Rat anti Mouse CD43 antibody, clone 1B11 recognizes mouse CD43, one of the major glycoproteins of thymocytes and T lymphocytes. It has a high content of sialic acid and O-linked carbohydrate groups. CD43 presents carbohydrate lligands to selectins, plays a role in lectin binding and is a counter receptor for Siglec-1.
CD43 is expressed on T lymphocytes in two predominant glycoforms. In its ~115 kDa form it is a pan T cell marker, whereas in its ~130 kDa form it is associated with T cell activation. Rat anti Mouse CD43 antibody, clone 1B11 specifically recognizes the 130 kDa glycoform.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Mouse WEHI 274.3 myeloid tumour cells.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
response to protozoan
negative regulation of type IV hypersensitivity
defense response to bacterium
integral to plasma membrane
negative regulation of cell adhesion
cell surface receptor linked signaling pathway
induction of apoptosis by extracellular signals
external side of plasma membrane
T cell costimulation
positive regulation of T cell proliferation
negative regulation of T cell proliferation
positive regulation of tumor necrosis factor biosynthetic process
negative thymic T cell selection
regulation of defense response to virus
bacterial cell surface binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD43 antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD43 antibody
Barran, P. et al. (1997) Modification of CD43 and other lymphocyte O-glycoproteins by core 2 N-acetylglucosaminyltransferase.
Glycobiology. 7 (1): 129-36.
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