CD8 Alpha antibody | YTS105.18

Rat anti Mouse CD8

Product Type
Monoclonal Antibody
Clone
YTS105.18
Isotype
IgG2a
Specificity
CD8 Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA1108GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 25 µg loader
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loader
MCA1108G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD8, clone YTS105.18 recognizes a non polymorphic epitope on the mouse CD8 alpha chain. This antibody has been reported to block MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies (Cobbold et al. 1990 & Wise et al. 1998).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Mouse spleen cells.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Rat IgG2a Negative Control

References for CD8 Alpha antibody

  1. Qin, S.X. et al. (1990) Induction of tolerance in peripheral T cells with monoclonal antibodies.
    Eur J Immunol. 20 (12): 2737-45.
  2. Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
    Eur J Immunol. 20 (12): 2747-55.
  3. Wise, M.P. et al. (1998) Linked suppression of skin graft rejection can operate through indirect recognition.
    J Immunol. 161 (11): 5813-6.
  4. Lacroix-Lamande, S. et al. (2009) Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
    PLoS One. 4: 1-8.
  5. Auray, G. et al. (2007) Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection
    Microbes Infect. 9: 574-82.
  6. Sroga, J.M. et al. (2003) Rats and mice exhibit distinct inflammatory reactions after spinal cord injury.
    J Comp Neurol. 462: 223-40.
  7. Karlsson, M.R. et al. (2010) Hypersensitivity and oral tolerance in the absence of a secretory immune system.
    Allergy. 65: 561-70.
  8. Himoudi, N. et al. (2007) Development of anti-PAX3 immune responses; a target for cancer immunotherapy
    Cancer Immunol Immunother. 56: 1381-95.
  9. View The Latest Product References
  10. Nakashima, H. et al. (2011) A Novel Combination Immunotherapy for Cancer by IL-13Rα2-Targeted DNA Vaccine and Immunotoxin in Murine Tumor Models.
    J Immunol. 187: 4935-46.
  11. Shaw, T.N. et al. (2015) Perivascular Arrest of CD8+ T Cells Is a Signature of Experimental Cerebral Malaria.
    PLoS Pathog. 11 (11): e1005210.
  12. Jalili, R.B. et al. (2018) Fibroblast cell-based therapy prevents induction of alopecia areata in an experimental model.
    Cell Transplant. 27 (6): 994-1004.
  13. Zhao, Q. et al. (2019) Tumor-targeted IL-12 combined with tumor resection yields a survival-favorable immune profile.
    J Immunother Cancer. 7 (1): 154.
  14. Mohanta, S.K. et al. (2022) Neuroimmune cardiovascular interfaces control atherosclerosis.
    Nature. Apr 27 [Epub ahead of print].

Flow Cytometry

Synonyms
LY-2
RRID
AB_322819
UniProt
P01731
Entrez Gene
Cd8a
GO Terms
GO:0002456 T cell mediated immunity
GO:0016021 integral to membrane
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0042803 protein homodimerization activity
GO:0045065 cytotoxic T cell differentiation
GO:0050850 positive regulation of calcium-mediated signaling
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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