CD254 antibody | IK22/5
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Rat anti Mouse CD254
- Product Type
- Monoclonal Antibody
- Clone
- IK22/5
- Isotype
- IgG2a
- Specificity
- CD254
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD254 antibody, clone IK22/5 recognises CD254, otherwise known as Receptor activator of nuclear factor kappa B ligand (RANKL) or TNF-related activation-induced cytokine (TRANCE), a member of the tumour necrosis factor ligand superfamily. CD254 is a cytokine that binds osteoprotegerin or RANK and plays an important role in bone metabolism, where it acts as an osteoclast differentiation and activation factor. Overproduction of CD254 is implicated in a range of degenerative bone diseases. CD254 is also expressed by T helper cells and augments the ability of dendritic cells to stimulate naïve T cell proliferation. It may play a role in regulating interactions between T cells and dendritic cells and in the regulation of T cell-dependent immune responses. Rat anti Mouse CD254 antibody, clone IK22/5 blocks ligand binding. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/20 |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for CD254 antibody
-
Miyahira, Y. et al. (2003) Cutting edge: a potent adjuvant effect of ligand to receptor activator of NF-kappa B gene for inducing antigen-specific CD8+ T cell response by DNA and viral vector vaccination.
J Immunol. 171: 6344-8. -
Miyahira, Y. et al. (2005) Immune responses against a single CD8+-T-cell epitope induced by virus vector vaccination can successfully control Trypanosoma cruzi infection.
Infect Immun. 73: 7356-65. -
Kamijo, S. et al. (2006) Amelioration of bone loss in collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody.
Biochem Biophys Res Commun. 347: 124-32. -
Nair, V.R. et al. (2016) Microfold Cells Actively Translocate
Mycobacterium tuberculosis to Initiate Infection.
Cell Rep. 16 (5): 1253-8.
- Synonyms
- sRANKL
- RRID
- AB_2206081
- UniProt
- O35235
- Entrez Gene
- Tnfsf11
- GO Terms
- GO:0005886 plasma membrane
- GO:0001503 ossification
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0005737 cytoplasm
- GO:0006955 immune response
- View More GO Terms
- GO:0009887 organ morphogenesis
- GO:0048535 lymph node development
- GO:0030316 osteoclast differentiation
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0033598 mammary gland epithelial cell proliferation
- GO:0051260 protein homooligomerization
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045453 bone resorption
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0051466 positive regulation of corticotropin-releasing hormone secretion
- GO:0051897 positive regulation of protein kinase B signaling cascade
- GO:0060749 mammary gland alveolus development
- GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
MCA4711
151937
166800
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