CD3 antibody | 145-2C11
Hamster anti Mouse CD3 antibody, clone 145-2C11 (MCA2690) used for the identification of murine colonic lymphocytes by immunofluorescence.
Image caption:
Intestinal Myeloid Cells Respond Directly to Hh Signalling during Homeostasis and Inflammation (A–C) No DSS treatment. (D–F) 4 d of 3% DSS. (A, D) Few CD3+ T lymphocytes respond to Hh signalling during colonic homeostasis. Infiltrating T cells in DSS-induced ulcers do not express Gli1 or respond to Hh signalling. (B, E) Many but not all CD11b+ myeloid cells respond to Hh signalling and express LacZ. After 4 d of DSS, ulcers in Gli1+/lacZ animals are filled with myeloid cells, many of which respond to Hh signals. (C, F) CD11c+ dendritic cells respond directly to Hh signals during homeostasis and inflammation. Nuclear DAPI staining was performed but has been omitted to allow clear visualization of co-expression of nuclear LacZ and cell surface markers.
From: Lees CW, Zacharias WJ, Tremelling M, Noble CL, Nimmo ER, Tenesa A, et al. (2008)
Analysis of Germline GLI1 Variation Implicates Hedgehog Signalling in the Regulation of Intestinal Inflammatory Pathways.
PLoS Med 5(12): e239.
Hamster anti Mouse CD3 antibody, clone 145-2C11 (MCA2690) used to identify T lymphocytes in mouse lamina propria by immunofluorescence.
Image caption:
Representative H&E staining of WT mice subjected to IRI or sham procedure with epithelial swelling and damage, cellular infiltrate, and signs of immune cell activation as well as apoptosis (left column). The immunohistological studies show constant numbers of CD3+ cells, a slightly decreased expression of F4/80 (tissue resident macrophages) and a strong increase of GR-1, which is expressed on neutrophils and activated macrophages. (GR-1, F4/80, or CD3 in red, DAPI positive nuclei in blue, autofluorescence of epithelial cells in green).
From: Yu Y, Feng X, Vieten G, Dippel S, Imvised T, Gueler F, et al. (2017)
Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.
PLoS ONE 12(7): e0181326.
Hamster anti Mouse CD3
- Product Type
- Monoclonal Antibody
- Clone
- 145-2C11
- Isotype
- IgG
- Specificity
- CD3
Antibody Quality and Performance Guarantee
| Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell. CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- MCA2690T, MCA2690: 0.09% Sodium Azide (NaN3)
- MCA2690GA: 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
- Approx. Protein Concentrations
- MCA2690T, MCA2690: IgG concentration 1.0 mg/ml
- MCA2690GA: IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from hyperimmunized Armenian hamsters (Cricetulus migratorius) were fused with cells of the murine SP2/0 myeloma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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| List Price | Your Price | ||||||
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| Description | Hamster (Armenian) IgG Negative Control | ||||||
Source Reference
-
Leo, O. et al. (1987) Identification of a monoclonal antibody specific for a murine T3 polypeptide.
Proc Natl Acad Sci U S A. 84 (5): 1374-8.
References for CD3 antibody
-
Payer, E. et al. (1991) Circulating CD3+/T cell receptor V γ 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
J Immunol. 146 (8): 2536-43. -
Salvadori, S. et al. (1994) Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.
J Immunol. 153 (11): 5176-82. -
Lees, C.W. et al. (2008) Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways.
PLoS Med. 5: e239. -
McDole JR et al. (2010) Rapid formation of extended processes and engagement of Theiler’s virus-infected neurons by CNS-infiltrating CD8 T cells.
Am J Pathol. 177 (4): 1823-33. -
Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke.
Pharmacol Res. 102: 298-307. -
Klemann, C. et al. (2016) Interleukin 17, Produced by γδδ T Cells, Contributes to Hepatic Inflammation in a Mouse Model of Biliary Atresia and Is Increased in Livers of Patients.
Gastroenterology. 150 (1): 229-241.e5. -
Parang, B. et al. (2016) Myeloid translocation genes differentially regulate colorectal cancer programs.
Oncogene. 35 (49): 6341-9. -
Yu, Y. et al. (2017) Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.
PLoS One. 12 (7): e0181326.
View The Latest Product References
-
Schuhmann, M.K. et al. (2017) Blocking of platelet glycoprotein receptor Ib reduces "thrombo-inflammation" in mice with acute ischemic stroke.
J Neuroinflammation. 14 (1): 18. -
Perrotta, M. et al. (2018) Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation.
Cardiovasc Res. 114 (3): 456-67. -
Recasens, M. et al. (2019) Astrocyte-targeted IL-10 production decreases proliferation and induces a downregulation of activated microglia/macrophages after PPT.
Glia. 67 (4): 741-58. -
Ohmura, Y. et al. (2021) Natural Killer T Cells Are Involved in Atherosclerotic Plaque Instability in Apolipoprotein-E Knockout Mice.
Int J Mol Sci. 22(22):12451. -
Möhn, N. et al. (2020) Synthetic retinoid AM80 inhibits IL-17 production of gamma delta T cells and ameliorates biliary atresia in mice.
Liver Int. 40 (12): 3031-41. -
Shanaki-Bavarsad, M. et al. (2022) Astrocyte-targeted Overproduction of IL-10 Reduces Neurodegeneration after TBI.
Exp Neurobiol. 31 (3): 173-195. -
Manich, G. et al. (2024) Chronic IL-6 overproduction induces a tolerogenic response in aged mice after peripheral nerve injury
bioRχiv 13 Mar [Epub ahead of print].
- RRID
- AB_905951
- UniProt
- P22646
- Entrez Gene
- Cd3e
- GO Terms
- GO:0001772 immunological synapse
- GO:0002669 positive regulation of T cell anergy
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0009897 external side of plasma membrane
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032753 positive regulation of interleukin-4 production
- GO:0042105 alpha-beta T cell receptor complex
- View More GO Terms
- GO:0045060 negative thymic T cell selection
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045879 negative regulation of smoothened signaling pathway
- GO:0046641 positive regulation of alpha-beta T cell proliferation
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050850 positive regulation of calcium-mediated signaling
- GO:0050852 T cell receptor signaling pathway
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