CD3 antibody | 145-2C11

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Hamster anti Mouse CD3:Alexa Fluor®488

CD3 epsilon antibody, clone 145-2C11, is a 20kDa transmembrane protein on T cells, NKT cells and present at different levels of T cell differentiation. It's useful for in vitro blocking, activation assays, apoptosis induction and in vitro T cell depletions.

Hamster anti Mouse CD3:Biotin

CD3 epsilon antibody, clone 145-2C11, is a 20kDa transmembrane protein on T cells, NKT cells and present at different levels of T cell differentiation. It's useful for in vitro blocking, activation assays, apoptosis induction and in vitro T cell depletions.

Hamster anti Mouse CD3

CD3 epsilon antibody, clone 145-2C11, is a 20kDa transmembrane protein on T cells, NKT cells and present at different levels of T cell differentiation. It's useful for in vitro blocking, activation assays, apoptosis induction and in vitro T cell depletions.

Product Type
Monoclonal Antibody
Clone
145-2C11
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2690A488 F 1 ml
MCA2690B F 0.1 mg
MCA2690T C F IF IP WB 25 µg
MCA2690GA C F IF IP WB 0.1 mg
MCA2690 C F IF IP WB 0.25 mg
Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.

CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.

Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
Pack Size: 25 µg, 0.25 mg
0.09%Sodium Azide (NaN3)
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
Pack Size: 25 µg, 0.25 mg
IgG concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from hyperimmunized Armenian hamsters (Cricetulus migratorius) were fused with cells of the murine SP2/0 myeloma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P22646 Related reagents
Entrez Gene
Cd3e Related reagents
GO Terms
GO:0001772 immunological synapse
GO:0002669 positive regulation of T cell anergy
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0032729 positive regulation of interferon-gamma production
GO:0032753 positive regulation of interleukin-4 production
GO:0042105 alpha-beta T cell receptor complex
GO:0045060 negative thymic T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045879 negative regulation of smoothened signaling pathway
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050852 T cell receptor signaling pathway
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 MCA2356A488 100 Tests/1ml F
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Immunofluorescence

Product Specific References

References for CD3 antibody

  1. Leo, O. et al. (1987) Identification of a monoclonal antibody specific for a murine T3 polypeptide.
    Proc Natl Acad Sci U S A. 84 (5): 1374-8.
  2. Payer, E. et al. (1991) Circulating CD3+/T cell receptor V γ 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
    J Immunol. 146 (8): 2536-43.
  3. Salvadori, S. et al. (1994) Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.
    J Immunol. 153 (11): 5176-82.
  4. Podd BS et al. (2006) T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice.
    J Immunol. 176 (11): 6532-42.
  5. McDole JR et al. (2010) Rapid formation of extended processes and engagement of Theiler’s virus-infected neurons by CNS-infiltrating CD8 T cells.
    Am J Pathol. 177 (4): 1823-33.
  6. Lees, C.W. et al. (2008) Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways.
    PLoS Med. 5: e239.
  7. Klemann, C. et al. (2015) Interleukin-17, Produced by γδ-T Cells, Contributes to Hepatic Inflammation in a Mouse Model of Biliary Atresia and is Increased in Livers of Patients.
    Gastroenterology. pii: S0016-5085(15)01352-9.
  8. Parang, B. et al. (2016) Myeloid translocation genes differentially regulate colorectal cancer programs.
    Oncogene. 35 (49): 6341-9.
  9. Schuhmann, M.K. et al. (2017) Blocking of platelet glycoprotein receptor Ib reduces "thrombo-inflammation" in mice with acute ischemic stroke.
    J Neuroinflammation. 14 (1): 18.
  10. Yu, Y. et al. (2017) Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.
    PLoS One. 12 (7): e0181326.
  11. Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke.
    Pharmacol Res. 102: 298-307.
  12. Perrotta, M. et al. (2018) Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation.
    Cardiovasc Res. 114 (3): 456-67.

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