CD71 antibody | YTA74.4

Rat anti Mouse CD71:Alexa Fluor® 647
Rat anti Mouse CD71:FITC
Rat anti Mouse CD71
Rat anti Mouse CD71:RPE
- Product Type
- Monoclonal Antibody
- Clone
- YTA74.4
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1033A647 | F | 100 Tests/1ml |
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MCA1033A647T | F | 25 Tests/0.25ml |
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MCA1033F | F | 0.1 mg |
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MCA1033G | C F IP | 0.25 mg |
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MCA1033GA | C F IP | 0.1 mg |
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MCA1033GT | C F IP | 25 µg |
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MCA1033PE | F | 100 Tests |
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Rat anti Mouse CD71 antibody, clone YTA74.4 blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (Trowbridge et al. 1982).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Concanavilin A activated mouse spleen cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q62351
- Entrez Gene
- Tfrc
- GO Terms
- GO:0005886 plasma membrane
- GO:0008233 peptidase activity
- GO:0005381 iron ion transmembrane transporter activity
- GO:0016021 integral to membrane
- GO:0004998 transferrin receptor activity
- GO:0005768 endosome
- GO:0006508 proteolysis
- GO:0006879 cellular iron ion homeostasis
- GO:0006897 endocytosis
- GO:0042470 melanosome
- GO:0048471 perinuclear region of cytoplasm
- GO:0070062 extracellular vesicular exosome
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD71 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 | |
Flow Cytometry | Neat | ||
Flow Cytometry | 1/50 | 1/100 | |
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
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Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml |
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Rat IgG2a Negative Control:FITC | MCA1212F | F | 100 Tests |
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Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Product Specific References
References for CD71 antibody
-
Millot, S. et al. (2010) Erythropoietin stimulates spleen BMP4-dependent stress erythropoiesis and partially corrects anemia in a mouse model of generalized inflammation.
Blood. 116: 6072-81. -
Kuo, Y.M. et al. (2004) Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse.
Gut. 53: 201-6. -
Krysiak, K. et al. (2014) Reduced Levels of Hspa9 Attenuates Stat5 Activation in Mouse B-cells.
Exp Hematol. pii: S0301-472X(14)00817-0. -
Byun, M. et al. (2007) Cowpox virus exploits the endoplasmic reticulum retention pathway to inhibit MHC class I transport to the cell surface.
Cell Host Microbe. 2: 306-15. -
Ripich, T. and Jessberger, R. (2011) SWAP-70 regulates erythropoiesis by controlling α4 integrin.
Haematologica. 96: 1743-52. -
Hadziahmetovic, M. et al. (2012) Microarray analysis of murine retinal light damage reveals changes in iron regulatory, complement, and antioxidant genes in the neurosensory retina and isolated RPE.
Invest Ophthalmol Vis Sci. 53 (9): 5231-41. -
Niewoehner, J. et al. (2014) Increased brain penetration and potency of a therapeutic antibody using a monovalent molecular shuttle.
Neuron. 81: 49-60. -
Sands, S.A. et al. (2015) The habenula and iron metabolism in cerebral mouse models of multiple sclerosis.
Neurosci Lett. 606: 204-8. -
Baumann, B. et al. (2017) Conditional Müller Cell Ablation Leads to Retinal Iron Accumulation.
Invest Ophthalmol Vis Sci. 58 (10): 4223-34. -
Nelvagal, H.R. et al. (2020) Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.
Sci Rep. 10 (1): 15157.
Further Reading
-
Lesley, J. et al. (1984) Expression of transferrin receptor on murine hematopoietic progenitors.
Cell Immunol. 83 (1): 14-25. -
Trowbridge, I.S. et al. (1982) Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody.
J Cell Physiol. 112 (3): 403-10.
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