CD31 antibody | ER-MP12
Rat anti Mouse CD31 antibody, clone ER-MP12 (MCA2388) used for the detection of blood vessels in an experimental murine tumor model by immunohistofluorescence.
Image caption:
Percentage of pixels positive for NG2 in 4T1 (n = 5) and RM11 (n = 4 and n = 6) tumors (A,D) from WT and β3-KO mice were calculated using immunofluorescent images. No statistical differences in 4T1 (p = 0.90) or RM11 (p = 0.23) were found. Mean ± SD. Representative images of NG2-staining from both genotypes of 4T1 (B,C) (NG2 green, CD31 red) and RM11 (E,F) (NG2 red) tumors are shown. Scale bars indicate 50 μm.
From: Reigstad I, Sortland K, Skogstrand T, Reed RK, Stuhr L.
The Effect of Stromal Integrin β3-Deficiency on Two Different Tumors in Mice.
Cancers (Basel). 2016 Jan 12;8(1). pii: E14.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD31 antibody, clone ER-MP12 (MCA2388) used for the demonstration of vasculature in mouse brain by immunofluorescence.
Image caption:
Inhibition of 2-AG hydrolysis reduces LPS-induced BBB permeability. a, b Fibrinogen levels in b plasma and the a ratio of brain to plasma fibrinogen were assessed by ELISA. n = 5/7 mice per group. c, d Fluorescent immunostaining in the striatum for fibrinogen (red) and vascular marker (CD31; green) demonstrated leakage of fibrinogen into the brain with vehicle treatment, whereas vascular integrity was preserved when (e, f) MAGL was inhibited. g Extravascular fibrinogen was semi-quantitated in fluorescently labeled sections of the striatum. Bar graphs were plotted with mean ±SEM and data analyzed using one-way analysis of variance (ANOVA) with Tukey post-hoc comparisons. n = 5/7 mice per group. Significance is shown as *p <0.05, **p <0.01. Scale bar = 20 μm.
From: Piro JR, Suidan GL, Quan J, Pi Y, O'Neill SM, Ilardi M, Pozdnyakov N, Lanz TA, Xi H, Bell RD, Samad TA.
Inhibition of 2-AG hydrolysis differentially regulates blood brain barrier permeability after injury.
J Neuroinflammation. 2018 May 14;15(1):142.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD31 antibody, clone ER-MP12 (MCA2388B) used for the identification of osteoclast precursor populations by flow cytometry in conjunction with PE conjugated Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MCA2389PE).
Image caption:
Identification of different subsets of osteoclast precursors in bone marrow from long bone, calvaria, vertebra and jaw in WT and Il1rn-/-mice.
Cells were labeled with anti-CD31 and -Ly-6C and osteoclast precursor subsets were gated as: early blasts (CD31hi Ly-6C-), myeloid blasts (CD31+ Ly-6C+), and monocytes (CD31- Ly-6Chi) as encircled in each profile. Other populations mainly contained lymphocytes (Labeled A), erythroid blasts (Labeled B) and granulocytes (Labeled C).
From: Ascone G, Cao Y, Jansen IDC, Di Ceglie I, van den Bosch MHJ, Blom AB, van Lent PLEM, Everts V, de Vries TJ.
Increase in the Number of Bone Marrow Osteoclast Precursors at Different Skeletal Sites, Particularly in Long Bone and Jaw Marrow in Mice Lacking IL-1RA.
Int J Mol Sci. 2020 May 27;21(11):3774.
doi: 10.3390/ijms21113774.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD31 antibody, clone ER-MP12 (MCA2388) used to demonstrate CD31 staining in murine lymph nodes by immunofluorescence.
Image caption:
The extended lymphatic network in apoE−/− mice LNs exhibits structural abnormalities.
LYVE-1 and CD31 was examined in LN sections from apoE−/− mice at 22–28 weeks of age. Images are representative of 3–4 independent experiments (n = 3–5 mice per group). Arrows indicate loss of LYVE-1 expression.
From: Tay MHD, Lim SYJ, Leong YFI, Thiam CH, Tan KW, Torta FT, Narayanaswamy P, Wenk M, Angeli V.
Halted Lymphocyte Egress via Efferent Lymph Contributes to Lymph Node Hypertrophy During Hypercholesterolemia.
Front Immunol. 2019 Mar 27;10:575.
doi: 10.3389/fimmu.2019.00575.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD31 antibody, clone ER-MP12 (MCA2388) used to label tumor vasculature in mouse brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Magnetic guidance of MNP does not affect tumour vascularity or necrosis. Mice underwent intracranial surgery followed by injection of luciferin labelled CT-2A BT cells into the cortical region.
Sections of the brain (8μm) were stained with CD31 to assess the vessel necrosis following treatment.
From: Patel P, Alghamdi A, Shaw G, Legge C, Glover M, Freeman D, Hodgetts H, Wilson E, Howard F, Staniland S, Kennerley AJ, Wood D, Moorehead R, Hadfield C, Rominiyi O, Griffin J, Collis SJ, Hyde S, Crossley M, Paley M, Muthana M.
Development of a Personalised Device for Systemic Magnetic Drug Targeting to Brain Tumours.
Nanotheranostics. 2023 Jan 1;7(1):102-16.
doi: 10.7150/ntno.76559.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD31 antibody, clone ER-MP12 (MCA2388GA) used to label blood vessels in mouse brain by immunofluorescence.
Image caption:
Prostaglandin synthesis in the brain during the initial febrile response to i.v. injection of LPS.
c-e. Confocal micrographs showing induced Cox-2 immunoreactivity (red) in large (c) and small (d) blood vessels, stained for the endothelial cell marker CD31 (green), 30 min after LPS injection. After saline injection (e), there was no Cox-2 in the blood vessels, however, staining of cortical neurons (arrowheads) was seen, demonstrating method accuracy. Scale bar = 20 μm.
From: Eskilsson A, Shionoya K, Blomqvist A.
Prostaglandin production in brain endothelial cells during the initiation of fever.
Commun Integr Biol. 2023 Jan 11;16(1):2166237.
doi: 10.1080/19420889.2023.2166237.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD31
- Product Type
- Monoclonal Antibody
- Clone
- ER-MP12
- Isotype
- IgG2a
- Specificity
- CD31
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD31 antibody, clone ER-MP12 recognizes mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations. CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- BALB/c macrophage precursor cell hybrids
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with the cells of the rat Y3-Ag1.2.3 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 Pack Size: 0.25 mg, 25 µg 1/10 Pack Size: 0.1 mg |
1/200 Pack Size: 0.25 mg, 25 µg 1/100 Pack Size: 0.1 mg |
| Immunofluorescence | |
||
| Immunohistology - Frozen 1 | |
1/10 | 1/100 |
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for CD31 antibody
-
Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
Eur J Immunol. 20 (1): 27-34. -
van der Loo, J. et al. (1995) Identification of hematopoietic stem cell subsets on the basis of their primitiveness using antibody ER-MP12.
Blood. 85:952-62. -
Ling, V. et al. (1997) Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31).
Eur J Immunol. 27:509-14. -
de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.
J Immunol Methods. 217 (1-2): 27-39. -
Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
Am J Pathol. 158 (1): 131-45. -
van Rijt, L. et al. (2002) Allergen-induced accumulation of airway dendritic cells is supported by an increase in CD31(hi)Ly-6C(neg) bone marrow precursors in a mouse model of asthma.
Blood. 100:3663-71. -
Tagoh, H. et al. (2002) Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells.
Genes Dev. 16:1721-37. -
Nikolic, T. et al. (2002) Developmental stages of myeloid dendritic cells in mouse bone marrow.
Int Immunol. 15:515-24.
View The Latest Product References
-
Baumeister, T. et al. (2003) Interleukin-3Ralpha+ myeloid dendritic cells and mast cells develop simultaneously from different bone marrow precursors in cultures with interleukin-3.
J Invest Dermatol. 121: 280-8. -
Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
J Leukoc Biol. 78: 845-52. -
Revermann, M. et al. (2010) Soluble epoxide hydrolase deficiency attenuates neointima formation in the femoral cuff model of hyperlipidemic mice.
Arterioscler Thromb Vasc Biol. 30: 909-14. -
Sumagin, R. and Sarelius, I.H. (2010) Intercellular adhesion molecule-1 enrichment near tricellular endothelial junctions is preferentially associated with leukocyte transmigration and signals for reorganization of these junctions to accommodate leukocyte passage.
J Immunol. 184: 5242-52. -
Ross, E.A. et al. (2011) CD31 Is Required on CD4+ T Cells To Promote T Cell Survival during Salmonella Infection.
J Immunol. 187: 1553-65. -
Thum, T. et al. (2011) Impairment of endothelial progenitor cell function and vascularization capacity by aldosterone in mice and humans.
Eur Heart J. 32: 1275-86. -
Thorp, E. et al. (2011) A reporter for tracking the UPR in vivo reveals patterns of temporal and cellular stress during atherosclerotic progression.
J Lipid Res. 52 (5): 1033-8. -
Schledzewski, K. et al. (2011) Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors.
J Clin Invest. 121: 703-14. -
Loureiro, J. et al. (2011) Blocking TGF-{beta}1 Protects the Peritoneal Membrane from Dialysate-Induced Damage.
J Am Soc Nephrol. 22: 1682-95. -
Matsakas, A. et al. (2012) Exercise training attenuates the hypermuscular phenotype and restores skeletal muscle function in the myostatin null mouse.
Exp Physiol. 97 (1): 125-40. -
Trottier MD et al. (2012) Enhancement of hematopoiesis and lymphopoiesis in diet-induced obese mice.
Proc Natl Acad Sci U S A. 109 (20): 7622-9. -
Moen, I. et al. (2012) Gene expression in tumor cells and stroma in dsRed 4T1 tumors in eGFP-expressing mice with and without enhanced oxygenation.
BMC Cancer. 12: 21. -
Trottier, M.D. et al. (2012) Enhanced production of early lineages of monocytic and granulocytic cells in mice with colitis
Proc Natl Acad Sci U S A.109: 16594-9. -
Kroon, P. et al. (2013) JAK-STAT blockade inhibits tumor initiation and clonogenic recovery of prostate cancer stem-like cells.
Cancer Res. 73 (16): 5288-98. -
Ono, N. et al. (2014) A subset of chondrogenic cells provides early mesenchymal progenitors in growing bones.
Nat Cell Biol. 16 (12): 1157-67. -
Eskilsson, A. et al. (2014) Distribution of microsomal prostaglandin E synthase-1 in the mouse brain.
J Comp Neurol. 522 (14): 3229-44. -
Nakamura, Y. et al. (2015) Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration.
FEBS Lett. 589 (11): 1257-65. -
Fraccarollo, D. et al. (2015) Efficacy of mineralocorticoid receptor antagonism in the acute myocardial infarction phase: eplerenone versus spironolactone.
ESC Heart Fail. 2 (3): 150-8. -
Stein-Merlob, A.F. et al. (2015) Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus Age-Dependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study.
Theranostics. 5 (12): 1317-27. -
Cao Y et al. (2016) IL-1β differently stimulates proliferation and multinucleation of distinct mouse bone marrow osteoclast precursor subsets.
J Leukoc Biol. 100 (3): 513-23. -
Ryan, T.E. et al. (2016) Mitochondrial therapy improves limb perfusion and myopathy following hindlimb ischemia.
J Mol Cell Cardiol. 97: 191-6. -
Shi, H. et al. (2016) Hiding inside? Intracellular expression of non-glycosylated c-kit protein in cardiac progenitor cells.
Stem Cell Res. 16 (3): 795-806. -
Chowdhury, B. et al. (2016) Hyaluronidase 2 (HYAL2) is expressed in endothelial cells, as well as some specialized epithelial cells, and is required for normal hyaluronan catabolism.
Histochem Cell Biol. 145 (1): 53-66. -
Yip, H.K. et al. (2016) Tissue plasminogen activator deficiency preserves neurological function and protects against murine acute ischemic stroke.
Int J Cardiol. 205: 133-41. -
Reigstad, I. et al. (2016) The Effect of Stromal Integrin β3-Deficiency on Two Different Tumors in Mice.
Cancers (Basel). 8 (1): pii: E14. -
Bongiorno, E.K. et al. (2017) Type 1 Immune Mechanisms Driven by the Response to Infection with Attenuated Rabies Virus Result in Changes in the Immune Bias of the Tumor Microenvironment and Necrosis of Mouse GL261 Brain Tumors.
J Immunol. 198 (11): 4513-4523. -
Cao, Y. et al. (2017) TNF-α has both stimulatory and inhibitory effects on mouse monocyte-derived osteoclastogenesis.
J Cell Physiol. 232 (12): 3273-85. -
Piro, J.R. et al. (2018) Inhibition of 2-AG hydrolysis differentially regulates blood brain barrier permeability after injury.
J Neuroinflammation. 15 (1): 142. -
Oikawa, S. et al. (2018) Role of endothelial microRNA-23 clusters in angiogenesis in vivo.
Am J Physiol Heart Circ Physiol. 315 (4): H838-H846. -
Tay, M.H.D. et al. (2019) Halted Lymphocyte Egress via Efferent Lymph Contributes to Lymph Node Hypertrophy During Hypercholesterolemia.
Front Immunol. 10: 575. -
Iring, A. et al. (2019) Shear stress-induced endothelial adrenomedullin signaling regulates vascular tone and blood pressure.
J Clin Invest. 129 (7): 2775-91. -
Ascone, G. et al. (2020) Increase in the Number of Bone Marrow Osteoclast Precursors at Different Skeletal Sites, Particularly in Long Bone and Jaw Marrow in Mice Lacking IL-1RA.
Int J Mol Sci. 21(11):3774. -
Patel, P. et al. (2023) Development of a Personalised Device for Systemic Magnetic Drug Targeting to Brain Tumours.
Nanotheranostics. 7 (1): 102-16. -
Eskilsson, A. et al. (2023) Prostaglandin production in brain endothelial cells during the initiation of fever.
Commun Integr Biol. 16 (1): 2166237. -
Yamashita, J. et al. (2021) Effect of bisphosphonates on healing of tooth extraction wounds in infectious osteomyelitis of the jaw.
Bone. 143: 115611. -
Molás, R.B. et al. (2020) The involvement of annexin A1 in human placental response to maternal Zika virus infection.
Antiviral Res. 179: 104809. -
Schuhmann, M.K. et al. (2020) CD84 Links T Cell and Platelet Activity in Cerebral Thrombo-Inflammation in Acute Stroke.
Circ Res. 127 (8): 1023-35. -
Wang, L. et al. (2024) Exposed Phosphatidylserine as a Biomarker for Clear Identification of Breast Cancer Brain Metastases in Mouse Models.
Cancers (Basel). 16 (17): 3088.
- Synonyms
- PECAM-1
- RRID
- AB_2161026
- UniProt
- Q08481
- Entrez Gene
- Pecam1
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005625 soluble fraction
- GO:0007266 Rho protein signal transduction
- GO:0030054 cell junction
- GO:0030334 regulation of cell migration
- GO:0030485 smooth muscle contractile fiber
- GO:0042060 wound healing
- GO:0045121 membrane raft
- View More GO Terms
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