CD335 antibody | 29A1.4
|Rat anti Mouse CD335 antibody, clone 29A1.4 recognizes natural killer cell p46-related protein (NKp46), otherwise known as CD335, which is uniquely expressed by resting and activated natural killer (NK) cells, but no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.
It is a major NK cell lysis receptor for autologous pathogen-infected and tumor target cells during natural cytotoxicity responses. Ligands of CD335 include viral hemagglutinins (HAs) and heparan sulfate proteoglycans (HSPGs) on the surface of tumor cells.
Staining with Rat anti mouse CD335 (29A1.4) is not strain specific and the antibody has been used to stain C57BL/6, SJL, CBA/CA and BALB/c strains. Clone 29A1.4 also activates NK cells in vitro. It does not deplete NK cells in vivo.
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- NKP46-IgG1 Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD335 antibody
Ota, N. et al. (2011) IL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection with Citrobacter rodentium.
Nat Immunol. 12 (10): 941-8.
Walzer, T. et al. (2007) Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46.
Proc Natl Acad Sci U S A. 104: 3384-9.
Walzer, T. et al. (2007) Natural killer cell trafficking in vivo requires a dedicated sphingosine 1-phosphate receptor.
Nat Immunol. 8 (12): 1337-44.
Luci, C. et al. (2009) Influence of the transcription factor RORgammat on the development of NKp46+ cell populations in gut and skin.
Nat Immunol. 10: 75-82.
Chiossone, L. et al. (2009) Maturation of mouse NK cells is a 4-stage developmental program.
Blood. 113: 5488-96.
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