CD25 antibody | PC61.5.3
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F ResetRat anti Mouse CD25
- Product Type
- Monoclonal Antibody
- Clone
- PC61.5.3
- Isotype
- IgG1
- Specificity
- CD25
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD25 antibody, clone PC61.5.3 reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- B6.1 CTL cell line.
- Approx. Protein Concentrations
- Pack Size: 0.25 mgIgG concentration 1.0 mg/mlPack Size: 0.1 mgIgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized OFA rats were fused with cells of the P3X63Ag8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl.
References for CD25 antibody
-
Moreau, J.L. et al. (1987) Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site.
Eur J Immunol. 17 (7): 929-35. -
Hashimoto, N. et al. (1986) Dissociation of interleukin 2-dependent and -independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody.
Eur J Immunol. 16 (3): 317-20. -
Lowenthal, J.W. et al. (1985) High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61.
J Immunol. 135 (6): 3988-94. -
Ceredig, R. et al. (1985) Expression of interleukin-2 receptor as a differentiation marker on intrathymic stem cells.
Nature 314: 98-100. -
Yaqoob, P. & Calder, P.C. (1997) Glutamine requirement of proliferating T lymphocytes.
Nutrition. 13 (7-8): 646-51. -
Scotland, R.S. et al. (2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.
Blood. 118 (22): 5918-27. -
Karali, D. et al. (2016) T cell regulation by Phlomis lanata protein extracts in mice.
Pharm Biol. 54 (2): 207-14. -
Szuster-Ciesielska, A. et al. (2019) Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2 of Plasmodium falciparum in a Mouse Model.
J Immunol Res. 2019: 9264217.
View The Latest Product References
-
Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
Vet Immunol Immunopathol. 198: 44-53. -
Arad, T. et al. (2021) CD200 -dependent and -independent immune-modulatory functions of neural stem cells.
Stem Cell Res. 56: 102559. -
Roca, C.P. et al. (2023) A cross entropy test allows quantitative statistical comparison of t-SNE and UMAP representations
Cell Reports Methods. 3 (1): 100390.
- Synonyms
- IL-2R Alpha Chain
- RRID
- AB_2125347
- UniProt
- P01590
- Entrez Gene
- Il2ra
- GO Terms
- GO:0016021 integral to membrane
- GO:0004911 interleukin-2 receptor activity
- GO:0006924 activation-induced cell death of T cells
- GO:0009897 external side of plasma membrane
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042130 negative regulation of T cell proliferation
- GO:0043065 positive regulation of apoptosis
- GO:0046013 regulation of T cell homeostatic proliferation
- GO:0050728 negative regulation of inflammatory response
- View More GO Terms
MCA1260G
150150
MCA1260GA
150151
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