CD253 antibody | N2B2

Rat anti Mouse CD253

Product Type
Monoclonal Antibody
Clone
N2B2
Isotype
IgG2a
Specificity
CD253

Product Code Applications Pack Size List Price Your Price Qty
MCA4710GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD253 antibody, clone N2B2 recognizes mouse CD253, also known as TNF-related apoptosis-inducing ligand or TRAIL, a member of the tumour necrosis factor ligand superfamily. When bound to the death receptors DR4 and DR5 caspase-8-dependent apoptosis is induced. However, CD253 might also be bound by the decoy receptor DcR1 which cannot induce apoptosis, and DcR2 which activates NF-kappa-B.

Rat anti Mouse CD253 antibody, clone N2B2 has been reported to block NK cell cytotoxicity in vitro (Kayagaki et al. 1999).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Mouse CD253-transfected 2PK-3 cells.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Fusion Partners
Splenocytes from an immunized F344/DuCrj rat were fused with cells of the mouse P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Rat IgG2a Negative Control

Source Reference

  1. Kayagaki, N. et al. (1999) Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells.
    J Immunol. 163 (4): 1906-13.

References for CD253 antibody

  1. Smyth, M.J. et al. (2003) Nature's TRAIL--on a path to cancer immunotherapy.
    Immunity. 18 (1): 1-6.

Synonyms
TRAIL
RRID
AB_2256260
UniProt
P50592
Entrez Gene
Tnfsf10
GO Terms
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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