CD321 antibody | H202-106
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F ResetRat anti Mouse CD321
- Product Type
- Monoclonal Antibody
- Clone
- H202-106
- Isotype
- IgG1
- Specificity
- CD321
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD321 antibody, clone H202-106 recognizes murine CD321, also known as junctional adhesion molecule 1 (JAM-1). CD321 is a 274 amino acid ~32-4 1kDa single pass, type I transmembrane glycoprotein, which shares similarities with related proteins JAM-2 and JAM-3. CD321 is a multifunctional protein primarily expressed by platelets, endothelial and epithelial cells. The CD321 protein co-localises with tight junction molecules in both epithelial and endothelial cells and plays an important role in the regulation of junctional integrity and permeability. In addition, CD321 is a ligand for the integrin LFA-1 and is also involved in the transmigration of leucocytes. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- MTE1/MTE2 stromal cell lines.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized Lou rats were fused with cells of the X63 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD321 antibody
-
Malergue, F. et al. (1998) A novel immunoglobulin superfamily junctional molecule expressed by antigen presenting cells, endothelial cells and platelets.
Mol Immunol. 35 (17): 1111-9. -
Aurrand-lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members.
Blood. 98 (13): 3699-707. -
Ebnet, K. et al. (2000) Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1.
J Biol Chem. 275 (36): 27979-88. -
Prestwich, R.J. et al. (2009) Immune-mediated antitumor activity of reovirus is required for therapy and is independent of direct viral oncolysis and replication.
Clin Cancer Res. 15 (13): 4374-4381. -
Morita, Y. et al. (2010) Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment.
J Exp Med. 207 (6): 1173-82. -
Schmitt, M.M. et al. (2014) Endothelial junctional adhesion molecule-a guides monocytes into flow-dependent predilection sites of atherosclerosis.
Circulation. 129 (1): 66-76. -
Narni-Mancinelli, E. et al.. (2017) Complement factor P is a ligand for the natural killer cell-activating receptor NKp46.
Sci Immunol; 2(10): eaam9628. -
Nagatake, T. et al. (2020) Selective expression of claudin-5 in thymic endothelial cells regulates the blood-thymus barrier and T-cell export.
Int Immunol. Oct 10 dxaa069 [Epub ahead of print].
View The Latest Product References
- Synonyms
- JAM-1
- RRID
- AB_567266
- UniProt
- O88792
- Entrez Gene
- F11r
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005923 tight junction
- GO:0030855 epithelial cell differentiation
- GO:0044419 interspecies interaction between organisms
MCA2346
148182
MCA2346GA
150827
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