CD31 antibody | ER-MP12

Rat anti Mouse CD31

Product Type
Monoclonal Antibody
Clone
ER-MP12
Isotype
IgG2a
Specificity
CD31

Product Code Applications Pack Size List Price Your Price Qty
MCA2388
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF IP 0.25 mg loader
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loader
MCA2388T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF IP 25 µg loader
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loader
MCA2388GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF IP 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD31 antibody, clone ER-MP12 recognizes mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.

CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
BALB/c macrophage precursor cell hybrids
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised rats were fused with the cells of the rat Y3-Ag1.2.3 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 Pack Size: 0.25 mg, 25 µg
1/10 Pack Size: 0.1 mg
1/200 Pack Size: 0.25 mg, 25 µg
1/100 Pack Size: 0.1 mg
Immunofluorescence
Immunohistology - Frozen 1 1/10 1/100
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Rat IgG2a Negative Control

References for CD31 antibody

  1. Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
    Eur J Immunol. 20 (1): 27-34.
  2. Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
    Am J Pathol. 158 (1): 131-45.
  3. de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.
    J Immunol Methods. 217 (1-2): 27-39.
  4. Revermann, M. et al. (2010) Soluble epoxide hydrolase deficiency attenuates neointima formation in the femoral cuff model of hyperlipidemic mice.
    Arterioscler Thromb Vasc Biol. 30: 909-14.
  5. Thorp, E. et al. (2011) A reporter for tracking the UPR in vivo reveals patterns of temporal and cellular stress during atherosclerotic progression.
    J Lipid Res. 52 (5): 1033-8.
  6. Thum, T. et al. (2011) Impairment of endothelial progenitor cell function and vascularization capacity by aldosterone in mice and humans.
    Eur Heart J. 32: 1275-86.
  7. Ross, E.A. et al. (2011) CD31 Is Required on CD4+ T Cells To Promote T Cell Survival during Salmonella Infection.
    J Immunol. 187: 1553-65.
  8. Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
    J Leukoc Biol. 78: 845-52.
  9. View The Latest Product References
  10. Schledzewski, K. et al. (2011) Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors.
    J Clin Invest. 121: 703-14.
  11. Sumagin, R. and Sarelius, I.H. (2010) Intercellular adhesion molecule-1 enrichment near tricellular endothelial junctions is preferentially associated with leukocyte transmigration and signals for reorganization of these junctions to accommodate leukocyte passage.
    J Immunol. 184: 5242-52.
  12. Loureiro, J. et al. (2011) Blocking TGF-{beta}1 Protects the Peritoneal Membrane from Dialysate-Induced Damage.
    J Am Soc Nephrol. 22: 1682-95.
  13. Matsakas, A. et al. (2012) Exercise training attenuates the hypermuscular phenotype and restores skeletal muscle function in the myostatin null mouse.
    Exp Physiol. 97 (1): 125-40.
  14. Baumeister, T. et al. (2003) Interleukin-3Ralpha+ myeloid dendritic cells and mast cells develop simultaneously from different bone marrow precursors in cultures with interleukin-3.
    J Invest Dermatol. 121: 280-8.
  15. Moen, I. et al. (2012) Gene expression in tumor cells and stroma in dsRed 4T1 tumors in eGFP-expressing mice with and without enhanced oxygenation.
    BMC Cancer. 12: 21.
  16. Trottier, M.D. et al. (2012) Enhanced production of early lineages of monocytic and granulocytic cells in mice with colitis
    Proc Natl Acad Sci U S A.109: 16594-9.
  17. Ling, V. et al. (1997) Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31).
    Eur J Immunol. 27:509-14.
  18. Nikolic, T. et al. (2002) Developmental stages of myeloid dendritic cells in mouse bone marrow.
    Int Immunol. 15:515-24.
  19. Tagoh, H. et al. (2002) Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells.
    Genes Dev. 16:1721-37.
  20. van Rijt, L. et al. (2002) Allergen-induced accumulation of airway dendritic cells is supported by an increase in CD31(hi)Ly-6C(neg) bone marrow precursors in a mouse model of asthma.
    Blood. 100:3663-71.
  21. van der Loo, J. et al. (1995) Identification of hematopoietic stem cell subsets on the basis of their primitiveness using antibody ER-MP12.
    Blood. 85:952-62.
  22. Fraccarollo, D. et al. (2015) Efficacy of mineralocorticoid receptor antagonism in the acute myocardial infarction phase: eplerenone versus spironolactone
    ESC Heart Failure. 2 (3): 150-8.
  23. Stein-Merlob, A.F. et al. (2015) Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus Age-Dependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study.
    Theranostics. 5 (12): 1317-27.
  24. Yip, H.K. et al. (2016) Tissue plasminogen activator deficiency preserves neurological function and protects against murine acute ischemic stroke.
    Int J Cardiol. 205: 133-41.
  25. Shi, H. et al. (2016) Hiding inside? Intracellular expression of non-glycosylated c-kit protein in cardiac progenitor cells.
    Stem Cell Res. 16 (3): 795-806.
  26. Ono, N. et al. (2014) A subset of chondrogenic cells provides early mesenchymal progenitors in growing bones.
    Nat Cell Biol. 16 (12): 1157-67.
  27. Kroon, P. et al. (2013) JAK-STAT blockade inhibits tumor initiation and clonogenic recovery of prostate cancer stem-like cells.
    Cancer Res. 73 (16): 5288-98.
  28. Trottier MD et al. (2012) Enhancement of hematopoiesis and lymphopoiesis in diet-induced obese mice.
    Proc Natl Acad Sci U S A. 109 (20): 7622-9.
  29. Ryan, T.E. et al. (2016) Mitochondrial therapy improves limb perfusion and myopathy following hindlimb ischemia.
    J Mol Cell Cardiol. 97: 191-6.
  30. Chowdhury, B. et al. (2016) Hyaluronidase 2 (HYAL2) is expressed in endothelial cells, as well as some specialized epithelial cells, and is required for normal hyaluronan catabolism.
    Histochem Cell Biol. 145 (1): 53-66.
  31. Nakamura, Y. et al. (2015) Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration.
    FEBS Lett. 589 (11): 1257-65.
  32. Reigstad, I. et al. (2016) The Effect of Stromal Integrin β3-Deficiency on Two Different Tumors in Mice.
    Cancers (Basel). 8 (1): pii: E14.
  33. Cao Y et al. (2016) IL-1β differently stimulates proliferation and multinucleation of distinct mouse bone marrow osteoclast precursor subsets.
    J Leukoc Biol. 100 (3): 513-23.
  34. Bongiorno, E.K. et al. (2017) Type 1 Immune Mechanisms Driven by the Response to Infection with Attenuated Rabies Virus Result in Changes in the Immune Bias of the Tumor Microenvironment and Necrosis of Mouse GL261 Brain Tumors.
    J Immunol. 198 (11): 4513-4523.
  35. Eskilsson, A. et al. (2014) Distribution of microsomal prostaglandin E synthase-1 in the mouse brain.
    J Comp Neurol. 522 (14): 3229-44.
  36. Cao, Y. et al. (2017) TNF-α has both stimulatory and inhibitory effects on mouse monocyte-derived osteoclastogenesis.
    J Cell Physiol. 232 (12): 3273-85.
  37. Piro, J.R. et al. (2018) Inhibition of 2-AG hydrolysis differentially regulates blood brain barrier permeability after injury.
    J Neuroinflammation. 15 (1): 142.
  38. Ascone, G. et al. (2020) Increase in the Number of Bone Marrow Osteoclast Precursors at Different Skeletal Sites, Particularly in Long Bone and Jaw Marrow in Mice Lacking IL-1RA.
    Int J Mol Sci. 21(11):3774.
  39. Tay, M.H.D. et al. (2019) Halted Lymphocyte Egress via Efferent Lymph Contributes to Lymph Node Hypertrophy During Hypercholesterolemia.
    Front Immunol. 10: 575.
  40. Iring, A. et al. (2019) Shear stress-induced endothelial adrenomedullin signaling regulates vascular tone and blood pressure.
    J Clin Invest. 129 (7): 2775-91.
  41. Oikawa, S. et al. (2018) Role of endothelial microRNA-23 clusters in angiogenesis in vivo.
    Am J Physiol Heart Circ Physiol. 315 (4): H838-H846.

Flow Cytometry

Immunofluorescence

Synonyms
PECAM-1
RRID
AB_2161026
UniProt
Q08481
Entrez Gene
Pecam1
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005625 soluble fraction
GO:0007266 Rho protein signal transduction
GO:0030054 cell junction
GO:0030334 regulation of cell migration
GO:0030485 smooth muscle contractile fiber
GO:0042060 wound healing
GO:0045121 membrane raft
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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