CD71 antibody | YTA74.4
Rat anti Mouse CD71 antibody, clone YTA74.1 (MCA1033G) used to evaluate TfR expression in mouse retinall tissue lysates by western blotting.
Image caption:
Tfr protein levels decreased in both the 2-week MCA (K) and 5-month MCA (L) retinas compared with age-matched controls, as assessed by Western analysis. Loading control β-actin (42 kDa) bands are shown below each set of lanes. Plots of densitometry normalized to loading control calculated using FIJI software. Numbers represent mean values (±SEM). *P <0.05, **P <0.01.
From: Baumann B, Sterling J, Song Y, Song D, Fruttiger M, Gillies M, Shen W, Dunaief JL.
Conditional Müller Cell Ablation Leads to Retinal Iron Accumulation.
Invest Ophthalmol Vis Sci. 2017 Aug 1;58(10):4223-4234.
doi: 10.1167/iovs.17-21743.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB ResetRat anti Mouse CD71
- Product Type
- Monoclonal Antibody
- Clone
- YTA74.4
- Isotype
- IgG2a
- Specificity
- CD71
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD71 antibody, clone YTA74.4 recognizes the mouse Transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron. Rat anti Mouse CD71 antibody, clone YTA74.4 blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (Trowbridge et al. 1982). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Concanavilin A activated mouse spleen cells.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Rat IgG2a Negative Control | ||||||
References for CD71 antibody
-
Millot, S. et al. (2010) Erythropoietin stimulates spleen BMP4-dependent stress erythropoiesis and partially corrects anemia in a mouse model of generalized inflammation.
Blood. 116: 6072-81. -
Kuo, Y.M. et al. (2004) Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse.
Gut. 53: 201-6. -
Krysiak, K. et al. (2014) Reduced Levels of Hspa9 Attenuates Stat5 Activation in Mouse B-cells.
Exp Hematol. pii: S0301-472X(14)00817-0. -
Byun, M. et al. (2007) Cowpox virus exploits the endoplasmic reticulum retention pathway to inhibit MHC class I transport to the cell surface.
Cell Host Microbe. 2: 306-15. -
Ripich, T. and Jessberger, R. (2011) SWAP-70 regulates erythropoiesis by controlling α4 integrin.
Haematologica. 96: 1743-52. -
Hadziahmetovic, M. et al. (2012) Microarray analysis of murine retinal light damage reveals changes in iron regulatory, complement, and antioxidant genes in the neurosensory retina and isolated RPE.
Invest Ophthalmol Vis Sci. 53 (9): 5231-41. -
Niewoehner, J. et al. (2014) Increased brain penetration and potency of a therapeutic antibody using a monovalent molecular shuttle.
Neuron. 81: 49-60. -
Sands, S.A. et al. (2015) The habenula and iron metabolism in cerebral mouse models of multiple sclerosis.
Neurosci Lett. 606: 204-8.
View The Latest Product References
-
Baumann, B. et al. (2017) Conditional Müller Cell Ablation Leads to Retinal Iron Accumulation.
Invest Ophthalmol Vis Sci. 58 (10): 4223-34. -
Nelvagal, H.R. et al. (2020) Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.
Sci Rep. 10 (1): 15157. -
Hargreaves, A. et al. (2021) Tumors modulate fenestrated vascular beds and host endocrine status.
J Appl Toxicol. May 11 [Epub ahead of print]. -
Zhang, K.R. et al. (2022) Conditional knockout of hephaestin in the neural retina disrupts retinal iron homeostasis.
Exp Eye Res. : Mar 07: 109028 [Epub ahead of print]. -
Hargreaves, A. et al. (2022) Tumours modulate the systemic vascular response to anti-angiogenic therapy.
J Appl Toxicol. Feb 13 [Epub ahead of print].
Further Reading
-
Lesley, J. et al. (1984) Expression of transferrin receptor on murine hematopoietic progenitors.
Cell Immunol. 83 (1): 14-25. -
Trowbridge, I.S. et al. (1982) Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody.
J Cell Physiol. 112 (3): 403-10.
- Synonyms
- Transferrin Receptor
- RRID
- AB_321758
- UniProt
- Q62351
- Entrez Gene
- Tfrc
- GO Terms
- GO:0005886 plasma membrane
- GO:0008233 peptidase activity
- GO:0005381 iron ion transmembrane transporter activity
- GO:0016021 integral to membrane
- GO:0004998 transferrin receptor activity
- GO:0005768 endosome
- GO:0006508 proteolysis
- GO:0006879 cellular iron ion homeostasis
- GO:0006897 endocytosis
- View More GO Terms
- GO:0042470 melanosome
- GO:0048471 perinuclear region of cytoplasm
- GO:0070062 extracellular vesicular exosome
View more products with CD71 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up