CD83 antibody | 3D11

Rat anti Mouse CD83

Product Type
Monoclonal Antibody
Clone
3D11
Isotype
IgG2a
Specificity
CD83

Product Code Applications Pack Size List Price Your Price Qty
MCA2747GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD83 antibody, clone 3D11 recognizes murine CD83, a 45 kDa type 1 glyoprotein belonging to the immunoglobulin superfamily. Originally described as a maturation marker for dendritic cells, CD83 plays a role in the regulation of antigen presentation, thymic T-cell maturation, B-cell function and peripheral T-cell activation. Activated dendritic cells and B-cells release a soluble form of CD83 that is reported to have an immunosuppressive effect, suggesting a therapeutic role for sCD83 in the regulation of immune disorders.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Rabbit F(ab')2 anti Rat IgG:FITC
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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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Description Rat IgG2a Negative Control

References for CD83 antibody

  1. Fujimoto, Y. & Tedder, T.F. (2006) CD83: a regulatory molecule of the immune system with great potential for therapeutic application.
    J Med Dent Sci. 53 (2): 85-91.
  2. He, Y.T. et al. (2016) In vitro generation of cytotoxic T lymphocyte response using dendritic cell immunotherapy in osteosarcoma.
    Oncol Lett. 12 (2): 1101-6.
  3. El-Ashmawy, N.E. et al. (2015) In vitro and in vivo studies of the immunomodulatory effect of Echinacea purpurea on dendritic cells.
    Journal of Genetic Engineering and Biotechnology. 13 (2): 185-92.

Synonyms
HB15
RRID
AB_10614522
UniProt
O88324
Entrez Gene
Cd83
GO Terms
GO:0016021 integral to membrane
GO:0009897 external side of plasma membrane
GO:0014070 response to organic cyclic compound
GO:0032713 negative regulation of interleukin-4 production
GO:0032733 positive regulation of interleukin-10 production
GO:0032743 positive regulation of interleukin-2 production
GO:0043372 positive regulation of CD4-positive, alpha beta T cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products