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CD48 antibody | OX-78

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Anti Mouse CD48 Antibody, clone OX-78 thumbnail image 1 Anti Mouse CD48 Antibody, clone OX-78 thumbnail image 2
Anti Mouse CD48 Antibody, clone OX-78 gallery image 1
Figure A. Alexa Fluor 700 conjugated Rat anti Mouse CD3 (MCA500A700) and PE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. Alexa Fluor 700 conjugated Rat anti Mouse CD3 (MCA500A700) and RPE conjugated Rat anti Mouse CD48 (MCA925PE). All experiments performed on red cell lysed mouse peripheral blood gated on live, single cell lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5™ Cell Analyzer
Anti Mouse CD48 Antibody, clone OX-78 gallery image 2
Figure A. FITC conjugated Rat anti Mouse CD45R (MCA1258F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Rat anti Mouse CD45R (MCA1258F) and RPE conjugated Rat anti Mouse CD48 (MCA925PE). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)

Rat anti Mouse CD48:FITC

Rat anti Mouse CD48

Rat anti Mouse CD48:RPE

Product Type
Monoclonal Antibody
Clone
OX-78
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA925F
Datasheet
F
MSDS
0.1 mg loader

MCA925GA
Datasheet
F
MSDS
0.1 mg loader

MCA925G
Datasheet
F
MSDS
0.25 mg loader

MCA925PE
Datasheet
F
MSDS
100 Tests loader

Rat anti Mouse CD48 antibody, clone OX-78 recognizes murine CD48 antigen, also known as BCM1 surface antigen, BLAST-1, HM48-1, MRC OX-45 surface antigen, SLAM family member 2. CD48 is a 195 amino acid GPI anchored membrane glycoprotein with an additional 22 amino acid signal peptide and a 23 amino acid propeptide region cleaved in the mature form.

Rat anti Mouse CD48 antibody, clone OX-78 labels mouse thymocytes, B and T cells and macrophages. CD48 is a ligand for the mouse CD2 cell surface antigen.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Recombinant soluble mouse CD48.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized AO Rats were fused with cells from the Rat Y3 myeloma cell line.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 0.1 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.25 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
12 months from date of reconstitution.

More Information

UniProt
P18181
Entrez Gene
Cd48
GO Terms
GO:0005515 protein binding
GO:0007165 signal transduction
GO:0009897 external side of plasma membrane
GO:0042110 T cell activation
GO:0043499 eukaryotic cell surface binding
GO:0051789 response to protein stimulus
Regulatory
For research purposes only

Applications of CD48 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Product Specific References

References for CD48 antibody

  1. Kato, K. et al. (1992) CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation.
    J Exp Med. 176 (5): 1241-9.
  2. Lapaque, N. et al. (2006) Characterization of Brucella abortus lipopolysaccharide macrodomains as mega rafts.
    Cell Microbiol. 8 (2): 197-206.
  3. Kashiwakura, Y. et al. (2013) CD2-mediated regulation of peripheral CD4(+)  CD25(+) regulatory T-cell apoptosis accompanied by down-regulation of Bim.
    Immunology. 139 (1): 48-60.
  4. Wild, M.K. et al. (1999) Dependence of T cell antigen recognition on the dimensions of an accessory receptor-ligand complex.
    J Exp Med. 190: 31-41.
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