CD88 antibody | 20/70



Rat anti Mouse CD88 antibody, clone 20/70 is able to block the binding of the C5a to murine CD88, (Soruri et al. 2003).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- RBL-2H3 transfected cells expressing murine C5aR.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised Lou/c were fused with cells of the X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P30993
- Entrez Gene
- C5ar1
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004944 C5a anaphylatoxin receptor activity
- GO:0050830 defense response to Gram-positive bacterium
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010759 positive regulation of macrophage chemotaxis
- GO:0030593 neutrophil chemotaxis
- GO:0032494 response to peptidoglycan
- GO:0045766 positive regulation of angiogenesis
- GO:0090023 positive regulation of neutrophil chemotaxis
- Regulatory
- For research purposes only
Applications of CD88 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/100 | |
Immunohistology - Frozen | |||
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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|
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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|
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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|
Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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|
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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|
Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Product Specific References
References for CD88 antibody
-
Soruri, A. et al. (2003) Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody.
Immunol Lett. 88 (1): 47-52. -
Godau, J. et al. (2004) C5a initiates the inflammatory cascade in immune complex peritonitis.
J Immunol. 173 (5): 3437-45. -
Baelder, R. et al. (2005) Pharmacological targeting of anaphylatoxin receptors during the effector phase of allergic asthma suppresses airway hyperresponsiveness and airway inflammation.
J Immunol. 174 (2): 783-9. -
Rittirsch, D. et al. (2008) Functional roles for C5a receptors in sepsis.
Nat Med. 14: 551-7. -
Shushakova, N. (2002) C5a anaphylatoxin is a major regulator of activating versus inhibitory FcgammaRs in immune complex-induced lung disease.
J Clin Invest. 110: 1823-30. -
Wang, R. et al. (2013) Disruption of the Complement Anaphylatoxin Receptor C5L2 Exacerbates Inflammation in Allergic Contact Dermatitis.
J Immunol. 191: 4001-9. -
Karsten, C.M. et al. (2015) Monitoring and cell-specific deletion of C5aR1 using a novel floxed GFP-C5aR1 reporter knock-in mouse.
J Immunol. 194 (4): 1841-55. -
Uenoyama, A. et al. (2016) Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
Biosci Biotechnol Biochem. 11: 1-12. -
Wang R et al. (2016) C5L2, the Second C5a Anaphylatoxin Receptor Suppresses Lipopolysaccharide-induced Acute Lung Injury.
Am J Respir Cell Mol Biol. Jun 10. [Epub ahead of print] -
Ender F et al. (2017) Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.
PLoS One. 12 (2): e0172446. -
Fusakio, M.E. et al. (2011) C5a regulates NKT and NK cell functions in sepsis.
J Immunol. 187 (11): 5805-12. -
Hoffmann, F.M. et al. (2018) Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung.
Front Immunol. 9: 1046.
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