CD335 antibody | 29A1.4

Rat anti Mouse CD335

Product Type
Monoclonal Antibody
Clone
29A1.4
Isotype
IgG2a
Specificity
CD335

Product Code Applications Pack Size List Price Your Price Qty
MCA5938GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
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Rat anti Mouse CD335 antibody, clone 29A1.4 recognizes natural killer cell p46-related protein (NKp46), otherwise known as CD335, which is uniquely expressed by resting and activated natural killer (NK) cells, but no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.

It is a major NK cell lysis receptor for autologous pathogen-infected and tumor target cells during natural cytotoxicity responses. Ligands of CD335 include viral hemagglutinins (HAs) and heparan sulfate proteoglycans (HSPGs) on the surface of tumor cells.

Staining with Rat anti mouse CD335 (29A1.4) is not strain specific and the antibody has been used to stain C57BL/6, SJL, CBA/CA and BALB/c strains. Clone 29A1.4 also activates NK cells in vitro. It does not deplete NK cells in vivo.

Target Species
Mouse
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
NKP46-IgG1 Fc fusion protein.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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Description Rat IgG2a Negative Control

References for CD335 antibody

  1. Ota, N. et al. (2011) IL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection with Citrobacter rodentium.
    Nat Immunol. 12 (10): 941-8.
  2. Walzer, T. et al. (2007) Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46.
    Proc Natl Acad Sci U S A. 104: 3384-9.
  3. Walzer, T. et al. (2007) Natural killer cell trafficking in vivo requires a dedicated sphingosine 1-phosphate receptor.
    Nat Immunol. 8 (12): 1337-44.
  4. Luci, C. et al. (2009) Influence of the transcription factor RORgammat on the development of NKp46+ cell populations in gut and skin.
    Nat Immunol. 10: 75-82.
  5. Chiossone, L. et al. (2009) Maturation of mouse NK cells is a 4-stage developmental program.
    Blood. 113: 5488-96.

Flow Cytometry

Synonyms
NKp46
UniProt
Q8C567
Entrez Gene
Ncr1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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