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CD3 antibody | 145-2C11

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Anti Mouse CD3 Antibody, clone 145-2C11 thumbnail image 1 Anti Mouse CD3 Antibody, clone 145-2C11 thumbnail image 2 Anti Mouse CD3 Antibody, clone 145-2C11 thumbnail image 3
Anti Mouse CD3 Antibody, clone 145-2C11 gallery image 1
Published customer image:
Hamster anti Mouse CD3 antibody, clone 145-2C11 (MCA2690) used for the identification of murine colonic lymphocytes by immunofluorescence.
Image caption:
Intestinal Myeloid Cells Respond Directly to Hh Signalling during Homeostasis and Inflammation (A–C) No DSS treatment. (D–F) 4 d of 3% DSS. (A, D) Few CD3+ T lymphocytes respond to Hh signalling during colonic homeostasis. Infiltrating T cells in DSS-induced ulcers do not express Gli1 or respond to Hh signalling. (B, E) Many but not all CD11b+ myeloid cells respond to Hh signalling and express LacZ. After 4 d of DSS, ulcers in Gli1+/lacZ animals are filled with myeloid cells, many of which respond to Hh signals. (C, F) CD11c+ dendritic cells respond directly to Hh signals during homeostasis and inflammation. Nuclear DAPI staining was performed but has been omitted to allow clear visualization of co-expression of nuclear LacZ and cell surface markers.

From: Lees CW, Zacharias WJ, Tremelling M, Noble CL, Nimmo ER, Tenesa A, et al. (2008)
Analysis of Germline GLI1 Variation Implicates Hedgehog Signalling in the Regulation of Intestinal Inflammatory Pathways.
PLoS Med 5(12): e239.
Anti Mouse CD3 Antibody, clone 145-2C11 gallery image 2
Figure A. RPE conjugated Rat anti Mouse CD22 (MCA2187PE) and A488 conjugated Hamster IgG isotype control (MCA2356A488). Figure B. RPE conjugated Rat anti Mouse CD22 (MCA2187PE) and A488 conjugated Hamster anti Mouse CD3 (MCA2690A488). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)
Anti Mouse CD3 Antibody, clone 145-2C11 gallery image 3
Published customer image:
Hamster anti Mouse CD3 antibody, clone 145-2C11 (MCA2690) used to identify T lymphocytes in mouse lamina propria by immunofluorescence.
Image caption:
Representative H&E staining of WT mice subjected to IRI or sham procedure with epithelial swelling and damage, cellular infiltrate, and signs of immune cell activation as well as apoptosis (left column). The immunohistological studies show constant numbers of CD3+ cells, a slightly decreased expression of F4/80 (tissue resident macrophages) and a strong increase of GR-1, which is expressed on neutrophils and activated macrophages. (GR-1, F4/80, or CD3 in red, DAPI positive nuclei in blue, autofluorescence of epithelial cells in green).

From: Yu Y, Feng X, Vieten G, Dippel S, Imvised T, Gueler F, et al. (2017)
Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.
PLoS ONE 12(7): e0181326.

Hamster anti Mouse CD3:Alexa Fluor®488

CD3 epsilon antibody, clone 145-2C11, is a 20kDa transmembrane protein on T cells, NKT cells and present at different levels of T cell differentiation. It's useful for in vitro blocking, activation assays, apoptosis induction and in vitro T cell depletions.

Hamster anti Mouse CD3

CD3 epsilon antibody, clone 145-2C11, is a 20kDa transmembrane protein on T cells, NKT cells and present at different levels of T cell differentiation. It's useful for in vitro blocking, activation assays, apoptosis induction and in vitro T cell depletions.

Product Type
Monoclonal Antibody
Clone
145-2C11
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA2690A488
Datasheet
F
SDS
1 ml loader

MCA2690T
Datasheet
C F IF IP WB
SDS
25 µg loader

MCA2690GA
Datasheet
C F IF IP WB
SDS
0.1 mg loader

MCA2690
Datasheet
C F IF IP WB
SDS
0.25 mg loader

Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.

CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.

Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 25 µg, 0.25 mg
0.09%Sodium Azide (NaN3)
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
Pack Size: 25 µg, 0.25 mg
IgG concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from hyperimmunized Armenian hamsters (Cricetulus migratorius) were fused with cells of the murine SP2/0 myeloma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P22646
Entrez Gene
Cd3e
GO Terms
GO:0001772 immunological synapse
GO:0002669 positive regulation of T cell anergy
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0032729 positive regulation of interferon-gamma production
GO:0032753 positive regulation of interleukin-4 production
GO:0042105 alpha-beta T cell receptor complex
GO:0045060 negative thymic T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045879 negative regulation of smoothened signaling pathway
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050852 T cell receptor signaling pathway
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 MCA2356A488 F 100 Tests/1ml loader
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Application Based External Images

Immunofluorescence

Product Specific References

References for CD3 antibody

  1. Leo, O. et al. (1987) Identification of a monoclonal antibody specific for a murine T3 polypeptide.
    Proc Natl Acad Sci U S A. 84 (5): 1374-8.
  2. Payer, E. et al. (1991) Circulating CD3+/T cell receptor V γ 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
    J Immunol. 146 (8): 2536-43.
  3. Salvadori, S. et al. (1994) Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.
    J Immunol. 153 (11): 5176-82.
  4. Podd BS et al. (2006) T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice.
    J Immunol. 176 (11): 6532-42.
  5. McDole JR et al. (2010) Rapid formation of extended processes and engagement of Theiler’s virus-infected neurons by CNS-infiltrating CD8 T cells.
    Am J Pathol. 177 (4): 1823-33.
  6. Lees, C.W. et al. (2008) Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways.
    PLoS Med. 5: e239.
  7. Klemann, C. et al. (2015) Interleukin-17, Produced by γδ-T Cells, Contributes to Hepatic Inflammation in a Mouse Model of Biliary Atresia and is Increased in Livers of Patients.
    Gastroenterology. pii: S0016-5085(15)01352-9.
  8. Parang, B. et al. (2016) Myeloid translocation genes differentially regulate colorectal cancer programs.
    Oncogene. 35 (49): 6341-9.
  9. Schuhmann, M.K. et al. (2017) Blocking of platelet glycoprotein receptor Ib reduces "thrombo-inflammation" in mice with acute ischemic stroke.
    J Neuroinflammation. 14 (1): 18.
  10. Yu, Y. et al. (2017) Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.
    PLoS One. 12 (7): e0181326.
  11. Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke.
    Pharmacol Res. 102: 298-307.
  12. Perrotta, M. et al. (2018) Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation.
    Cardiovasc Res. 114 (3): 456-67.

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