CD31 antibody | ER-MP12








Rat anti Mouse CD31:Alexa Fluor® 488
Rat anti Mouse CD31:Alexa Fluor® 647
Rat anti Mouse CD31:Biotin
Rat anti Mouse CD31:FITC
Rat anti Mouse CD31:Pacific Blue®
Rat anti Mouse CD31
Rat anti Mouse CD31:RPE
Rat anti Mouse CD31:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody
- Clone
- ER-MP12
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2388A488T | F | 25 Tests/0.25ml |
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MCA2388A488 | F | 100 Tests/1ml |
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MCA2388A647 | F | 100 Tests/1ml |
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MCA2388BT | F | 25 µg |
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MCA2388B | F | 0.1 mg |
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MCA2388F | F | 0.1 mg |
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MCA2388PB | F | 100 Tests/1ml |
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MCA2388T | C* F IF IP | 25 µg |
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MCA2388GA | C* F IF IP | 0.1 mg |
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MCA2388 | C* F IF IP | 0.25 mg |
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MCA2388PE | F | 100 Tests |
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MCA2388A700 | F | 100 Tests/1ml |
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CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Studies show that clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor®488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor®647- liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor®700 - liquid
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Carrier Free
- Yes
- Immunogen
- BALB/c macrophage precursor cell hybrids
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with the cells of the rat Y3-Ag1.2.3 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q08481
- Entrez Gene
- Pecam1
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005625 soluble fraction
- GO:0007266 Rho protein signal transduction
- GO:0030054 cell junction
- GO:0030334 regulation of cell migration
- GO:0030485 smooth muscle contractile fiber
- GO:0042060 wound healing
- GO:0045121 membrane raft
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD31 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 | |
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | |||
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
Flow Cytometry | 1/50 Pack Size: 25 µg, 0.25 mg 1/10 Pack Size: 0.1 mg |
1/200 Pack Size: 25 µg, 0.25 mg 1/100 Pack Size: 0.1 mg |
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Immunofluorescence | |||
Immunohistology - Frozen 1 | 1/10 | 1/100 | |
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use10ul of the suggested working dilution to label 106 cells in 100ul
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2a Negative Control:Alexa Fluor® 488 | MCA1212A488 | F | 100 Tests/1ml |
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Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml |
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Rat IgG2a Negative Control:FITC | MCA1212F | F | 100 Tests |
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Rat IgG2a Negative Control:Pacific Blue® | MCA1212PB | F | 100 Tests/1ml |
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Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
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Rat IgG2a Negative Control:Alexa Fluor® 700 | MCA1212A700 | F | 100 Tests/1ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Product Specific References
References for CD31 antibody
-
Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
Eur J Immunol. 20 (1): 27-34. -
Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
Am J Pathol. 158 (1): 131-45. -
de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.
J Immunol Methods. 217 (1-2): 27-39. -
Revermann, M. et al. (2010) Soluble epoxide hydrolase deficiency attenuates neointima formation in the femoral cuff model of hyperlipidemic mice.
Arterioscler Thromb Vasc Biol. 30: 909-14. -
Thorp, E. et al. (2011) A reporter for tracking the UPR in vivo reveals patterns of temporal and cellular stress during atherosclerotic progression.
J Lipid Res. 52 (5): 1033-8. -
Thum, T. et al. (2011) Impairment of endothelial progenitor cell function and vascularization capacity by aldosterone in mice and humans.
Eur Heart J. 32: 1275-86. -
Ross, E.A. et al. (2011) CD31 Is Required on CD4+ T Cells To Promote T Cell Survival during Salmonella Infection.
J Immunol. 187: 1553-65. -
Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
J Leukoc Biol. 78: 845-52. -
Schledzewski, K. et al. (2011) Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors.
J Clin Invest. 121: 703-14. -
Sumagin, R. and Sarelius, I.H. (2010) Intercellular adhesion molecule-1 enrichment near tricellular endothelial junctions is preferentially associated with leukocyte transmigration and signals for reorganization of these junctions to accommodate leukocyte passage.
J Immunol. 184: 5242-52. -
Loureiro, J. et al. (2011) Blocking TGF-{beta}1 Protects the Peritoneal Membrane from Dialysate-Induced Damage.
J Am Soc Nephrol. 22: 1682-95. -
Matsakas, A. et al. (2012) Exercise training attenuates the hypermuscular phenotype and restores skeletal muscle function in the myostatin null mouse.
Exp Physiol. 97 (1): 125-40. -
Baumeister, T. et al. (2003) Interleukin-3Ralpha+ myeloid dendritic cells and mast cells develop simultaneously from different bone marrow precursors in cultures with interleukin-3.
J Invest Dermatol. 121: 280-8. -
Moen, I. et al. (2012) Gene expression in tumor cells and stroma in dsRed 4T1 tumors in eGFP-expressing mice with and without enhanced oxygenation.
BMC Cancer. 12: 21. -
Trottier, M.D. et al. (2012) Enhanced production of early lineages of monocytic and granulocytic cells in mice with colitis
Proc Natl Acad Sci U S A.109: 16594-9. -
Ling, V. et al. (1997) Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31).
Eur J Immunol. 27:509-14. -
Nikolic, T. et al. (2002) Developmental stages of myeloid dendritic cells in mouse bone marrow.
Int Immunol. 15:515-24. -
Tagoh, H. et al. (2002) Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells.
Genes Dev. 16:1721-37. -
van Rijt, L. et al. (2002) Allergen-induced accumulation of airway dendritic cells is supported by an increase in CD31(hi)Ly-6C(neg) bone marrow precursors in a mouse model of asthma.
Blood. 100:3663-71. -
van der Loo, J. et al. (1995) Identification of hematopoietic stem cell subsets on the basis of their primitiveness using antibody ER-MP12.
Blood. 85:952-62. -
Fraccarollo, D. et al. (2015) Efficacy of mineralocorticoid receptor antagonism in the acute myocardial infarction phase: eplerenone versus spironolactone
ESC Heart Failure. 2 (3): 150-8. -
Stein-Merlob, A.F. et al. (2015) Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus Age-Dependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study.
Theranostics. 5 (12): 1317-27. -
Yip, H.K. et al. (2016) Tissue plasminogen activator deficiency preserves neurological function and protects against murine acute ischemic stroke.
Int J Cardiol. 205: 133-41. -
Shi, H. et al. (2016) Hiding inside? Intracellular expression of non-glycosylated c-kit protein in cardiac progenitor cells.
Stem Cell Res. 16 (3): 795-806. -
Ono, N. et al. (2014) A subset of chondrogenic cells provides early mesenchymal progenitors in growing bones.
Nat Cell Biol. 16 (12): 1157-67. -
Kroon, P. et al. (2013) JAK-STAT blockade inhibits tumor initiation and clonogenic recovery of prostate cancer stem-like cells.
Cancer Res. 73 (16): 5288-98. -
Trottier MD et al. (2012) Enhancement of hematopoiesis and lymphopoiesis in diet-induced obese mice.
Proc Natl Acad Sci U S A. 109 (20): 7622-9. -
Ryan, T.E. et al. (2016) Mitochondrial therapy improves limb perfusion and myopathy following hindlimb ischemia.
J Mol Cell Cardiol. 97: 191-6. -
Chowdhury, B. et al. (2016) Hyaluronidase 2 (HYAL2) is expressed in endothelial cells, as well as some specialized epithelial cells, and is required for normal hyaluronan catabolism.
Histochem Cell Biol. 145 (1): 53-66. -
Nakamura, Y. et al. (2015) Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration.
FEBS Lett. 589 (11): 1257-65. -
Reigstad, I. et al. (2016) The Effect of Stromal Integrin β3-Deficiency on Two Different Tumors in Mice.
Cancers (Basel). 8 (1): pii: E14. -
Cao Y et al. (2016) IL-1β differently stimulates proliferation and multinucleation of distinct mouse bone marrow osteoclast precursor subsets.
J Leukoc Biol. 100 (3): 513-23. -
Bongiorno, E.K. et al. (2017) Type 1 Immune Mechanisms Driven by the Response to Infection with Attenuated Rabies Virus Result in Changes in the Immune Bias of the Tumor Microenvironment and Necrosis of Mouse GL261 Brain Tumors.
J Immunol. 198 (11): 4513-4523. -
Eskilsson, A. et al. (2014) Distribution of microsomal prostaglandin E synthase-1 in the mouse brain.
J Comp Neurol. 522 (14): 3229-44. -
Cao, Y. et al. (2017) TNF-α has both stimulatory and inhibitory effects on mouse monocyte-derived osteoclastogenesis.
J Cell Physiol. 232 (12): 3273-85. -
Piro, J.R. et al. (2018) Inhibition of 2-AG hydrolysis differentially regulates blood brain barrier permeability after injury.
J Neuroinflammation. 15 (1): 142.
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