CD48 antibody | OX-78

Rat anti Mouse CD48:RPE

Product Type
Monoclonal Antibody
Clone
OX-78
Isotype
IgG2a
Specificity
CD48

Product Code Applications Pack Size List Price Your Price Qty
MCA925PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Rat anti Mouse CD48 antibody, clone OX-78 recognizes murine CD48 antigen, also known as BCM1 surface antigen, BLAST-1, HM48-1, MRC OX-45 surface antigen, SLAM family member 2. CD48 is a 195 amino acid GPI anchored membrane glycoprotein with an additional 22 amino acid signal peptide and a 23 amino acid propeptide region cleaved in the mature form.

Rat anti Mouse CD48 antibody, clone OX-78 labels mouse thymocytes, B and T cells and macrophages. CD48 is a ligand for the mouse CD2 cell surface antigen.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Recombinant soluble mouse CD48.
Fusion Partners
Spleen cells from immunized AO Rats were fused with cells from the Rat Y3 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests loader
List Price Your Price
loader
Description Rat IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD48 antibody

  1. Kato, K. et al. (1992) CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation.
    J Exp Med. 176 (5): 1241-9.
  2. Lapaque, N. et al. (2006) Characterization of Brucella abortus lipopolysaccharide macrodomains as mega rafts.
    Cell Microbiol. 8 (2): 197-206.
  3. Kashiwakura, Y. et al. (2013) CD2-mediated regulation of peripheral CD4(+)  CD25(+) regulatory T-cell apoptosis accompanied by down-regulation of Bim.
    Immunology. 139 (1): 48-60.
  4. Wild, M.K. et al. (1999) Dependence of T cell antigen recognition on the dimensions of an accessory receptor-ligand complex.
    J Exp Med. 190: 31-41.
  5. Grassinger, J. et al. (2010) Phenotypically identical hemopoietic stem cells isolated from different regions of bone marrow have different biologic potential.
    Blood. 116 (17): 3185-96.
  6. Oh, J. et al. (2018) MARCH1 protects the lipid raft and tetraspanin web from MHCII proteotoxicity in dendritic cells.
    J Cell Biol. 217 (4): 1395-410.

Synonyms
BLAST-1
RRID
AB_321441
UniProt
P18181
Entrez Gene
Cd48
GO Terms
GO:0005515 protein binding
GO:0007165 signal transduction
GO:0009897 external side of plasma membrane
GO:0042110 T cell activation
GO:0043499 eukaryotic cell surface binding
GO:0051789 response to protein stimulus

MCA925PE

151258

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