CD86 antibody | GL-1

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Rat anti Mouse CD86:FITC

Rat anti Mouse CD86

Rat anti Mouse CD86:RPE

Product Type
Monoclonal Antibody
Clone
GL-1
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6076F F 0.1 mg
MCA6076GA F 0.1 mg
MCA6076PE F 0.1 mg
Rat anti mouse CD86 antibody, clone GL-1 recognizes mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, and a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T lymphocytes. Expression of CD86 on these cell populations can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T lymphocyte stimulation. Interaction of CD86 with CD28 promotes a number of T lymphocyte activities, whereas the binding of CD86 to CTLA4 inhibits T lymphocyte responses.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
LPS-activated CBA/Ca mouse splenic B lymphocytes
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch

More Information

UniProt
P42082 Related reagents
Entrez Gene
Cd86 Related reagents
GO Terms
GO:0002224 toll-like receptor signaling pathway
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009897 external side of plasma membrane
GO:0042113 B cell activation
GO:0043231 intracellular membrane-bounded organelle
Regulatory
For research purposes only

Applications of CD86 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5
Flow Cytometry 1/5
Flow Cytometry Neat 1/2
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F 100 Tests F
Rat IgG2a Negative Control MCA1212 1 ml E F
Rat IgG2a Negative Control:RPE MCA1212PE 100 Tests F

Product Specific References

References for CD86 antibody

  1. Pechhold, K. et al. (1997) Inflammatory cytokines IFN-gamma plus TNF-alpha induce regulated expression of CD80 (B7-1) but not CD86 (B7-2) on murine fibroblasts.
    J Immunol. 158 (10): 4921-9.