CD86 antibody | GL-1
Rat anti mouse CD86 antibody, clone GL-1 (MCA6076GA) used to identify CD86 expressing cells in murine tumor tissue by immunofluorescence.
Image caption:
Reduction of M2-like TAMs by melittin–dKLA in a mouse model of melanoma. Single cells were harvested from tumor tissues. (D) Expression of CD86 and CD206 in tumor tissues was determined by immunofluorescence staining assay. Confocal images of a tumor tissue slice stained for CD86 (green) and nucleus (DAPI; blue). (E) The graph is presented as intensity relative to PBS.All data are presented as means ± SD (n = 5); * p <0.05 versus the PBS group.
From: Han, I.-H.; Jeong, C.; Yang, J.; Park, S.-H.; Hwang, D.-S.; Bae, H.
Therapeutic Effect of Melittin–dKLA Targeting Tumor-Associated Macrophages in Melanoma.
Int. J. Mol. Sci. 2022, 23, 3094.
doi: 10.3390/ijms23063094
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IF ResetRat anti Mouse CD86
- Product Type
- Monoclonal Antibody
- Clone
- GL-1
- Isotype
- IgG2a
- Specificity
- CD86
Antibody Quality and Performance Guarantee
| Rat anti mouse CD86 antibody, clone GL-1 recognizes mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, and a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T lymphocytes. Expression of CD86 on these cell populations can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T lymphocyte stimulation. Interaction of CD86 with CD28 promotes a number of T lymphocyte activities, whereas the binding of CD86 to CTLA4 inhibits T lymphocyte responses. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- LPS-activated CBA/Ca mouse splenic B lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
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Log in | ||||||
| Description | Rat IgG2a Negative Control | ||||||
References for CD86 antibody
-
Pechhold, K. et al. (1997) Inflammatory cytokines IFN-gamma plus TNF-alpha induce regulated expression of CD80 (B7-1) but not CD86 (B7-2) on murine fibroblasts.
J Immunol. 158 (10): 4921-9. -
Han, I. et al. (2022) Therapeutic Effect of Melittin–dKLA Targeting Tumor-Associated Macrophages in Melanoma
Int J Med Sci. 23 (6): 3094.
- Synonyms
- B7-2
- UniProt
- P42082
- Entrez Gene
- Cd86
- GO Terms
- GO:0002224 toll-like receptor signaling pathway
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0009897 external side of plasma membrane
- GO:0042113 B cell activation
- GO:0043231 intracellular membrane-bounded organelle
MCA6076GA
165139
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