CD43 antibody | 1B11
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Rat anti Mouse CD43
- Product Type
- Monoclonal Antibody
- Clone
- 1B11
- Isotype
- IgG2a
- Specificity
- CD43
Antibody Quality and 
Unrivaled 
| Rat anti Mouse CD43 antibody, clone 1B11 recognizes mouse CD43, one of the major glycoproteins of thymocytes and T lymphocytes. It has a high content of sialic acid and O-linked carbohydrate groups. CD43 presents carbohydrate lligands to selectins, plays a role in lectin binding and is a counter receptor for Siglec-1. CD43 is expressed on T lymphocytes in two predominant glycoforms. In its ~115 kDa form it is a pan T cell marker, whereas in its ~130 kDa form it is associated with T cell activation. Rat anti Mouse CD43 antibody, clone 1B11 specifically recognizes the 130 kDa glycoform. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse WEHI 274.3 myeloid tumour cells.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/5 | 1/10 |
| Immunohistology - Frozen | |
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| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for CD43 antibody
-
Barran, P. et al. (1997) Modification of CD43 and other lymphocyte O-glycoproteins by core 2 N-acetylglucosaminyltransferase.
Glycobiology. 7 (1): 129-36.
- Synonyms
- Leukosialin
- RRID
- AB_2194191
- UniProt
- P15702
- Entrez Gene
- Spn
- GO Terms
- GO:0005515 protein binding
- GO:0001562 response to protozoan
- GO:0001808 negative regulation of type IV hypersensitivity
- GO:0001931 uropod
- GO:0042742 defense response to bacterium
- GO:0005887 integral to plasma membrane
- GO:0005604 basement membrane
- GO:0007162 negative regulation of cell adhesion
- GO:0007166 cell surface receptor linked signaling pathway
- View More GO Terms
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0009897 external side of plasma membrane
- GO:0031295 T cell costimulation
- GO:0042102 positive regulation of T cell proliferation
- GO:0042130 negative regulation of T cell proliferation
- GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
- GO:0045060 negative thymic T cell selection
- GO:0050688 regulation of defense response to virus
- GO:0051635 bacterial cell surface binding
View more products with CD43 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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