CD31 antibody | ER-MP12

Rat anti Mouse CD31:RPE

Product Type
Monoclonal Antibody
Clone
ER-MP12
Isotype
IgG2a
Specificity
CD31

Product Code Applications Pack Size List Price Your Price Qty
MCA2388PE
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F 100 Tests loader
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Rat anti Mouse CD31 antibody, clone ER-MP12 recognizes mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.

CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
BALB/c macrophage precursor cell hybrids
Fusion Partners
Cells from immunised rats were fused with the cells of the rat Y3-Ag1.2.3 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

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Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests loader
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Description Rat IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

References for CD31 antibody

  1. Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
    Eur J Immunol. 20 (1): 27-34.
  2. van der Loo, J. et al. (1995) Identification of hematopoietic stem cell subsets on the basis of their primitiveness using antibody ER-MP12.
    Blood. 85:952-62.
  3. Ling, V. et al. (1997) Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31).
    Eur J Immunol. 27:509-14.
  4. de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.
    J Immunol Methods. 217 (1-2): 27-39.
  5. Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
    Am J Pathol. 158 (1): 131-45.
  6. van Rijt, L. et al. (2002) Allergen-induced accumulation of airway dendritic cells is supported by an increase in CD31(hi)Ly-6C(neg) bone marrow precursors in a mouse model of asthma.
    Blood. 100:3663-71.
  7. Tagoh, H. et al. (2002) Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells.
    Genes Dev. 16:1721-37.
  8. Nikolic, T. et al. (2002) Developmental stages of myeloid dendritic cells in mouse bone marrow.
    Int Immunol. 15:515-24.
  9. View The Latest Product References
  10. Baumeister, T. et al. (2003) Interleukin-3Ralpha+ myeloid dendritic cells and mast cells develop simultaneously from different bone marrow precursors in cultures with interleukin-3.
    J Invest Dermatol. 121: 280-8.
  11. Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
    J Leukoc Biol. 78: 845-52.
  12. Revermann, M. et al. (2010) Soluble epoxide hydrolase deficiency attenuates neointima formation in the femoral cuff model of hyperlipidemic mice.
    Arterioscler Thromb Vasc Biol. 30: 909-14.
  13. Sumagin, R. and Sarelius, I.H. (2010) Intercellular adhesion molecule-1 enrichment near tricellular endothelial junctions is preferentially associated with leukocyte transmigration and signals for reorganization of these junctions to accommodate leukocyte passage.
    J Immunol. 184: 5242-52.
  14. Ross, E.A. et al. (2011) CD31 Is Required on CD4+ T Cells To Promote T Cell Survival during Salmonella Infection.
    J Immunol. 187: 1553-65.
  15. Thum, T. et al. (2011) Impairment of endothelial progenitor cell function and vascularization capacity by aldosterone in mice and humans.
    Eur Heart J. 32: 1275-86.
  16. Thorp, E. et al. (2011) A reporter for tracking the UPR in vivo reveals patterns of temporal and cellular stress during atherosclerotic progression.
    J Lipid Res. 52 (5): 1033-8.
  17. Schledzewski, K. et al. (2011) Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors.
    J Clin Invest. 121: 703-14.
  18. Loureiro, J. et al. (2011) Blocking TGF-{beta}1 Protects the Peritoneal Membrane from Dialysate-Induced Damage.
    J Am Soc Nephrol. 22: 1682-95.
  19. Matsakas, A. et al. (2012) Exercise training attenuates the hypermuscular phenotype and restores skeletal muscle function in the myostatin null mouse.
    Exp Physiol. 97 (1): 125-40.
  20. Trottier MD et al. (2012) Enhancement of hematopoiesis and lymphopoiesis in diet-induced obese mice.
    Proc Natl Acad Sci U S A. 109 (20): 7622-9.
  21. Moen, I. et al. (2012) Gene expression in tumor cells and stroma in dsRed 4T1 tumors in eGFP-expressing mice with and without enhanced oxygenation.
    BMC Cancer. 12: 21.
  22. Trottier, M.D. et al. (2012) Enhanced production of early lineages of monocytic and granulocytic cells in mice with colitis
    Proc Natl Acad Sci U S A.109: 16594-9.
  23. Kroon, P. et al. (2013) JAK-STAT blockade inhibits tumor initiation and clonogenic recovery of prostate cancer stem-like cells.
    Cancer Res. 73 (16): 5288-98.
  24. Ono, N. et al. (2014) A subset of chondrogenic cells provides early mesenchymal progenitors in growing bones.
    Nat Cell Biol. 16 (12): 1157-67.
  25. Eskilsson, A. et al. (2014) Distribution of microsomal prostaglandin E synthase-1 in the mouse brain.
    J Comp Neurol. 522 (14): 3229-44.
  26. Nakamura, Y. et al. (2015) Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration.
    FEBS Lett. 589 (11): 1257-65.
  27. Fraccarollo, D. et al. (2015) Efficacy of mineralocorticoid receptor antagonism in the acute myocardial infarction phase: eplerenone versus spironolactone.
    ESC Heart Fail. 2 (3): 150-8.
  28. Stein-Merlob, A.F. et al. (2015) Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus Age-Dependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study.
    Theranostics. 5 (12): 1317-27.
  29. Cao Y et al. (2016) IL-1β differently stimulates proliferation and multinucleation of distinct mouse bone marrow osteoclast precursor subsets.
    J Leukoc Biol. 100 (3): 513-23.
  30. Ryan, T.E. et al. (2016) Mitochondrial therapy improves limb perfusion and myopathy following hindlimb ischemia.
    J Mol Cell Cardiol. 97: 191-6.
  31. Shi, H. et al. (2016) Hiding inside? Intracellular expression of non-glycosylated c-kit protein in cardiac progenitor cells.
    Stem Cell Res. 16 (3): 795-806.
  32. Chowdhury, B. et al. (2016) Hyaluronidase 2 (HYAL2) is expressed in endothelial cells, as well as some specialized epithelial cells, and is required for normal hyaluronan catabolism.
    Histochem Cell Biol. 145 (1): 53-66.
  33. Yip, H.K. et al. (2016) Tissue plasminogen activator deficiency preserves neurological function and protects against murine acute ischemic stroke.
    Int J Cardiol. 205: 133-41.
  34. Reigstad, I. et al. (2016) The Effect of Stromal Integrin β3-Deficiency on Two Different Tumors in Mice.
    Cancers (Basel). 8 (1): pii: E14.
  35. Bongiorno, E.K. et al. (2017) Type 1 Immune Mechanisms Driven by the Response to Infection with Attenuated Rabies Virus Result in Changes in the Immune Bias of the Tumor Microenvironment and Necrosis of Mouse GL261 Brain Tumors.
    J Immunol. 198 (11): 4513-4523.
  36. Cao, Y. et al. (2017) TNF-α has both stimulatory and inhibitory effects on mouse monocyte-derived osteoclastogenesis.
    J Cell Physiol. 232 (12): 3273-85.
  37. Piro, J.R. et al. (2018) Inhibition of 2-AG hydrolysis differentially regulates blood brain barrier permeability after injury.
    J Neuroinflammation. 15 (1): 142.
  38. Oikawa, S. et al. (2018) Role of endothelial microRNA-23 clusters in angiogenesis in vivo.
    Am J Physiol Heart Circ Physiol. 315 (4): H838-H846.
  39. Tay, M.H.D. et al. (2019) Halted Lymphocyte Egress via Efferent Lymph Contributes to Lymph Node Hypertrophy During Hypercholesterolemia.
    Front Immunol. 10: 575.
  40. Iring, A. et al. (2019) Shear stress-induced endothelial adrenomedullin signaling regulates vascular tone and blood pressure.
    J Clin Invest. 129 (7): 2775-91.
  41. Ascone, G. et al. (2020) Increase in the Number of Bone Marrow Osteoclast Precursors at Different Skeletal Sites, Particularly in Long Bone and Jaw Marrow in Mice Lacking IL-1RA.
    Int J Mol Sci. 21(11):3774.
  42. Patel, P. et al. (2023) Development of a Personalised Device for Systemic Magnetic Drug Targeting to Brain Tumours.
    Nanotheranostics. 7 (1): 102-16.
  43. Eskilsson, A. et al. (2023) Prostaglandin production in brain endothelial cells during the initiation of fever.
    Commun Integr Biol. 16 (1): 2166237.

Flow Cytometry

Immunofluorescence

Synonyms
PECAM-1
RRID
AB_1055663
UniProt
Q08481
Entrez Gene
Pecam1
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005625 soluble fraction
GO:0007266 Rho protein signal transduction
GO:0030054 cell junction
GO:0030334 regulation of cell migration
GO:0030485 smooth muscle contractile fiber
GO:0042060 wound healing
GO:0045121 membrane raft
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