CD43 antibody | 1B11
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Rat anti Mouse CD43 antibody, clone 1B11 recognizes mouse CD43, one of the major glycoproteins of thymocytes and T lymphocytes. It has a high content of sialic acid and O-linked carbohydrate groups. CD43 presents carbohydrate lligands to selectins, plays a role in lectin binding and is a counter receptor for Siglec-1.
CD43 is expressed on T lymphocytes in two predominant glycoforms. In its ~115 kDa form it is a pan T cell marker, whereas in its ~130 kDa form it is associated with T cell activation. Rat anti Mouse CD43 antibody, clone 1B11 specifically recognizes the 130 kDa glycoform.
CD43 is expressed on T lymphocytes in two predominant glycoforms. In its ~115 kDa form it is a pan T cell marker, whereas in its ~130 kDa form it is associated with T cell activation. Rat anti Mouse CD43 antibody, clone 1B11 specifically recognizes the 130 kDa glycoform.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Mouse WEHI 274.3 myeloid tumour cells.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P15702 Related reagents
- Entrez Gene
- Spn Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0001562 response to protozoan
- GO:0001808 negative regulation of type IV hypersensitivity
- GO:0001931 uropod
- GO:0042742 defense response to bacterium
- GO:0005887 integral to plasma membrane
- GO:0005604 basement membrane
- GO:0007162 negative regulation of cell adhesion
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0009897 external side of plasma membrane
- GO:0031295 T cell costimulation
- GO:0042102 positive regulation of T cell proliferation
- GO:0042130 negative regulation of T cell proliferation
- GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
- GO:0045060 negative thymic T cell selection
- GO:0050688 regulation of defense response to virus
- GO:0051635 bacterial cell surface binding
- Regulatory
- For research purposes only
Applications of CD43 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/5 | 1/10 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | 1 ml | C E P WB | ||
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | 0.5 mg | C E IF P WB | ||
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | 0.1 mg | F IF WB | ||
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | 1 mg | F | ||
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | 1 mg | C E P RE | ||
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | 0.5 ml | F | ||
| Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) | STAR71D549GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) | STAR71D649GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | 0.1 mg | F IF WB | ||
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | 0.5 mg | C E P | ||
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | 0.5 ml | F |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | 1 ml | E F |
Product Specific References
References for CD43 antibody
-
Barran, P. et al. (1997) Modification of CD43 and other lymphocyte O-glycoproteins by core 2 N-acetylglucosaminyltransferase.
Glycobiology. 7 (1): 129-36.
