CD45.2 antibody | r104-2
Rat anti Mouse CD45.2
- Product Type
- Monoclonal Antibody
- Clone
- r104-2
- Isotype
- IgG2a
- Specificity
- CD45.2
Antibody Quality and Performance Guarantee
TZA0160GA
Recombinant Antibodies
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| Rat anti Mouse CD45.2 antibody, clone r104-2, is a recombinant monoclonal antibody that recognizes mCD45.2, also known as Ly5.2. CD45.2 is an alloantigen of CD45, a type I transmembrane protein tyrosine kinase exclusively expressed on all nucleated cells of the hematopoietic system. CD45.2 is the predominant alloantigen in mice, expressed by leukocytes of most mouse strains, including A, AKR, BALB⁄c, CBA⁄Ca, CBA⁄J, C3H⁄He, C57BL, C57BR, C57L, C58, DBA⁄1, DBA⁄2, NZB, SWR, and 129. CD45 and its isoforms have a key role in initiating T cell receptor signaling by controlling the activation of Src family protein-tyrosine kinases Lck and Fyn. Ultimately this leads to increased cytokine production and proliferation of T cells. The CD45.2 alloantigen in mice differs from the CD45.1 alloantigen by only five amino acids in the extracellular domain (Zebedee 1991). CD45.2 and CD45.1 antibodies are used to track hematopoietic donor cells following mouse bone marrow transplant. Rat anti Mouse CD45.2, clone r104-2, is derived from clone 104-2, generated by cloning the variable DNA sequences into expression vectors for recombinant protein expression. |
- Target Species
- Mouse
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- thymocytes and splenocytes from B10.S mice
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
Source Hybridoma Reference
-
Shen, F.W. (1981) Monoclonal antibodies to mouse lymphocyte differentiation alloantigens.
in Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. Edited by Hämmerling, G.J, et al. Elsevier/North-Holland Biomedical Press. 1981: 25-31
- UniProt
- P06800
- Entrez Gene
- Ptprc
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
- GO:0051726 regulation of cell cycle
- GO:0004725 protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- View More GO Terms
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0007159 leukocyte cell-cell adhesion
- GO:0008201 heparin binding
- GO:0009897 external side of plasma membrane
- GO:0019901 protein kinase binding
- GO:0030183 B cell differentiation
- GO:0030890 positive regulation of B cell proliferation
- GO:0031953 negative regulation of protein autophosphorylation
- GO:0034113 heterotypic cell-cell adhesion
- GO:0042098 T cell proliferation
- GO:0042100 B cell proliferation
- GO:0043065 positive regulation of apoptosis
- GO:0043395 heparan sulfate proteoglycan binding
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045059 positive thymic T cell selection
- GO:0045060 negative thymic T cell selection
- GO:0045121 membrane raft
- GO:0045577 regulation of B cell differentiation
- GO:0045588 positive regulation of gamma-delta T cell differentiation
- GO:0046641 positive regulation of alpha-beta T cell proliferation
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050855 regulation of B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
View more products with CD45 specificity
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