CD254 antibody | IK22/5
|Rat anti Mouse CD254 antibody, clone IK22/5 recognises CD254, otherwise known as Receptor activator of nuclear factor kappa B ligand (RANKL) or TNF-related activation-induced cytokine (TRANCE), a member of the tumour necrosis factor ligand superfamily. CD254 is a cytokine that binds osteoprotegerin or RANK and plays an important role in bone metabolism, where it acts as an osteoclast differentiation and activation factor. Overproduction of CD254 is implicated in a range of degenerative bone diseases.
CD254 is also expressed by T helper cells and augments the ability of dendritic cells to stimulate naïve T cell proliferation. It may play a role in regulating interactions between T cells and dendritic cells and in the regulation of T cell-dependent immune responses.
Rat anti Mouse CD254 antibody, clone IK22/5 blocks ligand binding.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD254 antibody
Kamijo, S. et al. (2006) Amelioration of bone loss in collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody.
Biochem Biophys Res Commun. 347: 124-32.
Miyahira, Y. et al. (2003) Cutting edge: a potent adjuvant effect of ligand to receptor activator of NF-kappa B gene for inducing antigen-specific CD8+ T cell response by DNA and viral vector vaccination.
J Immunol. 171: 6344-8.
Miyahira, Y. et al. (2005) Immune responses against a single CD8+-T-cell epitope induced by virus vector vaccination can successfully control Trypanosoma cruzi infection.
Infect Immun. 73: 7356-65.
Knoop, K. et al. (2009) RANKL is necessary and sufficient to initiate development of antigen-sampling M cells in the intestinal epithelium.
J Immunol. 183:5738-47.
Nair, V.R. et al. (2016) Microfold Cells Actively Translocate
Mycobacterium tuberculosis to Initiate Infection.
Cell Rep. 16 (5): 1253-1258.
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0001503 ossification
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0005737 cytoplasm
- GO:0006955 immune response
- View More GO Terms
- GO:0009887 organ morphogenesis
- GO:0048535 lymph node development
- GO:0030316 osteoclast differentiation
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0033598 mammary gland epithelial cell proliferation
- GO:0051260 protein homooligomerization
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045453 bone resorption
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0051466 positive regulation of corticotropin-releasing hormone secretion
- GO:0051897 positive regulation of protein kinase B signaling cascade
- GO:0060749 mammary gland alveolus development
- GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
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