CD253 antibody | N2B2
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Rat anti Mouse CD253
- Product Type
- Monoclonal Antibody
- Clone
- N2B2
- Isotype
- IgG2a
- Specificity
- CD253
Antibody Quality and 
Unrivaled 
| Rat anti Mouse CD253 antibody, clone N2B2 recognizes mouse CD253, also known as TNF-related apoptosis-inducing ligand or TRAIL, a member of the tumour necrosis factor ligand superfamily. When bound to the death receptors DR4 and DR5 caspase-8-dependent apoptosis is induced. However, CD253 might also be bound by the decoy receptor DcR1 which cannot induce apoptosis, and DcR2 which activates NF-kappa-B. Rat anti Mouse CD253 antibody, clone N2B2 has been reported to block NK cell cytotoxicity in vitro (Kayagaki et al. 1999). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse CD253-transfected 2PK-3 cells.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Splenocytes from an immunized F344/DuCrj rat were fused with cells of the mouse P3U1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/5 | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
Source Reference
-
Kayagaki, N. et al. (1999) Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells.
J Immunol. 163 (4): 1906-13.
References for CD253 antibody
-
Smyth, M.J. et al. (2003) Nature's TRAIL--on a path to cancer immunotherapy.
Immunity. 18 (1): 1-6.
- Synonyms
- TRAIL
- RRID
- AB_2256260
- UniProt
- P50592
- Entrez Gene
- Tnfsf10
- GO Terms
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
View more products with CD253 specificity
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