CD254 antibody | IK22/5

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Rat anti Mouse CD254

Product Type
Monoclonal Antibody
Clone
IK22/5
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4711 C F IF IP WB 0.25 mg
Rat anti Mouse CD254 antibody, clone IK22/5 recognises CD254, otherwise known as Receptor activator of nuclear factor kappa B ligand (RANKL) or TNF-related activation-induced cytokine (TRANCE), a member of the tumour necrosis factor ligand superfamily. CD254 is a cytokine that binds osteoprotegerin or RANK and plays an important role in bone metabolism, where it acts as an osteoclast differentiation and activation factor. Overproduction of CD254 is implicated in a range of degenerative bone diseases.

CD254 is also expressed by T helper cells and augments the ability of dendritic cells to stimulate naïve T cell proliferation. It may play a role in regulating interactions between T cells and dendritic cells and in the regulation of T cell-dependent immune responses.

Rat anti Mouse CD254 antibody, clone IK22/5 blocks ligand binding.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O35235 Related reagents
Entrez Gene
Tnfsf11 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001503 ossification
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0006955 immune response
GO:0009887 organ morphogenesis
GO:0048535 lymph node development
GO:0030316 osteoclast differentiation
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0033598 mammary gland epithelial cell proliferation
GO:0051260 protein homooligomerization
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045453 bone resorption
GO:0045672 positive regulation of osteoclast differentiation
GO:0051466 positive regulation of corticotropin-releasing hormone secretion
GO:0051897 positive regulation of protein kinase B signaling cascade
GO:0060749 mammary gland alveolus development
GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
Regulatory
For research purposes only

Applications of CD254 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control MCA1212 1 ml E F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD254 antibody

  1. Kamijo, S. et al. (2006) Amelioration of bone loss in collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody.
    Biochem Biophys Res Commun. 347: 124-32.
  2. Miyahira, Y. et al. (2003) Cutting edge: a potent adjuvant effect of ligand to receptor activator of NF-kappa B gene for inducing antigen-specific CD8+ T cell response by DNA and viral vector vaccination.
    J Immunol. 171: 6344-8.
  3. Miyahira, Y. et al. (2005) Immune responses against a single CD8+-T-cell epitope induced by virus vector vaccination can successfully control Trypanosoma cruzi infection.
    Infect Immun. 73: 7356-65.
  4. Knoop, K. et al. (2009) RANKL is necessary and sufficient to initiate development of antigen-sampling M cells in the intestinal epithelium.
    J Immunol. 183:5738-47.
  5. Nair, V.R. et al. (2016) Microfold Cells Actively Translocate
    Mycobacterium tuberculosis
    to Initiate Infection.
    Cell Rep. 16 (5): 1253-1258.