CD51 antibody | RMV-7

Rat anti Mouse CD51

Product Type
Monoclonal Antibody
Clone
RMV-7
Isotype
IgG1
Specificity
CD51

Product Code Applications Pack Size List Price Your Price Qty
MCA2461GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
List Price Your Price
loader
MCA2461
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD51 antibody, clone RMV-7 recognizes murine CD51, a ~140 kDa alpha subunit of the vitronectin receptor, which is otherwise known as the integrin alpha v chain. CD51 can form heterodimers at the cell surface with a variety of beta integrins including CD29 and CD61.

Heterodimers of CD51/CD61 function as a receptor for vitronectin, and a wide array of RGD-containing proteins including fibronectin, fibrinogen, von Willebrand factor, laminin, thrombospondin and osteopontin. CD51/CD61 is primarily expressed on myeloid cells and activated T-cells. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing.

Rat anti Mouse CD51 antibody, clone RMV-7 has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity (Takahashi et al. 1990).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Cultured LAK cells from Balb/c mice.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised SD rats were fused with the cells of the P3X.63Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 Pack Size: 0.1 mg
1/25 Pack Size: 0.25 mg
1/100
Immunohistology - Frozen 1/25 1/100
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CD51 antibody

  1. Takahashi, K. et al. (1990) A murine very late activation antigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-target cell interaction.
    J Immunol. 145 (12): 4371-9.
  2. Piali, L. et al. (1995) CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium.
    J Cell Biol. 130 (2): 451-60.
  3. Cui R et al. (2007) Abrogation of the interaction between osteopontin and alphavbeta3 integrin reduces tumor growth of human lung cancer cells in mice.
    Lung Cancer. 57 (3): 302-10.
  4. Takahashi F et al. (2001) Role of osteopontin in the pathogenesis of bleomycin-induced pulmonary fibrosis.
    Am J Respir Cell Mol Biol. 24 (3): 264-71.

Further Reading

  1. Henderson, N.C. et al. (2013) Targeting of ααv integrin identifies a core molecular pathway that regulates fibrosis in several organs.
    Nat Med. 19 (12): 1617-24.

Synonyms
Integrin Alpha V
RRID
AB_931725
UniProt
P43406
Entrez Gene
Itgav
GO Terms
GO:0001525 angiogenesis
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0009897 external side of plasma membrane
GO:0030154 cell differentiation
GO:0043277 apoptotic cell clearance
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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