CD71 antibody | YTA74.4

Rat anti Mouse CD71

Product Type
Monoclonal Antibody
Clone
YTA74.4
Isotype
IgG2a
Specificity
CD71

Product Code Applications Pack Size List Price Your Price Qty
MCA1033G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.25 mg loader
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loader
MCA1033GA
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SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
MCA1033GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 25 µg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD71 antibody, clone YTA74.4 recognizes the mouse Transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron.

Rat anti Mouse CD71 antibody, clone YTA74.4 blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (Trowbridge et al. 1982).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Concanavilin A activated mouse spleen cells.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.

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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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Description Rat IgG2a Negative Control

References for CD71 antibody

  1. Millot, S. et al. (2010) Erythropoietin stimulates spleen BMP4-dependent stress erythropoiesis and partially corrects anemia in a mouse model of generalized inflammation.
    Blood. 116: 6072-81.
  2. Kuo, Y.M. et al. (2004) Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse.
    Gut. 53: 201-6.
  3. Krysiak, K. et al. (2014) Reduced Levels of Hspa9 Attenuates Stat5 Activation in Mouse B-cells.
    Exp Hematol. pii: S0301-472X(14)00817-0.
  4. Byun, M. et al. (2007) Cowpox virus exploits the endoplasmic reticulum retention pathway to inhibit MHC class I transport to the cell surface.
    Cell Host Microbe. 2: 306-15.
  5. Ripich, T. and Jessberger, R. (2011) SWAP-70 regulates erythropoiesis by controlling α4 integrin.
    Haematologica. 96: 1743-52.
  6. Hadziahmetovic, M. et al. (2012) Microarray analysis of murine retinal light damage reveals changes in iron regulatory, complement, and antioxidant genes in the neurosensory retina and isolated RPE.
    Invest Ophthalmol Vis Sci. 53 (9): 5231-41.
  7. Niewoehner, J. et al. (2014) Increased brain penetration and potency of a therapeutic antibody using a monovalent molecular shuttle.
    Neuron. 81: 49-60.
  8. Sands, S.A. et al. (2015) The habenula and iron metabolism in cerebral mouse models of multiple sclerosis.
    Neurosci Lett. 606: 204-8.
  9. View The Latest Product References
  10. Baumann, B. et al. (2017) Conditional Müller Cell Ablation Leads to Retinal Iron Accumulation.
    Invest Ophthalmol Vis Sci. 58 (10): 4223-34.
  11. Nelvagal, H.R. et al. (2020) Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.
    Sci Rep. 10 (1): 15157.
  12. Hargreaves, A. et al. (2021) Tumors modulate fenestrated vascular beds and host endocrine status.
    J Appl Toxicol. May 11 [Epub ahead of print].
  13. Zhang, K.R. et al. (2022) Conditional knockout of hephaestin in the neural retina disrupts retinal iron homeostasis.
    Exp Eye Res. : Mar 07: 109028 [Epub ahead of print].
  14. Hargreaves, A. et al. (2022) Tumours modulate the systemic vascular response to anti-angiogenic therapy.
    J Appl Toxicol. Feb 13 [Epub ahead of print].

Further Reading

  1. Lesley, J. et al. (1984) Expression of transferrin receptor on murine hematopoietic progenitors.
    Cell Immunol. 83 (1): 14-25.
  2. Trowbridge, I.S. et al. (1982) Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody.
    J Cell Physiol. 112 (3): 403-10.

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Western Blotting

Synonyms
Transferrin Receptor
RRID
AB_321758
UniProt
Q62351
Entrez Gene
Tfrc
GO Terms
GO:0005886 plasma membrane
GO:0008233 peptidase activity
GO:0005381 iron ion transmembrane transporter activity
GO:0016021 integral to membrane
GO:0004998 transferrin receptor activity
GO:0005768 endosome
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0006897 endocytosis
GO:0042470 melanosome
GO:0048471 perinuclear region of cytoplasm
GO:0070062 extracellular vesicular exosome
View more products with CD71 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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