CD88 antibody | 10/92
Rat anti Mouse CD88
- Product Type
- Monoclonal Antibody
- Clone
- 10/92
- Isotype
- IgG2a
- Specificity
- CD88
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD88 antibody, clone 10/92 recognizes murine CD88, a 45 kDa G-protein coupled cell surface receptor, otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, a potent proinflammatory molecule and chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes. Rat anti Mouse CD88 antibody, clone 10/92 does not block the binding of the C5a to murine CD88 (Souri et al. 2003). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- RBL-2H3 transfected cells expressing murine C5aR.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised Lou/c rats were fused with cells of the X63-Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Functional Assays | |||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Method sheets available on request.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for CD88 antibody
-
Soruri, A. et al. (2003) Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody.
Immunol Lett. 88:47-52. -
Ager, R.R. et al. (2010) Microglial C5aR (CD88) expression correlates with amyloid-beta deposition in murine models of Alzheimer's disease.
J Neurochem. 113: 389-401 -
Shagdarsuren, E. et al. (2010) C5a Receptor Targeting in Neointima Formation After Arterial Injury in Atherosclerosis-Prone Mice.
Circulation. 122: 1026-36. -
Cudaback, E. et al. (2011) Apolipoprotein E isoform-dependent microglia migration.
FASEB J. 25: 2082-91. -
Manthey, H.D. et al. (2011) Complement C5a inhibition reduces atherosclerosis in ApoE-/- mice.
FASEB J. 25: 2447-55. -
Pavlovski, D. et al. (2012) Generation of complement component C5a by ischemic neurons promotes neuronal apoptosis.
FASEB J. 26 (9): 3680-90. -
Denny, K.J. et al. (2013) C5a receptor signaling prevents folate deficiency-induced neural tube defects in mice.
J Immunol. 190 (7): 3493-9. -
Li, R. et al. (2013) C5L2: a controversial receptor of complement anaphylatoxin, C5a.
FASEB J. 27 (3): 855-64.
View The Latest Product References
-
Benson, M.J. et al. (2015) A novel anticonvulsant mechanism via inhibition of complement receptor C5ar1 in murine epilepsy models.
Neurobiol Dis. 76: 87-97. -
Brennan, F.H. et al. (2015) The Complement Receptor C5aR Controls Acute Inflammation and Astrogliosis following Spinal Cord Injury.
J Neurosci. 35 (16): 6517-31. -
Hernandez, M.X. et al. (2017) C5a Increases the Injury to Primary Neurons Elicited by Fibrillar Amyloid Beta.
ASN Neuro. 9 (1): 1759091416687871. -
Benson, M.J. et al. (2017) The effects of C5aR1 on leukocyte infiltration following pilocarpine-induced status epilepticus.
Epilepsia. 58 (4): e54-e58. -
Cao, D. et al. (2022) Vascular Endothelial Cells Produce Coagulation Factors That Control Their Growth via Joint Protease-Activated Receptor and C5a Receptor 1 (CD88) Signaling.
Am J Pathol. 192 (2): 361-378.
- Synonyms
- C5aR
- RRID
- AB_770085
- UniProt
- P30993
- Entrez Gene
- C5ar1
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004944 C5a anaphylatoxin receptor activity
- GO:0050830 defense response to Gram-positive bacterium
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010759 positive regulation of macrophage chemotaxis
- GO:0030593 neutrophil chemotaxis
- GO:0032494 response to peptidoglycan
- GO:0045766 positive regulation of angiogenesis
- View More GO Terms
- GO:0090023 positive regulation of neutrophil chemotaxis
View more products with CD88 specificity
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