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CD3 antibody | 145-2C11

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Anti Mouse CD3 Antibody, clone 145-2C11 thumbnail image 1 Anti Mouse CD3 Antibody, clone 145-2C11 thumbnail image 2 Anti Mouse CD3 Antibody, clone 145-2C11 thumbnail image 3
Anti Mouse CD3 Antibody, clone 145-2C11 gallery image 1
Published customer image:
Hamster anti Mouse CD3 antibody, clone 145-2C11 (MCA2690) used for the identification of murine colonic lymphocytes by immunofluorescence.
Image caption:
Intestinal Myeloid Cells Respond Directly to Hh Signalling during Homeostasis and Inflammation (A–C) No DSS treatment. (D–F) 4 d of 3% DSS. (A, D) Few CD3+ T lymphocytes respond to Hh signalling during colonic homeostasis. Infiltrating T cells in DSS-induced ulcers do not express Gli1 or respond to Hh signalling. (B, E) Many but not all CD11b+ myeloid cells respond to Hh signalling and express LacZ. After 4 d of DSS, ulcers in Gli1+/lacZ animals are filled with myeloid cells, many of which respond to Hh signals. (C, F) CD11c+ dendritic cells respond directly to Hh signals during homeostasis and inflammation. Nuclear DAPI staining was performed but has been omitted to allow clear visualization of co-expression of nuclear LacZ and cell surface markers.

From: Lees CW, Zacharias WJ, Tremelling M, Noble CL, Nimmo ER, Tenesa A, et al. (2008)
Analysis of Germline GLI1 Variation Implicates Hedgehog Signalling in the Regulation of Intestinal Inflammatory Pathways.
PLoS Med 5(12): e239.
Anti Mouse CD3 Antibody, clone 145-2C11 gallery image 2
Figure A. RPE conjugated Rat anti Mouse CD22 (MCA2187PE) and A488 conjugated Hamster IgG isotype control (MCA2356A488). Figure B. RPE conjugated Rat anti Mouse CD22 (MCA2187PE) and A488 conjugated Hamster anti Mouse CD3 (MCA2690A488). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)
Anti Mouse CD3 Antibody, clone 145-2C11 gallery image 3
Published customer image:
Hamster anti Mouse CD3 antibody, clone 145-2C11 (MCA2690) used to identify T lymphocytes in mouse lamina propria by immunofluorescence.
Image caption:
Representative H&E staining of WT mice subjected to IRI or sham procedure with epithelial swelling and damage, cellular infiltrate, and signs of immune cell activation as well as apoptosis (left column). The immunohistological studies show constant numbers of CD3+ cells, a slightly decreased expression of F4/80 (tissue resident macrophages) and a strong increase of GR-1, which is expressed on neutrophils and activated macrophages. (GR-1, F4/80, or CD3 in red, DAPI positive nuclei in blue, autofluorescence of epithelial cells in green).

From: Yu Y, Feng X, Vieten G, Dippel S, Imvised T, Gueler F, et al. (2017)
Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.
PLoS ONE 12(7): e0181326.

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Hamster anti Mouse CD3

Product Type
Monoclonal Antibody
Clone
145-2C11
Isotype
IgG
Specificity
CD3

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
MCA2690GA
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SDS Safety Datasheet SDS
C F IF IP WB 0.1 mg loader
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MCA2690T
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C F IF IP WB 25 µg loader
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MCA2690
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C F IF IP WB 0.25 mg loader
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Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.

CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.

Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Pack Size: 25 µg, 0.25 mg
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 1.0mg/ml
Pack Size: 25 µg, 0.25 mg
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from hyperimmunized Armenian hamsters (Cricetulus migratorius) were fused with cells of the murine SP2/0 myeloma.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

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References for CD3 antibody

  1. Tilley, S.L. et al. (2007) Retinoid-related orphan receptor γ controls immunoglobulin production and Th1/Th2 cytokine balance in the adaptive immune response to allergen.
    J Immunol. 178 (5): 3208-18.
  2. Leo, O. et al. (1987) Identification of a monoclonal antibody specific for a murine T3 polypeptide.
    Proc Natl Acad Sci U S A. 84 (5): 1374-8.
  3. Payer, E. et al. (1991) Circulating CD3+/T cell receptor V γ 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
    J Immunol. 146 (8): 2536-43.
  4. Salvadori, S. et al. (1994) Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.
    J Immunol. 153 (11): 5176-82.
  5. Podd BS et al. (2006) T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice.
    J Immunol. 176 (11): 6532-42.
  6. McDole JR et al. (2010) Rapid formation of extended processes and engagement of Theiler’s virus-infected neurons by CNS-infiltrating CD8 T cells.
    Am J Pathol. 177 (4): 1823-33.
  7. Lees, C.W. et al. (2008) Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways.
    PLoS Med. 5: e239.
  8. Klemann, C. et al. (2015) Interleukin-17, Produced by γδ-T Cells, Contributes to Hepatic Inflammation in a Mouse Model of Biliary Atresia and is Increased in Livers of Patients.
    Gastroenterology. pii: S0016-5085(15)01352-9.
    9. View The Latest Product References
  9. Parang, B. et al. (2016) Myeloid translocation genes differentially regulate colorectal cancer programs.
    Oncogene. 35 (49): 6341-9.
  10. Schuhmann, M.K. et al. (2017) Blocking of platelet glycoprotein receptor Ib reduces "thrombo-inflammation" in mice with acute ischemic stroke.
    J Neuroinflammation. 14 (1): 18.
  11. Yu, Y. et al. (2017) Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.
    PLoS One. 12 (7): e0181326.
  12. Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke.
    Pharmacol Res. 102: 298-307.
  13. Perrotta, M. et al. (2018) Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation.
    Cardiovasc Res. 114 (3): 456-67.
  14. Ohmura, Y. et al. (2021) Natural Killer T Cells Are Involved in Atherosclerotic Plaque Instability in Apolipoprotein-E Knockout Mice.
    Int J Mol Sci. 22(22):12451.

Immunofluorescence

RRID
AB_905951
UniProt
P22646
Entrez Gene
Cd3e
GO Terms
GO:0001772 immunological synapse
GO:0002669 positive regulation of T cell anergy
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0032729 positive regulation of interferon-gamma production
GO:0032753 positive regulation of interleukin-4 production
GO:0042105 alpha-beta T cell receptor complex
GO:0045060 negative thymic T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045879 negative regulation of smoothened signaling pathway
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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