CD86 antibody | GL-1
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|Rat anti mouse CD86 antibody, clone GL-1 recognizes mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, and a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T lymphocytes. Expression of CD86 on these cell populations can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T lymphocyte stimulation. Interaction of CD86 with CD28 promotes a number of T lymphocyte activities, whereas the binding of CD86 to CTLA4 inhibits T lymphocyte responses.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- LPS-activated CBA/Ca mouse splenic B lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- For research purposes only
- 12 months from date of despatch
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
References for CD86 antibody
Pechhold, K. et al. (1997) Inflammatory cytokines IFN-gamma plus TNF-alpha induce regulated expression of CD80 (B7-1) but not CD86 (B7-2) on murine fibroblasts.
J Immunol. 158 (10): 4921-9.
Han, I. et al. (2022) Therapeutic Effect of Melittin–dKLA Targeting Tumor-Associated Macrophages in Melanoma
Int J Med Sci. 23 (6): 3094.
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