CD279 antibody | RMP1-30

Rat anti Mouse CD279

Product Type
Monoclonal Antibody
Clone
RMP1-30
Isotype
IgG2b
Specificity
CD279

Product Code Applications Pack Size List Price Your Price Qty
MCA2464GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
MCA2464
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD279 antibody, clone RMP1-30 recognizes mouse CD279, a ~55 kDa cell surface protein, a member of the CD28/CTLA-4 family, otherwise known as Programmed Death-1 (PD-1). CD279 is expressed predominantly on activated T- and B- lymphocytes and on a subset of thymocytes.

CD279, an immunoinhibitory receptor, plays a critical role in peripheral tolerance induction and prevention of autoimmune disease (Ansari et al. 2003). Two members of the B7 family, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified as the ligands for CD279.

Rat anti Mouse CD279 antibody, clone RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig fusion proteins to PD-1 transfected BHK cells.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Pack Size: 0.25 mg
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
PD-1 transferred BHK cells.
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Pack Size: 0.25 mg
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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References for CD279 antibody

  1. Matsumoto, K. et al. (2004) B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.
    J Immunol. 172 (4): 2530-41.
  2. Bartkowiak, T. (2013) Novel Imaging-Based Techniques Reveal a Role for PD-1/PD-L1 in Tumor Immune Surveillance in the Lung.
    UT GSBS Dissertations and Theses (Open Access). Paper 354.
  3. Hu, Z. et al. (2013) Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect.
    J Immunol. 191 (1): 312-22.
  4. Groh, J. et al. (2018) Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.
    J Neuroinflammation. 15 (1): 194.

Flow Cytometry

Synonyms
PD-1
RRID
AB_961509
UniProt
Q02242
Entrez Gene
Pdcd1
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0009897 external side of plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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