CD253 antibody | N2B2

Rat anti Mouse CD253
- Product Type
- Monoclonal Antibody
- Clone
- N2B2
- Isotype
- IgG2a
- Specificity
- CD253
Rat anti Mouse CD253 antibody, clone N2B2 recognizes mouse CD253, also known as TNF-related apoptosis-inducing ligand or TRAIL, a member of the tumour necrosis factor ligand superfamily. When bound to the death receptors DR4 and DR5 caspase-8-dependent apoptosis is induced. However, CD253 might also be bound by the decoy receptor DcR1 which cannot induce apoptosis, and DcR2 which activates NF-kappa-B. Rat anti Mouse CD253 antibody, clone N2B2 has been reported to block NK cell cytotoxicity in vitro (Kayagaki et al. 1999). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse CD253-transfected 2PK-3 cells.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Splenocytes from an immunized F344/DuCrj rat were fused with cells of the mouse P3U1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/5 | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Description | Rat IgG2a Negative Control |
Source Reference
-
Kayagaki, N. et al. (1999) Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells.
J Immunol. 163 (4): 1906-13.
References for CD253 antibody
-
Smyth, M.J. et al. (2003) Nature's TRAIL--on a path to cancer immunotherapy.
Immunity. 18 (1): 1-6.
- Synonyms
- TRAIL
- RRID
- AB_2256260
- UniProt
- P50592
- Entrez Gene
- Tnfsf10
- GO Terms
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
MCA4710GA

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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