Surface expression of C5aR1.
Cells isolated from WT and C5aR1-/- mice under steady state conditions were stained for C5aR1 (CD88) as described in material and methods. (A/B) C5aR1 surface staining on eosinophils and tissue-associated alveolar macrophages (A) as well as different DC subsets (B) as described in Fig 2D. Data are representative of two independent cell isolations. Histograms show fluorescence intensity in cells from WT (solid line) and C5aR1-/- mice (dashed line).
From: Ender, F. et al. (2017)
Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.
PLoS ONE 12(2): e0172446.
Anti Mouse CD88 Antibody, clone 20/70 gallery image 3
Published customer image:
Rat anti Mouse CD88 antibody, clone 20/70 (MCA2457GA) used for the identification of C5aR1 expressing cells in murine lung cryosections by immunohistochemistry.
Image caption:
Expression of C5aR1 and CD64 in lung tissue by IHC.
Precision cut lung slices (PCLS ) (300μm) from naïve C57BL/6 mice were generated and stained with anti CD64 and anti C5aR1 mABs. Stained slices were evaluated with confocal microscopy. Single-color and merged color display with CD64 (purple) and C5aR1(yellow). Data are representative of at least four independent experiments. AW: airway.

From: Hoffmann FM, Berger JL, Lingel I, Laumonnier Y, Lewkowich IP, Schmudde I, König P.
Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung.
Front Immunol. 2018 May 16;9:1046.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rat anti Mouse CD88

Rat anti Mouse CD88:RPE

Product Type
Monoclonal Antibody
Clone
20/70
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA2457GA
Datasheet
F
SDS
0.1 mg loader

MCA2457PE
Datasheet
F
SDS
100 Tests loader

Rat anti Mouse CD88 antibody, clone 20/70 recognizes murine CD88, also known as C5a anaphylatoxin chemotactic receptor 1 (C5aR1), a member of the G-protein coupled receptor 1 family. CD88 is a ~45 kDa multi pass membrane protein and functions as a receptor for the complement component C5a, a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.

Rat anti Mouse CD88 antibody, clone 20/70 is able to block the binding of the C5a to murine CD88, (Soruri et al. 2003).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
RBL-2H3 transfected cells expressing murine C5aR.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised Lou/c were fused with cells of the X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P30993
Entrez Gene
C5ar1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004944 C5a anaphylatoxin receptor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010759 positive regulation of macrophage chemotaxis
GO:0030593 neutrophil chemotaxis
GO:0032494 response to peptidoglycan
GO:0045766 positive regulation of angiogenesis
GO:0090023 positive regulation of neutrophil chemotaxis
Regulatory
For research purposes only

Applications of CD88 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

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Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Application Based External Images

Flow Cytometry

Functional Assays

Product Specific References

References for CD88 antibody

  1. Soruri, A. et al. (2003) Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody.
    Immunol Lett. 88 (1): 47-52.
  2. Godau, J. et al. (2004) C5a initiates the inflammatory cascade in immune complex peritonitis.
    J Immunol. 173 (5): 3437-45.
  3. Baelder, R. et al. (2005) Pharmacological targeting of anaphylatoxin receptors during the effector phase of allergic asthma suppresses airway hyperresponsiveness and airway inflammation.
    J Immunol. 174 (2): 783-9.
  4. Rittirsch, D. et al. (2008) Functional roles for C5a receptors in sepsis.
    Nat Med. 14: 551-7.
  5. Shushakova, N. (2002) C5a anaphylatoxin is a major regulator of activating versus inhibitory FcgammaRs in immune complex-induced lung disease.
    J Clin Invest. 110: 1823-30.
  6. Wang, R. et al. (2013) Disruption of the Complement Anaphylatoxin Receptor C5L2 Exacerbates Inflammation in Allergic Contact Dermatitis.
    J Immunol. 191: 4001-9.
  7. Karsten, C.M. et al. (2015) Monitoring and cell-specific deletion of C5aR1 using a novel floxed GFP-C5aR1 reporter knock-in mouse.
    J Immunol. 194 (4): 1841-55.
  8. Uenoyama, A. et al. (2016) Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
    Biosci Biotechnol Biochem. 11: 1-12.
  9. Wang R et al. (2016) C5L2, the Second C5a Anaphylatoxin Receptor Suppresses Lipopolysaccharide-induced Acute Lung Injury.
    Am J Respir Cell Mol Biol. Jun 10. [Epub ahead of print]
  10. Ender F et al. (2017) Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.
    PLoS One. 12 (2): e0172446.
  11. Fusakio, M.E. et al. (2011) C5a regulates NKT and NK cell functions in sepsis.
    J Immunol. 187 (11): 5805-12.
  12. Hoffmann, F.M. et al. (2018) Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung.
    Front Immunol. 9: 1046.
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