Rat IgG2a Negative Control antibody
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|Rat IgG2a Negative Control antibody is suitable for the assessment of the level of non-specific binding of rat IgG2a monoclonal antibodies to mouse cells.
Test results indicate Rat IgG2a Negative Control antibody is also suitable for use as a negative control with canine cells.
N.B. This antibody recognizes a human cell surface marker, and therefore is not suitable as a negative control in human cells or cell lines.
- Target Species
- Negative Control
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Human lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized DA rats were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) Pacific Blue® 410 455
- For research purposes only
- 12 months from date of despatch
- The Pacific Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
* It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Rat IgG2a Negative Control antibody
Sumagin, R. et al. (2008) Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling.
Am J Physiol Heart Circ Physiol. 295: H969-H977.
Chiu, W.C. et al. (2011) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.
J Nutr Biochem. 20: 254-60.
Guilloteau, L.A. et al. (2003) Nramp1 is not a major determinant in the control of Brucella melitensis infection in mice.
Infect Immun. 71: 621-8.
Stapleton, T.W. et al. (2000) Investigation of the regenerative capacity of an acellular porcine medial meniscus for tissue engineering applications.
Tissue Eng Part A. 17: 231-42.
Park, S.W. et al. (2012) A1 adenosine receptor allosteric enhancer PD-81723 protects against renal ischemia-reperfusion injury.
Am J Physiol Renal Physiol. 303: F721-32.
Schmidt, E.P. et al. (2012) The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.
Nat Med. 18 (8): 1217-23.
McConnell, M.J. et al. (2009) H2-K(b) and H2-D(b) regulate cerebellar long-term depression and limit motor learning.
Proc Natl Acad Sci U S A. 106: 6784-9.
Rabadi, M. et al. (2016) Peptidyl arginine deiminase-4-deficient mice are protected against kidney and liver injury after renal ischemia and reperfusion.
Am J Physiol Renal Physiol. 311 (2): F437-49.
View The Latest Product References
Rabadi, M.M. et al. (2019) Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO (NFκB Essential Modulator).
Am J Physiol Renal Physiol. Apr 03 [Epub ahead of print].
Han, S.J. et al. (2020) Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injury.
JCI Insight. 5 (19)Sep 17 [Epub ahead of print].
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