CD88 antibody | 10/92

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MCA2456GA
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C F IF 0.1 mg loader
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MCA2456
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C F IF 0.25 mg loader
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Rat anti Mouse CD88 antibody, clone 10/92 recognizes murine CD88, a 45 kDa G-protein coupled cell surface receptor, otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, a potent proinflammatory molecule and chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.

Rat anti Mouse CD88 antibody, clone 10/92 does not block the binding of the C5a to murine CD88 (Souri et al. 2003).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
RBL-2H3 transfected cells expressing murine C5aR.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised Lou/c rats were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Functional Assays
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Method sheets available on request.

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References for CD88 antibody

  1. Soruri, A. et al. (2003) Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody.
    Immunol Lett. 88:47-52.
  2. Shagdarsuren, E. et al. (2010) C5a Receptor Targeting in Neointima Formation After Arterial Injury in Atherosclerosis-Prone Mice.
    Circulation. 122: 1026-36.
  3. Manthey, H.D. et al. (2011) Complement C5a inhibition reduces atherosclerosis in ApoE-/- mice.
    FASEB J. 25: 2447-55.
  4. Cudaback, E. et al. (2011) Apolipoprotein E isoform-dependent microglia migration.
    FASEB J. 25: 2082-91.
  5. Ager, R.R. et al. (2010) Microglial C5aR (CD88) expression correlates with amyloid-beta deposition in murine models of Alzheimer's disease.
    J Neurochem. 113: 389-401
  6. Brennan, F.H. et al. (2015) The Complement Receptor C5aR Controls Acute Inflammation and Astrogliosis following Spinal Cord Injury.
    J Neurosci. 35 (16): 6517-31.
  7. Li, R. et al. (2013) C5L2: a controversial receptor of complement anaphylatoxin, C5a.
    FASEB J. 27 (3): 855-64.
  8. Denny, K.J. et al. (2013) C5a receptor signaling prevents folate deficiency-induced neural tube defects in mice.
    J Immunol. 190 (7): 3493-9.
  9. View The Latest Product References
  10. Pavlovski, D. et al. (2012) Generation of complement component C5a by ischemic neurons promotes neuronal apoptosis.
    FASEB J. 26 (9): 3680-90.
  11. Benson, M.J. et al. (2017) The effects of C5aR1 on leukocyte infiltration following pilocarpine-induced status epilepticus.
    Epilepsia. 58 (4): e54-e58.
  12. Ramstead, A.G. et al. (2016) Roles of Toll-Like Receptor 2 (TLR2), TLR4, and MyD88 during Pulmonary Coxiella burnetii Infection.
    Infect Immun. 84 (4): 940-9.
  13. Benson, M.J. et al. (2015) A novel anticonvulsant mechanism via inhibition of complement receptor C5ar1 in murine epilepsy models.
    Neurobiol Dis. 76: 87-97.
  14. Hernandez, M.X. et al. (2017) C5a Increases the Injury to Primary Neurons Elicited by Fibrillar Amyloid Beta.
    ASN Neuro. 9 (1): 1759091416687871.

Immunofluorescence

Synonyms
C5aR
RRID
AB_770085
UniProt
P30993
Entrez Gene
C5ar1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004944 C5a anaphylatoxin receptor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010759 positive regulation of macrophage chemotaxis
GO:0030593 neutrophil chemotaxis
GO:0032494 response to peptidoglycan
GO:0045766 positive regulation of angiogenesis
GO:0090023 positive regulation of neutrophil chemotaxis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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