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CD43 antibody | 1B11

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Rat anti Mouse CD43

Product Type
Monoclonal Antibody
Clone
1B11
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA4702GA
Datasheet
C F IP WB
SDS
0.1 mg loader

Rat anti Mouse CD43 antibody, clone 1B11 recognizes mouse CD43, one of the major glycoproteins of thymocytes and T lymphocytes. It has a high content of sialic acid and O-linked carbohydrate groups. CD43 presents carbohydrate lligands to selectins, plays a role in lectin binding and is a counter receptor for Siglec-1.

CD43 is expressed on T lymphocytes in two predominant glycoforms. In its ~115 kDa form it is a pan T cell marker, whereas in its ~130 kDa form it is associated with T cell activation. Rat anti Mouse CD43 antibody, clone 1B11 specifically recognizes the 130 kDa glycoform.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Mouse WEHI 274.3 myeloid tumour cells.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P15702
Entrez Gene
Spn
GO Terms
GO:0005515 protein binding
GO:0001562 response to protozoan
GO:0001808 negative regulation of type IV hypersensitivity
GO:0001931 uropod
GO:0042742 defense response to bacterium
GO:0005887 integral to plasma membrane
GO:0005604 basement membrane
GO:0007162 negative regulation of cell adhesion
GO:0007166 cell surface receptor linked signaling pathway
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0031295 T cell costimulation
GO:0042102 positive regulation of T cell proliferation
GO:0042130 negative regulation of T cell proliferation
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0045060 negative thymic T cell selection
GO:0050688 regulation of defense response to virus
GO:0051635 bacterial cell surface binding
Regulatory
For research purposes only

Applications of CD43 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader

Product Specific References

References for CD43 antibody

  1. Barran, P. et al. (1997) Modification of CD43 and other lymphocyte O-glycoproteins by core 2 N-acetylglucosaminyltransferase.
    Glycobiology. 7 (1): 129-36.

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